scholarly journals AgNO3 improved micropropagation and stimulate in vitro flowering of rose (Rosa x hybrida) cv. Sena

2021 ◽  
Vol 27 (1) ◽  
pp. 33-40
Author(s):  
Ana Victória Conde da Silva de Matos ◽  
Bárbara Samantha de Oliveira ◽  
Maria Eduarda Barboza Souza de Oliveira ◽  
Jean Carlos Cardoso
Keyword(s):  
In Vitro Flowering ◽  
Light Sources ◽  
Multiplication Rate ◽  
Cut Flower ◽  
Physiological Disorders ◽  
Fluorescent Lamps ◽  
The Rose ◽  
Disease Free ◽  
Early Leaf Senescence

Abstract Rose is one of the most important cut flower in the world. Rose micropropagation was used for production of clonal and disease-free plantlets and to breeding purposes. However, many important rose cultivars showed physiological disorders as early-leaf senescence and very low multiplication rate under in vitro conditions. Our hypothesis is that these symptoms were associated with high sensibility of these cultivars to ethylene accumulation on in vitro environment. The rose cv. Sena was in vitro cultivated under different concentrations of AgNO3 and two light sources, LED and fluorescent lamps, as a way to investigate in vitro similar symptoms to ethylene accumulation. AgNO3 at 1.0-2.0 mg L-1 solved the main in vitro physiological disorders observed in this rose cultivar. Also, AgNO3 stimulated induction of 50% of rose shoots to in vitro flowering at 2.0 mg L-1. Higher concentrations also resulted in flowering induction, but with imperfect flower development.

scholarly journals IN VITRO DEVELOPMENT OF YELLOW LAPACHO (BIGNONIACEAE) USING HIGH-POWER LIGHT EMITTING DIODE

2018 ◽  
Vol 42 (5) ◽  
Author(s):  
Ezequiel Enrique Larraburu ◽  
Gonzalo Sanchez Correa ◽  
Berta Elizabet Llorente
Keyword(s):  
In Vitro Culture ◽  
High Power ◽  
Stomatal Density ◽  
Light Emitting Diode ◽  
Dry Weight ◽  
Light Sources ◽  
Multiplication Rate ◽  
Light Emitting ◽  
Shoot Dry Weight

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

scholarly journals In vitro Regeneration and Callogenesis of Libidibia ferrea

2020 ◽  
pp. 14-24
Author(s):  
Daniel da Silva ◽  
Angela Maria Imakawa ◽  
Kamylla Rosas Vieira Guedes ◽  
Flávio Mauro Souza Bruno ◽  
Paulo de Tarso Barbosa Sampaio
Keyword(s):  
Culture Media ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Light Sources ◽  
Multiplication Rate ◽  
Medicinal Species ◽  
Efficient System ◽  
Blue Led ◽  
Friable Callus

Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

scholarly journals Light sources on the germination and initial in vitro establishment of Schomburgkia crispa Lindl., a species of the Brazilian Cerrado

2021 ◽  
Vol 51 (3) ◽  
Author(s):  
José Carlos Sorgato ◽  
Erick Dutra Mudolon ◽  
Fernando Figueiredo Guimarães ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Germination Percentage ◽  
In Vitro Cultivation ◽  
Light Sources ◽  
White Led ◽  
Brazilian Cerrado ◽  
Seedling Mortality ◽  
Fluorescent Lamps ◽  
Growth Room ◽  
In Vitro Establishment

ABSTRACT: Light is one of the factors that influence the germination and initial establishment of orchids under in vitro cultivation. This study evaluated the effect of different light sources on these stages in in vitro cultivation of Schomburgkia crispa Lindl. After sowing in an aseptic environment, we stored the cultures in a screened greenhouse (natural light) or in a growth room with the following light sources: 3,000 K yellow LED; 6,500 K white LED [1]; 6,500 K white LED [2]; or 6,500 K white fluorescent lamp (control). We assessed germination percentage and initial seedling establishment at 45 and 90 days after sowing. Light did not influence the germination of S. crispa. However, the use of 3,000 K LED provided a faster initial establishment of S. crispa when compared to the other light sources, also presenting lower seedling mortality. Thus, the light source 3,000 K LED is a potential substitute for the 6,500 K fluorescent lamps and LEDs used in growth rooms in in vitro culture laboratories.

