Construction of lactic acid-tolerant Saccharomyces cerevisiae by using CRISPR-Cas-mediated genome evolution for efficient d-lactic acid production

2020 ◽  
Vol 104 (21) ◽  
pp. 9147-9158
Author(s):  
Ryosuke Mitsui ◽  
Ryosuke Yamada ◽  
Takuya Matsumoto ◽  
Shizue Yoshihara ◽  
Hayato Tokumoto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Genome Evolution ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acid Tolerant

scholarly journals Improvement of Lactic Acid Production in Saccharomyces cerevisiae by Cell Sorting for High Intracellular pH

2006 ◽  
Vol 72 (8) ◽  
pp. 5492-5499 ◽  
Author(s):  
Minoska Valli ◽  
Michael Sauer ◽  
Paola Branduardi ◽  
Nicole Borth ◽  
Danilo Porro ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Intracellular Ph ◽  
Cell Sorting ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Low Ph ◽  
The Mean ◽  
High Level ◽  
Lactate Dehydrogenases

ABSTRACT Yeast strains expressing heterologous l-lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, lactic acid exerts a high level of stress on the cells. In the present study we analyzed intracellular pH (pHi) and viability by staining with cSNARF-4F and ethidium bromide, respectively, of two lactic-acid-producing strains of Saccharomyces cerevisiae, CEN.PK m850 and CEN.PK RWB876. The results showed that the strain producing more lactic acid, CEN.PK m850, has a higher pHi. During batch culture, we observed in both strains a reduction of the mean pHi and the appearance of a subpopulation of cells with low pHi. Simultaneous analysis of pHi and viability proved that the cells with low pHi were dead. Based on the observation that the better lactic-acid-producing strain had a higher pHi and that the cells with low pHi were dead, we hypothesized that we might find better lactic acid producers by screening for cells within the highest pHi range. The screening was performed on UV-mutagenized populations through three consecutive rounds of cell sorting in which only the viable cells within the highest pHi range were selected. The results showed that lactic acid production was significantly improved in the majority of the mutants obtained compared to the parental strains. The best lactic-acid-producing strain was identified within the screening of CEN.PK m850 mutants.

Improvement of lactic acid production in Saccharomyces cerevisiae by a deletion of ssb1

2015 ◽  
Vol 43 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Jinsuk J. Lee ◽  
Nathan Crook ◽  
Jie Sun ◽  
Hal S. Alper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production

Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion

2015 ◽  
Vol 99 (19) ◽  
pp. 8023-8033 ◽  
Author(s):  
Timothy L. Turner ◽  
Guo-Chang Zhang ◽  
Soo Rin Kim ◽  
Vijay Subramaniam ◽  
David Steffen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Engineered Saccharomyces Cerevisiae

d-Lactic acid production by metabolically engineered Saccharomyces cerevisiae

2006 ◽  
Vol 101 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Nobuhiro Ishida ◽  
Tomiko Suzuki ◽  
Kenro Tokuhiro ◽  
Eiji Nagamori ◽  
Toru Onishi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Engineered Saccharomyces Cerevisiae

scholarly journals Changes in SAM 2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae

2014 ◽  
Vol 13 (1) ◽  
pp. 147 ◽  
Author(s):  
Laura Dato ◽  
Nadia Berterame ◽  
Maria Ricci ◽  
Paola Paganoni ◽  
Luigi Palmieri ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acid Tolerance

Introduction of a bacterial acetyl-CoA synthesis pathway improves lactic acid production in Saccharomyces cerevisiae

2016 ◽  
Vol 35 ◽  
pp. 38-45 ◽  
Author(s):  
Ji-Yoon Song ◽  
Joon-Song Park ◽  
Chang Duk Kang ◽  
Hwa-Young Cho ◽  
Dongsik Yang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acetyl Coa ◽  
Acetyl Coa Synthesis
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