scholarly journals Regeneration of soybean via embryogenic suspension culture

2001 ◽  
Vol 58 (4) ◽  
pp. 753-758 ◽  
Author(s):  
Annette Droste ◽  
Paulo César Pimentel Leite ◽  
Giancarlo Pasquali ◽  
Elsa Cristina de Mundstock ◽  
Maria Helena Bodanese-Zanettini
Keyword(s):  
Somatic Embryos ◽  
Induction Medium ◽  
Regeneration System ◽  
Breeding Programs ◽  
Immature Cotyledon ◽  
Maturation Medium ◽  
Embryogenic Suspension ◽  
Immature Seeds ◽  
Embryogenic Suspension Culture ◽  
Glycine Max L

In an attempt to establish an alternative plant regeneration system for soybean [Glycine max (L.) Merrill] cultivars used in Brazilian breeding programs, ten genotypes were tested for their embryogenic potential. Cotyledons were removed as explants from immature seeds harvested from field-grown plants. After 45 days on induction medium, the number of responding cotyledons and the number of somatic embryos per immature cotyledon were evaluated. The percentage of explants that produced somatic embryos varied from 1 to 70% among cultivars. The average number of somatic embryos produced per cotyledon pair ranged from 0.01 to 10.3 with a mean of 3.4. Suspension cultures were initiated with three Agrobacterium tumefaciens susceptible cultivars. Suspensions were successfully developed from Bragg and IAS5 cultivars. The packed cell volume, in one-month growth, increased 8.1 fold for Bragg and 3.5 fold for IAS5 and the fresh weight increased 6.6 and 2.8 fold, respectively. The cultivars differed for the analysed parameters. All tissue from each cultivar was transferred to the maturation medium and subsequently to the germination medium. The germination frequency was 45.7 and 54.9% for Bragg and IAS5, respectively. Plants were gradually exposed to ambient humidity over one week and then planted in soil. All plants yielded seeds in the greenhouse.

scholarly journals EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN

2007 ◽  
Vol 13 (1) ◽  
pp. 33-38
Author(s):  
Edy Setiti Wida Utami ◽  
Issirep Soemardi ◽  
Taryono Taryono ◽  
Endang Semiarti
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Leaf Explants ◽  
Gellan Gum ◽  
Development Pattern ◽  
Induction Medium ◽  
Embryo Germination ◽  
Maturation Medium ◽  
One Year

Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i. NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.

scholarly journals Regeneration of Bigleaf Magnolia by Somatic Embryogenesis

HortScience ◽  
1993 ◽  
Vol 28 (6) ◽  
pp. 672-673 ◽  
Author(s):  
S.A. Merkle ◽  
B.A. Watson-Pauley
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Casein Hydrolysate ◽  
Basal Medium ◽  
Induction Medium ◽  
Immature Seeds ◽  
Dichlorophenoxy Acetic Acid ◽  
Adventive Embryos

Bigleaf magnolia (Magnolia macrophylla Michx.) cultures were initiated from immature seeds on an induction medium containing 9.0 μm 2,4-D, 1.1μm BA, and 1 g casein hydrolysate/liter. After 2 months on induction medium, one culture produced adventive embryos. Clumps of embryos transferred to liquid induction medium proliferated as nodules, which grew in diameter, but failed to produce embryos while maintained in induction medium. Nodules transferred to basal medium produced clumps of somatic embryos, which continued to produce repetitive embryos with monthly transfer to fresh basal medium. Individual embryos transferred to basal medium lacking casein hydrolysate germinated and leaves expanded. Plantlets derived from these embryos were transferred to potting mix and acclimatized to greenhouse conditions. Chemical names used: (2,4-dichlorophenoxy)acetic acid (2,4-D); N -(phenylmethyl)-lH-purin-6-amine (BA).

The origin and development of somatic embryos following cryopreservation of an embryogenic suspension culture ofPicea sitchensis

PROTOPLASMA ◽  
1994 ◽  
Vol 182 (1-2) ◽  
pp. 65-70 ◽  
Author(s):  
M. M. H. Kristensen ◽  
J. I. Find ◽  
F. Floto ◽  
J. D. M�ller ◽  
J. V. N�rgaard ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Somatic Embryos ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

Cryopreservation of an embryogenic suspension culture of Picea sitchensis and subsequent plant regeneration

1993 ◽  
Vol 8 (1-4) ◽  
pp. 156-162 ◽  
Author(s):  
Jens I. Find ◽  
Franz Floto ◽  
Peter Krogstrup ◽  
Jette Dahl M⊘ller ◽  
Jens V. N⊘rgaard ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Picea Sitchensis ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

EFFECT OF 2,4-D AND NAA ON SOMATIC EMBRYOGENESIS FROM APICAL PORTION OF GROUNDNUT (ARACHIS HYPOGAEA L.)