scholarly journals Optimising Gelling Agents, Light Source and After-care to Commercialise Teak Tissue Culture

2018 ◽  
Vol 28 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Ramasamy Mahalakshmi ◽  
Manikantan Vijayamma Vineetha ◽  
Rekha Ravindranath Warrier
Keyword(s):  
Shoot Multiplication ◽  
Tectona Grandis ◽  
Multiple Shoot ◽  
Steady Increase ◽  
Light Sources ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Gelling Agents ◽  
Significant Difference

Mass propagation of Teak (Tectona grandis Linn. f.) a commercial species is a priority to increase multiplication rate and meet the growing demand for planting material. The present study has assessed the in vitro performance of Teak Tissue Cultures with different gelling agents, different light conditions, and aftercare of ex vitro rooted shoots to enhance their survival rate. Multiple shoot formation was induced from excised seedling nodal explants on MS supplemented with BA and Kn and about 5 - 10 shoots were obtained from each explant. Significant variation (p > 0.05) was observed in the number of shoots produced by the different clones. Three gelling agents (agar, phytagel and gellan gum), and two light sources (tube light and LED) were tested for enhancing shoot multiplication. No significant difference in in vitro growth was observed between clones with different solidifying agents. Teak, however, did not respond favourably to LED lights. Rooting-acclimatization phase was achieved in the nursery with 80 - 95 per cent success. The rooted plants were sprayed with DAP and Humaur to assess the growth performance following transplanting. Significant variations in rooting indicate the existence of physiological variations among the clones. Application of fertilizers promoted an initial boost followed by a steady increase during the rest of the study period. Clones with high multiplication rates under in vitro conditions could be selected for commercialization of teak multiplication. Under ex vitro conditions, a spray of fertilizers during the initial establishment phase would result in increased vigour of transplantable plants. This would ensure better survival on out planting.Plant Tissue Cult. & Biotech. 28(1): 13-24, 2018 (June)

scholarly journals Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration

2013 ◽  
Vol 43 (7) ◽  
pp. 1168-1173 ◽  
Author(s):  
Paulo Sérgio Gomes da Rocha ◽  
Roberto Pedroso de Oliveira ◽  
Walkyria Bueno Scivittaro
Keyword(s):  
Light Source ◽  
Growth Medium ◽  
Light Emitting Diodes ◽  
Sucrose Concentration ◽  
Saccharum Officinarum ◽  
Growth Media ◽  
Light Sources ◽  
Multiplication Rate ◽  
Light Emitting ◽  
Fluorescent Lamps

The aim of this research was to evaluate the use of light emitting diodes (LEDs) instead of white fluorescent lamps as light source and adequate growth-medium sucrose concentration for sugarcane micropropagation (Saccharum officinarum L.). Sugarcane (RB 872552 variety) bud explants were evaluated during the multiplication and rooting phases under controlled growth-room conditions. Different light sources (blue, red and green LEDs; Growlux and white fluorescent lamps) and different medium sucrose concentrations (0; 15; 30 and 45g L-1) were used, maintaining constant light intensity (20µmol m-2 s-1), photoperiod (16h) and temperature (25+2°C). The experiment was a completely randomized design, and treatments were arranged in a 5x4 factorial (five light sources and four medium sucrose concentrations) with six replications. Sugarcane bud growth was satisfactory under the three LED types studied. The presence of sucrose in growth media was essential for bud multiplication and rooting. Nevertheless, each light source requires the respective medium sucrose concentration adjustment for best results. Red LEDs provided a significantly high multiplication rate (although not the highest) with 8.5 buds per sub-culture and 34.9g L-1 of sucrose; also, the highest bud length (33.3mm) and the best plantlet acclimatization. Therefore, LED sources can advantageously substitute fluorescent lamps in laboratories of sugarcane micropropagation.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1477
Author(s):  
Woo-Suk Jung ◽  
Ill-Min Chung ◽  
Myeong Ha Hwang ◽  
Seung-Hyun Kim ◽  
Chang Yeon Yu ◽  
...  
Keyword(s):  
Plant Growth ◽  
In Vitro Culture ◽  
Light Emitting Diodes ◽  
Photosynthetic Pigment ◽  
Antioxidant Properties ◽  
Light Sources ◽  
Light Emitting ◽  
Cell Defense ◽  
Led Irradiation

