Elimination of carbon catabolite repression in Clostridium acetobutylicum—a journey toward simultaneous use of xylose and glucose

2015 ◽  
Vol 99 (18) ◽  
pp. 7579-7588 ◽  
Author(s):  
Mark Bruder ◽  
Murray Moo-Young ◽  
Duane A. Chung ◽  
C. Perry Chou
Keyword(s):  
Catabolite Repression ◽  
Clostridium Acetobutylicum ◽  
Carbon Catabolite Repression ◽  
Simultaneous Use

Carbon catabolite repression gene creA regulates morphology, aflatoxin biosynthesis and virulence in Aspergillus flavus

2018 ◽  
Vol 115 ◽  
pp. 41-51 ◽  
Author(s):  
Opemipo Esther Fasoyin ◽  
Bin Wang ◽  
Mengguang Qiu ◽  
Xiaoyun Han ◽  
Kuang-Ren Chung ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Aflatoxin Biosynthesis

Carbon catabolite repression occurrence in photo fermentation of ethanol-rich substrates

2021 ◽  
Vol 297 ◽  
pp. 113371
Author(s):  
Grazia Policastro ◽  
Marco Giugliano ◽  
Vincenzo Luongo ◽  
Raffaele Napolitano ◽  
Massimiliano Fabbricino
Keyword(s):  
Catabolite Repression ◽  
Carbon Catabolite Repression

scholarly journals Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440

2017 ◽  
Vol 5 ◽  
pp. 19-25 ◽  
Author(s):  
Christopher W. Johnson ◽  
Paul E. Abraham ◽  
Jeffrey G. Linger ◽  
Payal Khanna ◽  
Robert L. Hettich ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Global Regulator ◽  
Pseudomonas Putida Kt2440

scholarly journals Substrate-Induced Production and Secretion of Cellulases by Clostridium acetobutylicum

2004 ◽  
Vol 70 (9) ◽  
pp. 5238-5243 ◽  
Author(s):  
Ana M. López-Contreras ◽  
Krisztina Gabor ◽  
Aernout A. Martens ◽  
Bernadet A. M. Renckens ◽  
Pieternel A. M. Claassen ◽  
...  
Keyword(s):  
Catabolite Repression ◽  
Clostridium Acetobutylicum ◽  
Glycoside Hydrolase ◽  
Protein A ◽  
Cellulase Activity ◽  
Glycoside Hydrolase Family ◽  
Extracellular Production ◽  
Encoding Genes ◽  
Glycoside Hydrolase Family 48 ◽  
Hydrolase Family

ABSTRACT Clostridium acetobutylicum ATCC 824 is a solventogenic bacterium that grows heterotrophically on a variety of carbohydrates, including glucose, cellobiose, xylose, and lichenan, a linear polymer of β-1,3- and β-1,4-linked β-d-glucose units. C. acetobutylicum does not degrade cellulose, although its genome sequence contains several cellulase-encoding genes and a complete cellulosome cluster of cellulosome genes. In the present study, we demonstrate that a low but significant level of induction of cellulase activity occurs during growth on xylose or lichenan. The celF gene, located in the cellulosome-like gene cluster and coding for a unique cellulase that belongs to glycoside hydrolase family 48, was cloned in Escherichia coli, and antibodies were raised against the overproduced CelF protein. A Western blot analysis suggested a possible catabolite repression by glucose or cellobiose and an up-regulation by lichenan or xylose of the extracellular production of CelF by C. acetobutylicum. Possible reasons for the apparent inability of C. acetobutylicum to degrade cellulose are discussed.

scholarly journals Arabinose-Induced Catabolite Repression as a Mechanism for Pentose Hierarchy Control inClostridium acetobutylicumATCC 824

mSystems ◽  
2018 ◽  
Vol 3 (5) ◽  
Author(s):  
Matthew D. Servinsky ◽  
Rebecca L. Renberg ◽  
Matthew A. Perisin ◽  
Elliot S. Gerlach ◽  
Sanchao Liu ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Genetic Engineering ◽  
Catabolite Repression ◽  
Clostridium Acetobutylicum ◽  
Regulation Mechanism ◽  
Mrna Levels ◽  
Chemical Production ◽  
Content Type ◽  
Commodity Chemicals ◽  
Pentose Utilization

ABSTRACTBacterial fermentation of carbohydrates from sustainable lignocellulosic biomass into commodity chemicals by the anaerobic bacteriumClostridium acetobutylicumis a promising alternative source to fossil fuel-derived chemicals. Recently, it was demonstrated that xylose is not appreciably fermented in the presence of arabinose, revealing a hierarchy of pentose utilization in this organism (L. Aristilde, I. A. Lewis, J. O. Park, and J. D. Rabinowitz, Appl Environ Microbiol 81:1452–1462, 2015,https://doi.org/10.1128/AEM.03199-14). The goal of the current study is to characterize the transcriptional regulation that occurs and perhaps drives this pentose hierarchy. Carbohydrate consumption rates showed that arabinose, like glucose, actively represses xylose utilization in cultures fermenting xylose. Further, arabinose addition to xylose cultures led to increased acetate-to-butyrate ratios, which indicated a transition of pentose catabolism from the pentose phosphate pathway to the phosphoketolase pathway. Transcriptome sequencing (RNA-Seq) confirmed that arabinose addition to cells actively growing on xylose resulted in increased phosphoketolase (CA_C1343) mRNA levels, providing additional evidence that arabinose induces this metabolic switch. A significant overlap in differentially regulated genes after addition of arabinose or glucose suggested a common regulation mechanism. A putative open reading frame (ORF) encoding a potential catabolite repression phosphocarrier histidine protein (Crh) was identified that likely participates in the observed transcriptional regulation. These results substantiate the claim that arabinose is utilized preferentially over xylose inC. acetobutylicumand suggest that arabinose can activate carbon catabolite repression via Crh. Furthermore, they provide valuable insights into potential mechanisms for altering pentose utilization to modulate fermentation products for chemical production.IMPORTANCEClostridium acetobutylicumcan ferment a wide variety of carbohydrates to the commodity chemicals acetone, butanol, and ethanol. Recent advances in genetic engineering have expanded the chemical production repertoire ofC. acetobutylicumusing synthetic biology. Due to its natural properties and genetic engineering potential, this organism is a promising candidate for converting biomass-derived feedstocks containing carbohydrate mixtures to commodity chemicals via natural or engineered pathways. Understanding how this organism regulates its metabolism during growth on carbohydrate mixtures is imperative to enable control of synthetic gene circuits in order to optimize chemical production. The work presented here unveils a novel mechanism via transcriptional regulation by a predicted Crh that controls the hierarchy of carbohydrate utilization and is essential for guiding robust genetic engineering strategies for chemical production.

scholarly journals Onset of Carbon Catabolite Repression inAspergillus nidulans

2003 ◽  
Vol 278 (14) ◽  
pp. 11849-11857 ◽  
Author(s):  
Michel Flipphi ◽  
Peter J. I. van de Vondervoort ◽  
George J. G. Ruijter ◽  
Jaap Visser ◽  
Herbert N. Arst ◽  
...  
Keyword(s):  
Catabolite Repression ◽  
Carbon Catabolite Repression
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