2021 ◽  
Vol 1 (7) ◽  
pp. 119-124
Author(s):  
Muniappan V ◽  
Manivel P ◽  
Prabakaran V ◽  
Palanivel S ◽  
Parvathi S
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Arachis Hypogaea ◽  
Somatic Embryos ◽  
Mature Embryo ◽  
Induction Medium ◽  
Embryogenic Cultures ◽  
Apical Portion ◽  
Arachis Hypogaea L ◽  
Somatic Embryo Induction

Somatic embryogenesis was carried out epicotyl portion of the mature embryo/apical portion. The somatic embryo induction medium containing 2,4-D or NAA (10.0 to 50.0 mg/l). Of the two concentrations tested 2,4-D (30.0mg/l) recorded the highest percentage of response followed by NAA (30.0mg/l). But the highest number of somatic embryo were recorded in 30.0mg/l of 2,4-D followed by NAA. The apical portion of the mature embryo formed direct embryos without any intervention of callus. The maximum percentage of embryogenic cultures were noticed in 30.0mg/l of 2,4-D followed by NAA at 30.0mg/l. for the differentiation of somatic embryos, the embryogenic masses were transferred to medium without any growth regulator. The maximum number of somatic embryos per culture was recorded in 30 mg/l of 2,4-D followed by 30.0 mg/l of NAA. Keywords: Arachis hypogaea L.,Somatic Embryogenesis, 2,4-D and NAA

scholarly journals The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets

2021 ◽  
Vol 12 ◽  
Author(s):  
Antonia Maiara Marques do Nascimento ◽  
Luiza Giacomolli Polesi ◽  
Franklin Panato Back ◽  
Neusa Steiner ◽  
Miguel Pedro Guerra ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Amino Acid ◽  
Somatic Embryos ◽  
Pinus Halepensis ◽  
Morphological Characteristics ◽  
Chemical Environment ◽  
Acid Mixture ◽  
Maturation Stage ◽  
Maturation Medium ◽  
Pinus Spp

Changes in the chemical environment at the maturation stage in Pinus spp. somatic embryogenesis will be a determinant factor in the conversion of somatic embryos to plantlets. Furthermore, the study of biochemical and morphological aspects of the somatic embryos could enable the improvement of somatic embryogenesis in Pinus spp. In the present work, the influence of different amino acid combinations, carbohydrate sources, and concentrations at the maturation stage of Pinus radiata D. Don and Pinus halepensis Mill. was analyzed. In P. radiata, the maturation medium supplemented with 175 mM of sucrose and an increase in the amino acid mixture (1,100 mgL–1 of L-glutamine, 1,050 mgL–1 of L-asparagine, 350 mgL–1 of L-arginine, and 35 mgL–1 of L-proline) promoted bigger embryos, with a larger stem diameter and an increase in the number of roots in the germinated somatic embryos, improving the acclimatization success of this species. In P. halepensis, the maturation medium supplemented with 175 mM of maltose improved the germination of somatic embryos. The increase in the amount of amino acids in the maturation medium increased the levels of putrescine in the germinated somatic embryos of P. halepensis. We detected significant differences in the amounts of polyamines between somatic plantlets of P. radiata and P. halepensis; putrescine was less abundant in both species. For the first time, in P. radiata and P. halepensis somatic embryogenesis, we detected the presence of cadaverine, and its concentration changed according to the species.

scholarly journals Genetic Diversity Studies in MAGIC Population of Soybean (Glycine max (L.) Merrill) Based on Mahalanobis D2 Distance

2021 ◽  
pp. 18-25
Author(s):  
Bidush Ranjan Swar ◽  
V. Swarnalatha ◽  
M. Rajendar Reddy ◽  
S. Vanisree
Keyword(s):  
Genetic Diversity ◽  
Seed Weight ◽  
Quantitative Traits ◽  
Genetic Recombination ◽  
Breeding Programs ◽  
Total Genetic Diversity ◽  
Glycine Max L ◽  
Diversity Studies ◽  
100 Seed Weight ◽  
Number Of Branches

Soybean MAGIC lines are highly variable breeding material which utilizes both recent and historic genetic recombination events. Present investigation was carried out to assess the genetic diversity present among 95 soybean MAGIC lines along with six checks for 10 different quantitative traits. All the genotypes were grouped into 16 clusters by performing Tocher’s clustering method using Mahalanobis D2 distance. Cluster I was the largest comprising of 30 genotypes followed by cluster II (23 genotypes), cluster X (15 genotypes) and cluster IX (9 genotypes). The maximum genetic distance (D2) was observed between cluster XI and XV (168.37) followed by cluster III and XV (164.3), cluster X and XV (149.64) as well as between cluster XII and cluster XVI (145.99). The cluster mean for most of the traits were high in cluster I and cluster XVI. Oil content contributed maximum (23.86%) towards total genetic diversity followed by number of pods plant-1 (18.97%), seed yield plant-1 (18.63%), 100 seed weight (11.05%) and number of branches plant-1 (10.16%) traits. The soybean MAGIC lines belong to the cluster XI (6-120) and cluster XV (6-30, 6-31, 6-5) were found to be the most divergent hence can be utilised in the recombination breeding programs to exploit maximum heterosis.

scholarly journals Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes

2012 ◽  
Vol 38 (No. 2) ◽  
pp. 69-74 ◽  
Author(s):  
M. Baránek ◽  
M. Kadlec ◽  
J. Raddová ◽  
M. Vachůn ◽  
M. Pidra
Keyword(s):  
Genetic Diversity ◽  
Glycine Max ◽  
Random Amplified Polymorphic Dna ◽  
Breeding Programs ◽  
Low Level ◽  
Low Degree ◽  
Soybean Genotypes ◽  
Glycine Max L ◽  
Rapd Polymorphism ◽  
Selection Of

The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process. 

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