Light is a key factor that affects phytochemical synthesis and accumulation in plants. Due to limitations of the environment or cultivated land, there is an urgent need to develop indoor cultivation systems to obtain higher yields with increased phytochemical concentrations using convenient light sources. Light-emitting diodes (LEDs) have several advantages, including consumption of lesser power, longer half-life, higher efficacy, and wider variation in the spectral wavelength than traditional light sources; therefore, these devices are preferred for in vitro culture and indoor plant growth. Moreover, LED irradiation of seedlings enhances plant biomass, nutrient and secondary metabolite levels, and antioxidant properties. Specifically, red and blue LED irradiation exerts strong effects on photosynthesis, stomatal functioning, phototropism, photomorphogenesis, and photosynthetic pigment levels. Additionally, ex vitro plantlet development and acclimatization can be enhanced by regulating the spectral properties of LEDs. Applying an appropriate LED spectral wavelength significantly increases antioxidant enzyme activity in plants, thereby enhancing the cell defense system and providing protection from oxidative damage. Since different plant species respond differently to lighting in the cultivation environment, it is necessary to evaluate specific wavebands before large-scale LED application for controlled in vitro plant growth. This review focuses on the most recent advances and applications of LEDs for in vitro culture organogenesis. The mechanisms underlying the production of different phytochemicals, including phenolics, flavonoids, carotenoids, anthocyanins, and antioxidant enzymes, have also been discussed.

In vitro flowering and fruiting in Portulaca pilosa L.

2021 ◽  
Vol 140 ◽  
pp. 1-3
Author(s):  
Yuping Xiong ◽  
Shuangyan Chen ◽  
Zhenpeng Wei ◽  
Xincheng Yu ◽  
Jinhui Pang ◽  
...  
Keyword(s):  
In Vitro Flowering

scholarly journals TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability

2021 ◽  
Vol 12 (5) ◽  
Author(s):  
Sha Zhou ◽  
Jianhong Peng ◽  
Liuniu Xiao ◽  
Caixia Zhou ◽  
Yujing Fang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Disease Free Survival ◽  
Free Survival ◽  
Epigenetic Regulator ◽  
Crc Management ◽  
Disease Free ◽  
Resistance To Chemotherapy

AbstractResistance to chemotherapy remains the major cause of treatment failure in patients with colorectal cancer (CRC). Here, we identified TRIM25 as an epigenetic regulator of oxaliplatin (OXA) resistance in CRC. The level of TRIM25 in OXA-resistant patients who experienced recurrence during the follow-up period was significantly higher than in those who had no recurrence. Patients with high expression of TRIM25 had a significantly higher recurrence rate and worse disease-free survival than those with low TRIM25 expression. Downregulation of TRIM25 dramatically inhibited, while overexpression of TRIM25 increased, CRC cell survival after OXA treatment. In addition, TRIM25 promoted the stem cell properties of CRC cells both in vitro and in vivo. Importantly, we demonstrated that TRIM25 inhibited the binding of E3 ubiquitin ligase TRAF6 to EZH2, thus stabilizing and upregulating EZH2, and promoting OXA resistance. Our study contributes to a better understanding of OXA resistance and indicates that inhibitors against TRIM25 might be an excellent strategy for CRC management in clinical practice.

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