Fish otolith geochemistry, environmental conditions and human occupation at Lake Mungo, Australia

Fish otoliths from the Willandra Lakes Region World Heritage Area (south-western New South Wales, Australia) have been analysed for oxygen isotopes and trace elements using in situ techniques, and dated by radiocarbon. The study focused on the lunettes of Lake Mungo, an overflow lake that only filled during flooding events and emptied by evaporation, and Lake Mulurulu, which was part of the running Willandra Creek system. Samples were collected from two different contexts: from hearths directly associated with human activity, and isolated surface finds. AMS radiocarbon dating constrains the human activity documented by five different hearths to a time span of less than 240 years around 19,350 cal. BP. These hearths were constructed in aeolian sediments with alternating clay and sand layers, indicative of fluctuating lake levels and occasional drying out. The geochemistry of the otoliths confirms this scenario, with shifts in Sr/Ca and Ba/Ca marking the entry of the fish into Lake Mungo several years before their death, and a subsequent increase in the δ18O by ∼4‰ indicating increasing evaporation of the lake. During sustained lake-full conditions there are considerably fewer traces of human presence. It seems that the evaporating Lake Mungo attracted people to harvest fish that might have become sluggish through oxygen starvation in an increasingly saline water body (easy prey hypothesis). In contrast, surface finds have a much wider range in radiocarbon age as a result of reworking, and do not necessarily indicate evaporative conditions, as shown by comparison with otoliths from upstream Lake Mulurulu.

In Situ and In Operando Techniques to Study Li-Ion and Solid-State Batteries: Micro to Atomic Level

This work summarizes the most commonly used in situ techniques for the study of Li-ion batteries from the micro to the atomic level. In situ analysis has attracted a great deal of interest owing to its ability to provide a wide range of information about the cycling behavior of batteries from the beginning until the end of cycling. The in situ techniques that are covered are: X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Scanning Transmission Electron Microscopy (STEM). An optimized setup is required to be able to use any of these in situ techniques in battery applications. Depending on the type of data required, the available setup, and the type of battery, more than one of these techniques might be needed. This study organizes these techniques from the micro to the atomic level, and shows the types of data that can be obtained using these techniques, their advantages and their challenges, and possible strategies for overcoming these challenges.

Jet energy scale and resolution measured in proton–proton collisions at $$\sqrt{s}=13$$ TeV with the ATLAS detector

Jet energy scale and resolution measurements with their associated uncertainties are reported for jets using 36–81 fb$$^{-1}$$ - 1 of proton–proton collision data with a centre-of-mass energy of $$\sqrt{s}=13$$ s = 13 $${\text {Te}}{\text {V}}$$ TeV collected by the ATLAS detector at the LHC. Jets are reconstructed using two different input types: topo-clusters formed from energy deposits in calorimeter cells, as well as an algorithmic combination of charged-particle tracks with those topo-clusters, referred to as the ATLAS particle-flow reconstruction method. The anti-$$k_t$$ k t jet algorithm with radius parameter $$R=0.4$$ R = 0.4 is the primary jet definition used for both jet types. This result presents new jet energy scale and resolution measurements in the high pile-up conditions of late LHC Run 2 as well as a full calibration of particle-flow jets in ATLAS. Jets are initially calibrated using a sequence of simulation-based corrections. Next, several in situ techniques are employed to correct for differences between data and simulation and to measure the resolution of jets. The systematic uncertainties in the jet energy scale for central jets ($$|\eta |<1.2$$ | η | < 1.2 ) vary from 1% for a wide range of high-$$p_{{\text {T}}}$$ p T jets ($$250<p_{{\text {T}}} <2000~{\text {Ge}}{\text {V}}$$ 250 < p T < 2000 GeV ), to 5% at very low $$p_{{\text {T}}}$$ p T ($$20~{\text {Ge}}{\text {V}}$$ 20 GeV ) and 3.5% at very high $$p_{{\text {T}}}$$ p T ($$>2.5~{\text {Te}}{\text {V}}$$ > 2.5 TeV ). The relative jet energy resolution is measured and ranges from ($$24 \pm 1.5$$ 24 ± 1.5 )% at 20 $${\text {Ge}}{\text {V}}$$ GeV to ($$6 \pm 0.5$$ 6 ± 0.5 )% at 300 $${\text {Ge}}{\text {V}}$$ GeV .

