Gene Cloning and Expression of an Alkaline Serine Protease with Dehairing Function from Bacillus pumilus

2004 ◽  
Vol 49 (3) ◽  
Author(s):  
Jiao Pan ◽  
Qing Huang ◽  
YiZheng Zhang
Keyword(s):  
Gene Cloning ◽  
Serine Protease ◽  
Bacillus Pumilus ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Alkaline Serine Protease

scholarly journals High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chang Chang ◽  
Siyi Gong ◽  
Zhiping Liu ◽  
Qiaojuan Yan ◽  
Zhengqiang Jiang
Keyword(s):  
Serine Protease ◽  
Whey Protein ◽  
Sodium Dodecyl ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Protease Gene ◽  
Potential Candidate ◽  
Geobacillus Stearothermophilus ◽  
Alkaline Serine Protease ◽  
Hydrolysis Of

Abstract Background Proteases are important for hydrolysis of proteins to generate peptides with many bioactivities. Thus, the development of novel proteases with high activities is meaningful to discover bioactive peptides. Because natural isolation from animal, plant and microbial sources is impractical to produce large quantities of proteases, gene cloning and expression of target protease are preferred. Results In this study, an alkaline serine protease gene (GsProS8) from Geobacillus stearothermophilus was successfully cloned and expressed in Bacillus subtilis. The recombinant GsProS8 was produced with high protease activity of 3807 U/mL after high cell density fermentation. GsProS8 was then purified through ammonium sulfate precipitation and a two-step chromatographic method to obtain the homogeneous protease. The molecular mass of GsProS8 was estimated to be 27.2 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and 28.3 kDa by gel filtration. The optimal activity of GsProS8 was found to be pH 8.5 and 50 °C, respectively. The protease exhibited a broad substrate specificity and different kinetic parameters to casein and whey protein. Furthermore, the hydrolysis of whey protein using GsProS8 resulted in a large amount of peptides with high angiotensin-I-converting enzyme (ACE) inhibitory activity (IC50 of 0.129 mg/mL). Conclusions GsProS8 could be a potential candidate for industrial applications, especially the preparation of antihypertensive peptides.

scholarly journals A novel chitosanase from Bacillus sp. Strain K17: Gene cloning and expression in Escherichia coli

2002 ◽  
Vol 2 (1) ◽  
pp. 227-228 ◽  
Author(s):  
R. Yatsunami ◽  
Y. Sakihama ◽  
M. Suzuki ◽  
T. Fukazawa ◽  
S. Shimizu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cloning ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Bacillus Sp ◽  
Expression In Escherichia Coli

Separation and identification of Brassica rapa BrAFP and its gene cloning and expression under freezing stress

2019 ◽  
Vol 138 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Zigang Liu ◽  
Xiaoyun Dong ◽  
Li Ma ◽  
Wancang Sun ◽  
Gang Yang ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Brassica Rapa ◽  
Cloning And Expression ◽  
Freezing Stress ◽  
Gene Cloning And Expression ◽  
Its Gene
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