scholarly journals Erratum to: Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens

2015 ◽  
Vol 99 (19) ◽  
pp. 8321-8321
Author(s):  
Yang Wang ◽  
Li Yi ◽  
Shaohui Wang ◽  
Chengping Lu ◽  
Chan Ding
Keyword(s):  
Functional Gene ◽  
Gene Analysis ◽  
Microbial Pathogens ◽  
Functional Gene Analysis ◽  
Transcribed Sequences

Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens

2014 ◽  
Vol 98 (24) ◽  
pp. 9983-9992 ◽  
Author(s):  
Yang Wang ◽  
Li Yi ◽  
Shaohui Wang ◽  
Chengping Lu ◽  
Chan Ding
Keyword(s):  
Functional Gene ◽  
Gene Analysis ◽  
Microbial Pathogens ◽  
Functional Gene Analysis ◽  
Transcribed Sequences

scholarly journals Clonal sector analysis and cell ablation confirm a function for DORNROESCHEN-LIKE in founder cells and the vasculature in Arabidopsis

Planta ◽  
2021 ◽  
Vol 253 (2) ◽  
Author(s):  
Dorothea Glowa ◽  
Petra Comelli ◽  
John W. Chandler ◽  
Wolfgang Werr
Keyword(s):  
Transcription Factor ◽  
Functional Gene ◽  
Gene Analysis ◽  
Cell Ablation ◽  
Sector Analysis ◽  
Founder Cells ◽  
Conventional Methods ◽  
Functional Gene Analysis ◽  
Lineage Analysis ◽  
In Cells

Abstract Main conclusion Inducible lineage analysis and cell ablation via conditional toxin expression in cells expressing the DORNRÖSCHEN-LIKE transcription factor represent an effective and complementary adjunct to conventional methods of functional gene analysis. Abstract Classical methods of functional gene analysis via mutational and expression studies possess inherent limitations, and therefore, the function of a large proportion of transcription factors remains unknown. We have employed two complementary, indirect methods to obtain functional information for the AP2/ERF transcription factor DORNRÖSCHEN-LIKE (DRNL), which is dynamically expressed in flowers and marks lateral organ founder cells. An inducible, two-component Cre–Lox system was used to express beta-glucuronidase GUS in cells expressing DRNL, to perform a sector analysis that reveals lineages of cells that transiently expressed DRNL throughout plant development. In a complementary approach, an inducible system was used to ablate cells expressing DRNL using diphtheria toxin A chain, to visualise the phenotypic consequences. These complementary analyses demonstrate that DRNL functionally marks founder cells of leaves and floral organs. Clonal sectors also included the vasculature of the leaves and petals, implicating a previously unidentified role for DRNL in provasculature development, which was confirmed in cotyledons by closer analysis of drnl mutants. Our findings demonstrate that inducible gene-specific lineage analysis and cell ablation via conditional toxin expression represent an effective and informative adjunct to conventional methods of functional gene analysis.

scholarly journals Functional gene analysis of individual response to challenge of SIVmac239 in M. mulatta PBMC culture

Virology ◽  
2006 ◽  
Vol 348 (1) ◽  
pp. 242-252 ◽  
Author(s):  
Matthew J. Thomas ◽  
Michael B. Agy ◽  
Sean C. Proll ◽  
Bryan W. Paeper ◽  
Yu Li ◽  
...  
Keyword(s):  
Functional Gene ◽  
Gene Analysis ◽  
Individual Response ◽  
Pbmc Culture ◽  
Functional Gene Analysis

scholarly journals A Multicolor Panel of Novel Lentiviral “Gene Ontology” (LeGO) Vectors for Functional Gene Analysis

2008 ◽  
Vol 16 (4) ◽  
pp. 698-706 ◽  
Author(s):  
Kristoffer Weber ◽  
Udo Bartsch ◽  
Carol Stocking ◽  
Boris Fehse
Keyword(s):  
Gene Ontology ◽  
Functional Gene ◽  
Gene Analysis ◽  
Functional Gene Analysis

Bacterial Community Profiling and Predictive Functional Gene Analysis during Petroleum Biodegradation

2020 ◽  
Author(s):  
Chioma Blaise Chikere ◽  
Charles Chibueze Ezekoye ◽  
Gideon Chijioke Okpokwasili
Keyword(s):  
Bacterial Community ◽  
Functional Gene ◽  
Gene Analysis ◽  
Community Profiling ◽  
Functional Gene Analysis

Characterization and functional gene analysis of a newly isolated indole-degrading bacterium Burkholderia sp. IDO3

2019 ◽  
Vol 367 ◽  
pp. 144-151 ◽  
Author(s):  
Qiao Ma ◽  
Ziyan Liu ◽  
Bingyu Yang ◽  
Chunxiao Dai ◽  
Yuanyuan Qu
Keyword(s):  
Functional Gene ◽  
Gene Analysis ◽  
Degrading Bacterium ◽  
Functional Gene Analysis
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