Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

2005 ◽  
Vol 25 (4) ◽  
pp. 257-264 ◽  
Author(s):  
L. Cistué ◽  
M. Soriano ◽  
A. M. Castillo ◽  
M. P. Vallés ◽  
J. M. Sanz ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Turgidum ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Isolated Microspore Culture

scholarly journals Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 101-109 ◽  
Author(s):  
Olfa Slama-Ayed ◽  
Imen Bouhaouel ◽  
Sourour Ayed ◽  
Jacques De Buyser ◽  
Emmanuel Picard ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Turgidum ◽  
Microspore Culture ◽  
Plant Production ◽  
Haploid Plant ◽  
Interspecific Crosses ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Good Ability ◽  
Successful Approach

This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.  

Isolated microspore cultures of a Hungarian durum wheat ( Triticum turgidum L.) cultivar, Martondur 1

2007 ◽  
Vol 55 (2) ◽  
pp. 157-164 ◽  
Author(s):  
F. Bakos ◽  
A. Fábián ◽  
B. Barnabás
Keyword(s):  
High Temperature ◽  
Durum Wheat ◽  
Triticum Turgidum ◽  
Microspore Culture ◽  
Cold Treatment ◽  
Microspore Embryogenesis ◽  
Isolated Microspore Culture ◽  
High Positive Correlation ◽  
Starting Point ◽  
Very High

A number of sporophytically induced microspores and embryo-like structures (ELS) were obtained from isolated microspore cultures of durum wheat ( Triticum turgidum L. cv. Martondur 1). Various pre-treatments were screened, involving spike treatment at 4°C for 2, 7 or 14 days; anther treatment in 0.4 M mannitol containing macroelements at 33°C for 3 days, and various combinations of these. The frequency of embryogenic (star-like) microspores and the number of ELS showed a very high positive correlation in the cultures. Starvation at high temperature was necessary to achieve a reasonable frequency of microspore embryogenesis. The best results were achieved when starvation at high temperature was combined with no or short (2-day) cold treatment (212±77 and 203±34 ELS/100 anthers, respectively). However, the ELS failed to regenerate; only a few of them produced poorly-developed albino shoots. The present work could be a promising starting point for the production of doubled haploid durum wheat plants in Hungary via isolated microspore culture.

scholarly journals Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1950
Author(s):  
Anna Mineykina ◽  
Ludmila Bondareva ◽  
Alexey Soldatenko ◽  
Elena Domblides
Keyword(s):  
Culture Medium ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Antioxidant Properties ◽  
F1 Hybrids ◽  
Anthocyanin Content ◽  
Isolated Microspore Culture ◽  
Culture In Vitro ◽  
Red Cabbage

Red cabbage belongs to the economically important group of vegetable crops of the Brassicaceae family. A unique feature of this vegetable crop that distinguishes it from other members of the family is its unique biochemical composition characterized by high anthocyanin content, which gives it antioxidant properties. The production mainly uses F1 hybrids, which require constant parental lines, requiring 6–7 generations of inbreeding. Culture of isolated microspores in vitro is currently one of the promising methods for the accelerated production of pure lines with 100% homozygosity. The aim of this study is to investigate the factors and select optimal parameters for successful induction of red cabbage embryogenesis in isolated microspore culture in vitro and subsequent regeneration of DH plants. As a result of research, for the first time, it was possible to carry out the full cycle of obtaining DH plants of red cabbage from the induction of embryogenesis to their inclusion in the breeding process. The size of buds containing predominantly microspores at the late vacuolated stage and pollen at the early bi-cellular stage has to be selected individually for each genotype, because the embryoid yield will be determined by the interaction of these two factors. In the six samples studied, the maximum embryoid yield was obtained from buds 4.1–4.4 mm and 4.5–5.0 mm long, depending on the genotype. Cultivation of microspores was carried out on liquid NLN culture medium with 13% sucrose. The maximum number of embryoids (173.5 ± 7.5 pcs./Petri dish) was obtained on culture medium with pH 5.8 and heat shock at 32 °C for 48 h. Successful embryoid development and plant regeneration by direct germination from shoot apical meristem were achieved on MS culture medium with 2% sucrose and 0.7% agar, supplemented with 6-benzylaminopurine at a concentration of 1 mg/L. Analysis of the obtained regenerated plants, which successfully passed the stage of adaptation to ex vitro conditions by flow cytometry, showed that most of them were doubled haploids (up to 90.9%). A low number of seeds produced by self-fertilization in DH plants was observed.

scholarly journals Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L.

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 278 ◽  
Author(s):  
Daria Shumilina ◽  
Dmitry Kornyukhin ◽  
Elena Domblides ◽  
Alexey Soldatenko ◽  
Anna Artemyeva
Keyword(s):  
Microspore Culture ◽  
Doubled Haploids ◽  
Genetic Disorder ◽  
Cold Treatment ◽  
Microspore Embryogenesis ◽  
Culture Conditions ◽  
Secondary Embryogenesis ◽  
Isolated Microspore Culture ◽  
Pure Lines

Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-consuming process. The production of pure turnip lines using doubled haploids produced in isolated microspore culture has not been sufficiently developed. The aim of the present work was to determine some key factors inducing embryogenesis in the isolated microspore culture of turnip, as well as investigating the manners of embryo development. It was shown that the acidity of the medium is an important factor in embryo production; different optimal pH levels ranging from 6.2 to 6.6 corresponded to individual genotypes. Such factors as the cold treatment of buds and the addition of activated charcoal to the nutrient medium increased the responsiveness of all genotypes studied. The turnip variety ‘Ronde witte roodkop herfst’ demonstrated a genetic disorder in the development of microspores; namely, non-separation of some microspores from tetrads. In the in vitro culture, each of the daughter microspores developed on its own. This indicates the dependence of the possibility of embryogenesis in the turnip microspore culture on the genotype. Results suggest that the initiation of secondary embryogenesis in primary embryos leads to an increase in the proportion of doubled haploid plants.

scholarly journals The effect of copper on plant regeneration in barley microspore culture

2017 ◽  
Vol 53 (No. 1) ◽  
pp. 17-22 ◽  
Author(s):  
K. Makowska ◽  
S. Oleszczuk ◽  
J. Zimny
Keyword(s):  
Plant Regeneration ◽  
Copper Sulphate ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Spring Barley ◽  
Culture Method ◽  
Induction Medium ◽  
Isolated Microspore Culture ◽  
Albino Plants ◽  
Effect Of Copper

Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture.

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