Recombinant mitochondrial DNA molecules suggest a template switching ability for group-II-intron reverse transcriptase

2000 ◽  
Vol 37 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Carole H. Sellem ◽  
Odile Begel ◽  
Annie Sainsard-Chanet
Keyword(s):  
Mitochondrial Dna ◽  
Reverse Transcriptase ◽  
Group Ii Intron ◽  
Template Switching ◽  
Dna Molecules ◽  
Group Ii

scholarly journals Structural basis for template switching by a group II intron-encoded non-LTR-retroelement reverse transcriptase

2021 ◽  
pp. 100971
Author(s):  
Alfred M. Lentzsch ◽  
Jennifer L. Stamos ◽  
Jun Yao ◽  
Rick Russell ◽  
Alan M. Lambowitz
Keyword(s):  
Reverse Transcriptase ◽  
Group Ii Intron ◽  
Structural Basis ◽  
Template Switching ◽  
Group Ii

scholarly journals Group II intron domain 5 facilitates a trans-splicing reaction.

1988 ◽  
Vol 8 (6) ◽  
pp. 2361-2366 ◽  
Author(s):  
K A Jarrell ◽  
R C Dietrich ◽  
P S Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Intron Sequence ◽  
Trans Splicing ◽  
Group Ii ◽  
Self Splicing ◽  
Yeast Mitochondrial Dna ◽  
Domain 4

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

scholarly journals Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase

2017 ◽  
Vol 46 (1) ◽  
pp. e1-e1 ◽  
Author(s):  
Samuel T Carrell ◽  
Zhenzhi Tang ◽  
Sabine Mohr ◽  
Alan M Lambowitz ◽  
Charles A Thornton
Keyword(s):  
Reverse Transcriptase ◽  
Group Ii Intron ◽  
Group Ii
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