Sonication-assisted Agrobacterium -mediated transformation of soybean [ Glycine max (L.) Merrill] embryogenic suspension culture tissue

1998 ◽  
Vol 17 (6-7) ◽  
pp. 482-488 ◽  
Author(s):  
H. N. Trick ◽  
J. J. Finer
Keyword(s):  
Glycine Max ◽  
Suspension Culture ◽  
Agrobacterium Mediated Transformation ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture ◽  
Glycine Max L

Cryopreservation of an embryogenic suspension culture of Picea sitchensis and subsequent plant regeneration

1993 ◽  
Vol 8 (1-4) ◽  
pp. 156-162 ◽  
Author(s):  
Jens I. Find ◽  
Franz Floto ◽  
Peter Krogstrup ◽  
Jette Dahl M⊘ller ◽  
Jens V. N⊘rgaard ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Picea Sitchensis ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

Refined glufosinate selection in Agrobacterium -mediated transformation of soybean [ Glycine max (L.) Merrill]

2004 ◽  
Vol 22 (7) ◽  
pp. 478-482 ◽  
Author(s):  
P. Zeng ◽  
D. A. Vadnais ◽  
Z. Zhang ◽  
J. C. Polacco
Keyword(s):  
Glycine Max ◽  
Agrobacterium Mediated Transformation ◽  
Glycine Max L

Fertile wheat (Triticum aestivum L.) regenerants from protoplasts of embryogenic suspension culture

1994 ◽  
Vol 38 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J�nos Pauk ◽  
Zolt�n Kert�sz ◽  
Barnab�s Jenes ◽  
L�szl� Purnhauser ◽  
Outi Manninen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Suspension Culture ◽  
Triticum Aestivum L ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

scholarly journals Regeneration of soybean via embryogenic suspension culture

2001 ◽  
Vol 58 (4) ◽  
pp. 753-758 ◽  
Author(s):  
Annette Droste ◽  
Paulo César Pimentel Leite ◽  
Giancarlo Pasquali ◽  
Elsa Cristina de Mundstock ◽  
Maria Helena Bodanese-Zanettini
Keyword(s):  
Somatic Embryos ◽  
Induction Medium ◽  
Regeneration System ◽  
Breeding Programs ◽  
Immature Cotyledon ◽  
Maturation Medium ◽  
Embryogenic Suspension ◽  
Immature Seeds ◽  
Embryogenic Suspension Culture ◽  
Glycine Max L

In an attempt to establish an alternative plant regeneration system for soybean [Glycine max (L.) Merrill] cultivars used in Brazilian breeding programs, ten genotypes were tested for their embryogenic potential. Cotyledons were removed as explants from immature seeds harvested from field-grown plants. After 45 days on induction medium, the number of responding cotyledons and the number of somatic embryos per immature cotyledon were evaluated. The percentage of explants that produced somatic embryos varied from 1 to 70% among cultivars. The average number of somatic embryos produced per cotyledon pair ranged from 0.01 to 10.3 with a mean of 3.4. Suspension cultures were initiated with three Agrobacterium tumefaciens susceptible cultivars. Suspensions were successfully developed from Bragg and IAS5 cultivars. The packed cell volume, in one-month growth, increased 8.1 fold for Bragg and 3.5 fold for IAS5 and the fresh weight increased 6.6 and 2.8 fold, respectively. The cultivars differed for the analysed parameters. All tissue from each cultivar was transferred to the maturation medium and subsequently to the germination medium. The germination frequency was 45.7 and 54.9% for Bragg and IAS5, respectively. Plants were gradually exposed to ambient humidity over one week and then planted in soil. All plants yielded seeds in the greenhouse.

Induction of Callose Formation by Manganese in Cell Suspension Culture and Leaves of Soybean (Glycine max L.)

1993 ◽  
Vol 142 (1) ◽  
pp. 67-73 ◽  
Author(s):  
A.H. Wissemeier ◽  
A. Hergenröder ◽  
G. Mix-Wagner ◽  
W.J. Horst
Keyword(s):  
Glycine Max ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Glycine Max L ◽  
Callose Formation
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