New insights into the biology of multiple myeloma using a combination of May-Grünwald-Giemsa staining and fluorescence in situ hybridization techniques at the single cell level

2001 ◽  
Vol 80 (11) ◽  
pp. 662-668 ◽  
Author(s):  
Nickenig C. ◽  
Lang N. ◽  
Schoch C. ◽  
Hiddemann W. ◽  
Haferlach T.
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Giemsa Staining ◽  
Single Cell Level ◽  
Cell Level

scholarly journals A molecular pathology method for sequential fluorescence in situ hybridization for multi-gene analysis at the single-cell level

Oncotarget ◽  
2016 ◽  
Vol 8 (31) ◽  
pp. 50534-50541
Author(s):  
Linping Hu ◽  
Xiuxiu Yin ◽  
Jiangman Sun ◽  
Anders Zetterberg ◽  
Weimin Miao ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Molecular Pathology ◽  
Gene Analysis ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Evaluation of the Redox Dye 5-Cyano-2,3-Tolyl-Tetrazolium Chloride for Activity Studies by Simultaneous Use of Microautoradiography and Fluorescence In Situ Hybridization

2003 ◽  
Vol 69 (1) ◽  
pp. 641-643 ◽  
Author(s):  
Jeppe Lund Nielsen ◽  
Marilena Aquino de Muro ◽  
Per Halkjær Nielsen
Keyword(s):  
In Situ Hybridization ◽  
Activated Sludge ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Single Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Tetrazolium Chloride ◽  
In Situ Techniques

ABSTRACT Three microscopic in situ techniques were used simultaneously to investigate viability and activity on a single-cell level in activated sludge. The redox dye 5-cyano-2,3-tolyl-tetrazolium chloride (CTC) was compared with microautoradiography (MAR) and fluorescence in situ hybridization (FISH) to indicate activity of cells in Thiothrix filaments and in single floc-forming bacteria. The signals from MAR and FISH correlated well, whereas only 65% of the active Thiothrix cells and 41% of all single cells were detectable by CTC reduction, which mainly targeted the most active cells.

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