scholarly journals Characterization of anti-BCG benz[α]anthraquinones and new siderophores from a Xinjiang desert–isolated rare actinomycete Nocardia sp. XJ31

2020 ◽  
Vol 104 (19) ◽  
pp. 8267-8278 ◽  
Author(s):  
Li Zhang ◽  
Jingyu Zhang ◽  
Biao Ren ◽  
Wanying Lu ◽  
Chengjian Hou ◽  
...  
Keyword(s):  
Nocardia Sp

Identification and Molecular Characterization of Nocardia sp. as a New Causal Agent of Tobacco False Broomrape

2012 ◽  
Vol 161 (2) ◽  
pp. 86-91 ◽  
Author(s):  
Yunior Morán ◽  
Osmani Chacón ◽  
Maria del Carmen Córdoba-Sellés ◽  
Rosario Domínguez-Larrinaga ◽  
Lidcay Herrera ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Causal Agent ◽  
Nocardia Sp

Purification and characterization of biosurfactants from Nocardia sp. L-417

2000 ◽  
Vol 31 (3) ◽  
pp. 249 ◽  
Author(s):  
Soon Han Kim ◽  
Ee Jong Lim ◽  
Sang Ok Lee ◽  
Jae Dong Lee ◽  
Tae Ho Lee
Keyword(s):  
Purification And Characterization ◽  
Nocardia Sp

Characterization of nitrile hydratation catalysed by Nocardia sp. 108

2006 ◽  
Vol 23 (3) ◽  
pp. 355-362 ◽  
Author(s):  
Ya-Jun Wang ◽  
Yu-Guo Zheng ◽  
Jian-Ping Xue ◽  
Yin-Chu Shen
Keyword(s):  
Nocardia Sp

scholarly journals Selective isolation and characterization of rare actinomycetes adopted in glacier soil of Manali ice point and its activity against Mycobacterium spp

2017 ◽  
Vol 7 (5) ◽  
pp. 1 ◽  
Author(s):  
RAJA A ◽  
P Gajalakshmi
Keyword(s):  
Active Compound ◽  
Gradient Centrifugation ◽  
Population Isolation ◽  
Isolation And Characterization ◽  
Novel Approach ◽  
Rare Actinomycetes ◽  
Nocardia Sp ◽  
Glacier Ice ◽  
Mycobacterium Spp

An exigent demand of antimicrobial agent against M. tuberculosis was lead to the isolation of novel rare actinomycetes from the unexplored cryophilic environment. Soil samples were collected from glacier ice point of Kullu Manali and processed for further studies. A novel approach was described for the isolation of rare actinomycetes from a heterogeneous population. Isolation was done by conventional and density gradient centrifugation. Sucrose gradient centrifugation showed a maximum of 24 actinomycetes isolates which belong to the genera of Streptomyces sp (12), Micromonospora sp (5) Planomonospora sp(2), Micropolyspora sp (2), Actinopolyspora sp (1) Nocardia sp (1) and Intrasporangium sp (1). Of these 24 actinomycetes, isolate Planomonospora sp (PL-2) showed potent anti-mycobacterial activity against Mycobacterium smegmatis (MTCC300) and M.tuberculosis (MTCC 6). Bioautography reveals that the Rf value of active compound was 0.75 and retains the antimicrobial activity at 75° C. Based on the C13 and H1 NMR the active compound was characterized as 2-(2-ethenylphenyl) heptane-1-ol. Phylogenetic analysis reveals active isolate was closely related to Planomonospora alba and the Genbank accession is JQ280498.

scholarly journals Identification of Phenol-Degrading Nocardia Sp. Strain C-14-1 and Characterization of Its Ring-Cleavage 2,3-Dioxygenase

2010 ◽  
Vol 2 (1) ◽  
Author(s):  
Haijuan Ma ◽  
Guangming Li ◽  
Ping Fang ◽  
Yalei Zhang ◽  
Deqiang Xu
Keyword(s):  
Ring Cleavage ◽  
Nocardia Sp

Cloning, expression, purification, and characterization of Nocardia sp. GTP cyclohydrolase I

2004 ◽  
Vol 35 (2) ◽  
pp. 171-180 ◽  
Author(s):  
Aimin He ◽  
D.Randall Simpson ◽  
Lacy Daniels ◽  
John P.N Rosazza
Keyword(s):  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
Purification And Characterization ◽  
Nocardia Sp

scholarly journals Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family

2004 ◽  
Vol 70 (3) ◽  
pp. 1874-1881 ◽  
Author(s):  
Aimin He ◽  
Tao Li ◽  
Lacy Daniels ◽  
Ian Fotheringham ◽  
John P. N. Rosazza
Keyword(s):  
Carboxylic Acid ◽  
Gene Family ◽  
Carboxylic Acids ◽  
Affinity Tag ◽  
Aldehyde Oxidoreductase ◽  
Nocardia Sp ◽  
New Gene ◽  
Ferulic Acids

ABSTRACT We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had Km values for benzoate, ATP, and NADPH that were similar to those for natural CAR, and recombinant CAR reduced benzoic, vanillic, and ferulic acids to their corresponding aldehydes. car is the first example of a new gene family encoding oxidoreductases with remote acyl adenylation and reductase sites.

Purification and characterization of a highly selective epoxide hydrolase from Nocardia sp. EH1

1998 ◽  
Vol 61 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Wolfgang Kroutil ◽  
Yvonne Genzel ◽  
Markus Pietzsch ◽  
Christian Syldatk ◽  
Kurt Faber
Keyword(s):  
Epoxide Hydrolase ◽  
Purification And Characterization ◽  
Nocardia Sp

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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