scholarly journals Domesticating a food spoilage yeast into an organic acid‐tolerant metabolic engineering host: Lactic acid production by engineered Zygosaccharomyces bailii

2020 ◽  
Vol 118 (1) ◽  
pp. 372-382
Author(s):  
Nurzhan Kuanyshev ◽  
Christopher V. Rao ◽  
Bruce Dien ◽  
Yong‐Su Jin
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Organic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Food Spoilage ◽  
Zygosaccharomyces Bailii ◽  
Spoilage Yeast ◽  
Acid Tolerant

INFLUENCE OF FORMIC ACID AND FORMALIN ON THE PRODUCTION OF ORGANIC ACID IN DIRECT-CUT ALFALFA SILAGE

1973 ◽  
Vol 53 (1) ◽  
pp. 81-85 ◽  
Author(s):  
T. R. DAVIDSON ◽  
K. R. STEVENSON ◽  
J. BUCHANAN-SMITH
Keyword(s):  
Lactic Acid ◽  
Formic Acid ◽  
Organic Acid ◽  
Acid Production ◽  
Volatile Fatty Acids ◽  
Lactic Acid Production ◽  
Test Tube ◽  
Organic Acid Production ◽  
Subsequent Degradation ◽  
Forage Harvester

Early bloom alfalfa (Medicago sativa cult Saranac), at 22.5% dry matter, was harvested with a forage harvester. Formic acid (85% solution) and formalin (37.5% solution) and various combinations of mixtures were applied to the forage on a fresh weight basis at rates of 0.33, 0.50, and 0.66%. A sample of the treated material was ensiled in test tube silos fitted with fermentation locks. At various time intervals, analyses were made to follow the patterns of organic acid production. In untreated silage, the pH dropped to 4.3 with high lactic acid production, but after 39 days, the pH began to rise as lactic acid was degraded by Clostridia. Formic acid at 0.33 and 0.50% delayed but did not prevent either lactic acid production or subsequent degradation. Formic acid (0.66%) and all rates of formalin depressed lactic acid production. The production of butyric, isobutyric, and isovaleric acids was depressed to low levels only at the 0.66% rate of treatments. Formic acid was more effective than formalin in depressing volatile fatty acids. The formic–formalin mixtures gave results intermediate to separate applications of formic acid and formalin for all parameters analyzed.

scholarly journals Production of biopolymer precursors beta-alanine and L-lactic acid from CO2 with metabolically versatile Rhodococcus opacus DSM 43205

2020 ◽  
Author(s):  
Laura Salusjärvi ◽  
Leo Ojala ◽  
Gopal Peddinti ◽  
Michael Lienemann ◽  
Paula Jouhten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Water Electrolysis ◽  
Rhodococcus Opacus ◽  
Beta Alanine ◽  
Polymer Precursors ◽  
Autotrophic Bacteria

AbstractHydrogen oxidizing autotrophic bacteria are promising hosts for CO2 conversion into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium Rhodococcus opacus strain DSM 43205 for synthesis of polymer precursors. Aspartate decarboxylase (panD) or lactate dehydrogenase (ldh) were expressed for beta-alanine or L-lactic acid production, respectively. The heterotrophic cultivations on glucose produced 25 mg L-1 beta-alanine and 742 mg L-1 L-lactic acid, while autotrophic cultivations with CO2, H2 and O2 resulted in the production of 1.8 mg L-1 beta-alanine and 146 mg L-1 L-lactic acid. Beta-alanine was also produced at 345 µg L-1 from CO2 in electrobioreactors, where H2 and O2 were provided by water electrolysis. This work demonstrates that R. opacus DSM 43205 can be readily engineered to produce chemicals from CO2 and provides base for its further metabolic engineering.

Metabolic engineering of Pichia pastoris for L-lactic acid production using glycerin as carbon source

2015 ◽  
Author(s):  
Nadia Skorupa Parachin ◽  
Pollyne Lima ◽  
Nadielle Melo ◽  
Lucas Carvalho ◽  
Virgililio Castro ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Pichia Pastoris ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production

Construction of lactic acid-tolerant Saccharomyces cerevisiae by using CRISPR-Cas-mediated genome evolution for efficient d-lactic acid production

2020 ◽  
Vol 104 (21) ◽  
pp. 9147-9158
Author(s):  
Ryosuke Mitsui ◽  
Ryosuke Yamada ◽  
Takuya Matsumoto ◽  
Shizue Yoshihara ◽  
Hayato Tokumoto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Genome Evolution ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acid Tolerant

Application of acid tolerant Pedioccocus strains for increasing the sustainability of lactic acid production from cheese whey

LWT ◽  
2016 ◽  
Vol 72 ◽  
pp. 399-406 ◽  
Author(s):  
Grazina Juodeikiene ◽  
Daiva Zadeike ◽  
Elena Bartkiene ◽  
Dovile Klupsaite
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Cheese Whey ◽  
Lactic Acid Production ◽  
Acid Tolerant

Improvement of d ‐Lactic Acid Production in Saccharomyces cerevisiae Under Acidic Conditions by Evolutionary and Rational Metabolic Engineering

2017 ◽  
Vol 12 (10) ◽  
pp. 1700015 ◽  
Author(s):  
Seung‐Ho Baek ◽  
Eunice Y. Kwon ◽  
Sang‐Jeong Bae ◽  
Bo‐Ram Cho ◽  
Seon‐Young Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acidic Conditions

Metabolic engineering as a tool for enhanced lactic acid production

2014 ◽  
Vol 32 (12) ◽  
pp. 637-644 ◽  
Author(s):  
Bikram P. Upadhyaya ◽  
Linda C. DeVeaux ◽  
Lew P. Christopher
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production

Metabolic engineering approaches for lactic acid production

2006 ◽  
Vol 41 (5) ◽  
pp. 991-1000 ◽  
Author(s):  
Sudheer K. Singh ◽  
Syed U. Ahmed ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production
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