Evaluation of the Redox Dye 5-Cyano-2,3-Tolyl-Tetrazolium Chloride for Activity Studies by Simultaneous Use of Microautoradiography and Fluorescence In Situ Hybridization
2003 ◽
Vol 69
(1)
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pp. 641-643
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Keyword(s):
ABSTRACT Three microscopic in situ techniques were used simultaneously to investigate viability and activity on a single-cell level in activated sludge. The redox dye 5-cyano-2,3-tolyl-tetrazolium chloride (CTC) was compared with microautoradiography (MAR) and fluorescence in situ hybridization (FISH) to indicate activity of cells in Thiothrix filaments and in single floc-forming bacteria. The signals from MAR and FISH correlated well, whereas only 65% of the active Thiothrix cells and 41% of all single cells were detectable by CTC reduction, which mainly targeted the most active cells.
1997 ◽
Vol 97
(1)
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pp. 99-106
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2001 ◽
Vol 81
(10)
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pp. 1457-1459
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1997 ◽
Vol 99
(2)
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pp. 452-459
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