Faculty Opinions recommendation of Linking microbial phylogeny to metabolic activity at the single-cell level by using enhanced element labeling-catalyzed reporter deposition fluorescence in situ hybridization (EL-FISH) and NanoSIMS.

2008 ◽  
Author(s):  
Michael Wagner
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Metabolic Activity ◽  
Single Cell Level ◽  
Cell Level ◽  
Catalyzed Reporter Deposition

scholarly journals A molecular pathology method for sequential fluorescence in situ hybridization for multi-gene analysis at the single-cell level

Oncotarget ◽  
2016 ◽  
Vol 8 (31) ◽  
pp. 50534-50541
Author(s):  
Linping Hu ◽  
Xiuxiu Yin ◽  
Jiangman Sun ◽  
Anders Zetterberg ◽  
Weimin Miao ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Molecular Pathology ◽  
Gene Analysis ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Evaluation of the Redox Dye 5-Cyano-2,3-Tolyl-Tetrazolium Chloride for Activity Studies by Simultaneous Use of Microautoradiography and Fluorescence In Situ Hybridization

2003 ◽  
Vol 69 (1) ◽  
pp. 641-643 ◽  
Author(s):  
Jeppe Lund Nielsen ◽  
Marilena Aquino de Muro ◽  
Per Halkjær Nielsen
Keyword(s):  
In Situ Hybridization ◽  
Activated Sludge ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Single Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Tetrazolium Chloride ◽  
In Situ Techniques

ABSTRACT Three microscopic in situ techniques were used simultaneously to investigate viability and activity on a single-cell level in activated sludge. The redox dye 5-cyano-2,3-tolyl-tetrazolium chloride (CTC) was compared with microautoradiography (MAR) and fluorescence in situ hybridization (FISH) to indicate activity of cells in Thiothrix filaments and in single floc-forming bacteria. The signals from MAR and FISH correlated well, whereas only 65% of the active Thiothrix cells and 41% of all single cells were detectable by CTC reduction, which mainly targeted the most active cells.

Sign in / Sign up

Export Citation Format

Share Document