Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells

2018 ◽  
Vol 102 (11) ◽  
pp. 4729-4739 ◽  
Author(s):  
Joo-Hyoung Lee ◽  
Jong-Ho Park ◽  
Sun-Hye Park ◽  
Sun-Hong Kim ◽  
Jee Yon Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Amplification ◽  
Cho Cells ◽  
Protein Production ◽  
Transient Gene Expression ◽  
Foreign Protein

Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells

2015 ◽  
Vol 37 (12) ◽  
pp. 2379-2386 ◽  
Author(s):  
Yashas Rajendra ◽  
Maria D. Hougland ◽  
Matthew G. Schmitt ◽  
Gavin C. Barnard
Keyword(s):  
Gene Expression ◽  
Cho Cells ◽  
Transient Gene Expression ◽  
Transcriptional Targeting

Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells

2001 ◽  
Vol 75 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Petra Meissner ◽  
Horst Pick ◽  
Alexandra Kulangara ◽  
Philippe Chatellard ◽  
Kirstin Friedrich ◽  
...  
Keyword(s):  
Gene Expression ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Transient Gene Expression

NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells

2015 ◽  
Vol 10 (7) ◽  
pp. 1019-1028 ◽  
Author(s):  
Adam J. Brown ◽  
Bernie Sweeney ◽  
David O. Mainwaring ◽  
David C. James
Keyword(s):  
Gene Expression ◽  
Cho Cells ◽  
Transient Gene Expression ◽  
Cmv Promoter

scholarly journals Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

2011 ◽  
Vol 5 (Suppl 8) ◽  
pp. P38 ◽  
Author(s):  
João Pereira ◽  
Yashas Rajendra ◽  
Lucia Baldi ◽  
David L Hacker ◽  
Florian M Wurm
Keyword(s):  
Gene Expression ◽  
Conditioned Medium ◽  
Cho Cells ◽  
Transient Gene Expression

Transient Gene Expression‐Based Protein Production in Recombinant Mammalian Cells

2019 ◽  
pp. 49-72 ◽  
Author(s):  
Joo‐Hyoung Lee ◽  
Henning G. Hansen ◽  
Sun‐Hye Park ◽  
Jong‐Ho Park ◽  
Yeon‐Gu Kim
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Protein Production ◽  
Transient Gene Expression

Rational design of transient gene expression process with lipoplexes for high-level therapeutic protein production in HEK293 cells

2019 ◽  
Vol 85 ◽  
pp. 185-194 ◽  
Author(s):  
Youngji Lee ◽  
Hyeonkyeong Kim ◽  
Eunsil Kim ◽  
Soonyong Park ◽  
Kyoung-Hwa Ryu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rational Design ◽  
Protein Production ◽  
Transient Gene Expression ◽  
Therapeutic Protein ◽  
Hek293 Cells ◽  
High Level

Investigation of relationship between EBNA-1 expression level and specific foreign protein productivity in transient gene expression of HEK293 cells

2017 ◽  
Vol 55 ◽  
pp. 182-186 ◽  
Author(s):  
Joo-Hyoung Lee ◽  
Sung-Min Lim ◽  
Sun-Hye Park ◽  
Jeong-Ki Min ◽  
Gyun Min Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression ◽  
Expression Level ◽  
Hek293 Cells ◽  
Foreign Protein ◽  
Protein Productivity
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