MODEL PARAMETERS FOR THE DYNAMIC BEHAVIOR OF BURIED PIPES-PART 1

Pipeline systems are an integral part of modern human activity for much of the accompanying processes of lifestyle and industry. The design and construction of underground facilities solves one major problem-improving the quality of life by deploying the systems near the consumer, without intruding on the human environment. The necessity for more efficient behavior prognosis and evaluation as well as better design of buried pipelines during and after various actions (including geologic and seismic hazards) is directly related to the knowledge of their working mechanism, while aiming to prevent incidents which impact the human life. A number of additional parameters describing the conditions, behavior and effects of the pipeline system, going beyond the conventional Eurocode methods, is a requirement for the scope of this study. This study covers a comprehensive research system based on laboratory and in situ techniques, numerical analyses and the creation of interaction parameters of a buried pipe with soil massif in dynamic situations. Laboratory analyses were conducted in a laboratory triaxial state with cyclic shear strains. All parameters that were defined by constitution models-Mohr-Coulomb, Hardening soil model, Modified Cam-Clay based in a Plaxis software were traced. In the final phase of the publication (Part 2) the implementation and evaluation of the formulated parameters and results of the study are demonstrated.

Selected In Situ Hybridization Methods: Principles and Application

We are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complementary bind is one of the main principles in every method used in ISH. From the first use of in situ techniques, scientists paid attention to the improvement of the probe design and detection, to enhance the fluorescent signal intensity and inhibition of cross-hybrid presence. This article discusses the individual types and modifications, and is focused on explaining the principles and limitations of ISH division on different types of probes. The article describes a design of probes for individual types of in situ hybridization (ISH), as well as the gradual combination of several laboratory procedures to achieve the highest possible sensitivity and to prevent undesirable events accompanying hybridization. The article also informs about applications of the methodology, in practice and in research, to detect cell to cell communication and principles of gene silencing, process of oncogenesis, and many other unknown processes taking place in organisms at the DNA/RNA level.

Hydrogen spillover-driven synthesis of high-entropy alloy nanoparticles as a robust catalyst for CO2 hydrogenation

High-entropy alloys (HEAs) have been intensively pursued as potentially advanced materials because of their exceptional properties. However, the facile fabrication of nanometer-sized HEAs over conventional catalyst supports remains challenging, and the design of rational synthetic protocols would permit the development of innovative catalysts with a wide range of potential compositions. Herein, we demonstrate that titanium dioxide (TiO2) is a promising platform for the low-temperature synthesis of supported CoNiCuRuPd HEA nanoparticles (NPs) at 400 °C. This process is driven by the pronounced hydrogen spillover effect on TiO2 in conjunction with coupled proton/electron transfer. The CoNiCuRuPd HEA NPs on TiO2 produced in this work were found to be both active and extremely durable during the CO2 hydrogenation reaction. Characterization by means of various in situ techniques and theoretical calculations elucidated that cocktail effect and sluggish diffusion originating from the synergistic effect obtained by this combination of elements.

Obtaining Cocrystals by Reaction Crystallization Method: Pharmaceutical Applications

Cocrystals have gained attention in the pharmaceutical industry due to their ability to improve solubility, stability, in vitro dissolution rate, and bioavailability of poorly soluble drugs. Conceptually, cocrystals are multicomponent solids that contain two or more neutral molecules in stoichiometric amounts within the same crystal lattice. There are several techniques for obtaining cocrystals described in the literature; however, the focus of this article is the Reaction Crystallization Method (RCM). This method is based on the generation of a supersaturated solution with respect to the cocrystal, while this same solution is saturated or unsaturated with respect to the components of the cocrystal individually. The advantages of the RCM compared with other cocrystallization techniques include the ability to form cocrystals without crystallization of individual components, applicability to the development of in situ techniques for the screening of high quality cocrystals, possibility of large-scale production, and lower cost in both time and materials. An increasing number of scientific studies have demonstrated the use of RCM to synthesize cocrystals, mainly for drugs belonging to class II of the Biopharmaceutics Classification System. The promising results obtained by RCM have demonstrated the applicability of the method for obtaining pharmaceutical cocrystals that improve the biopharmaceutical characteristics of drugs.