Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N6-(Δ2-isopentenyl)-adenine (iP) and N6-(Δ2-isopentenyl)-adenosine (iPA) levels

2008 ◽  
Vol 27 (8) ◽  
pp. 1281-1289 ◽  
Author(s):  
Guek Eng Sim ◽  
Chong Jin Goh ◽  
Chiang Shiong Loh
Keyword(s):  
In Vitro Flowering ◽  
Isopentenyl Adenine ◽  
Isopentenyl Adenosine

In vitro flowering and fruiting in Portulaca pilosa L.

2021 ◽  
Vol 140 ◽  
pp. 1-3
Author(s):  
Yuping Xiong ◽  
Shuangyan Chen ◽  
Zhenpeng Wei ◽  
Xincheng Yu ◽  
Jinhui Pang ◽  
...  
Keyword(s):  
In Vitro Flowering

scholarly journals Rejuvenation of chicory and lettuce plants following phase change in tissue culture

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Anthony J. Conner ◽  
Helen Searle ◽  
Jeanne M. E. Jacobs
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Seed Set ◽  
Genetic Manipulation ◽  
Adventitious Shoot ◽  
In Vitro Flowering ◽  
Phase Changes ◽  
Adventitious Shoot Regeneration ◽  
Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

Influence of preculture treatment and types of explants on shoot growth and in vitro flowering of feathered amaranth (Celosia argentea var. plumose)

2010 ◽  
Vol 105 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Kitti Bodhipadma ◽  
Sompoch Noichinda ◽  
Winan Padyencheun ◽  
Theerapong Khunthacharoen ◽  
Utorn Chikhunthod ◽  
...  
Keyword(s):  
Shoot Growth ◽  
In Vitro Flowering ◽  
Celosia Argentea

scholarly journals In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

2011 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Suttinee JINGJIT ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
In Vitro Flowering ◽  
Nodal Explants ◽  
Callus Growth ◽  
Ms Medium ◽  
Old Field ◽  
Gypsophila Paniculata ◽  
Initial Culture

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

scholarly journals AgNO3 improved micropropagation and stimulate in vitro flowering of rose (Rosa x hybrida) cv. Sena

2021 ◽  
Vol 27 (1) ◽  
pp. 33-40
Author(s):  
Ana Victória Conde da Silva de Matos ◽  
Bárbara Samantha de Oliveira ◽  
Maria Eduarda Barboza Souza de Oliveira ◽  
Jean Carlos Cardoso
Keyword(s):  
In Vitro Flowering ◽  
Light Sources ◽  
Multiplication Rate ◽  
Cut Flower ◽  
Physiological Disorders ◽  
Fluorescent Lamps ◽  
The Rose ◽  
Disease Free ◽  
Early Leaf Senescence

Abstract Rose is one of the most important cut flower in the world. Rose micropropagation was used for production of clonal and disease-free plantlets and to breeding purposes. However, many important rose cultivars showed physiological disorders as early-leaf senescence and very low multiplication rate under in vitro conditions. Our hypothesis is that these symptoms were associated with high sensibility of these cultivars to ethylene accumulation on in vitro environment. The rose cv. Sena was in vitro cultivated under different concentrations of AgNO3 and two light sources, LED and fluorescent lamps, as a way to investigate in vitro similar symptoms to ethylene accumulation. AgNO3 at 1.0-2.0 mg L-1 solved the main in vitro physiological disorders observed in this rose cultivar. Also, AgNO3 stimulated induction of 50% of rose shoots to in vitro flowering at 2.0 mg L-1. Higher concentrations also resulted in flowering induction, but with imperfect flower development.

In Vitro Flowering

2011 ◽  
pp. 106-127
Author(s):  
Ralph Scorza
Keyword(s):  
In Vitro Flowering
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