cDNA cloning and chromosomal mapping of mouse fast skeletal muscle troponin T

1997 ◽  
Vol 8 (5) ◽  
pp. 346-348 ◽  
Author(s):  
Anne Koch ◽  
Todd S. -C. Juan ◽  
Nancy A. Jenkins ◽  
Debra J. Gilbert ◽  
Neal G. Copeland ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cdna Cloning ◽  
Troponin T ◽  
Chromosomal Mapping ◽  
Fast Skeletal Muscle

scholarly journals Conformational modulation of slow skeletal muscle troponin T by an NH2-terminal metal-binding extension

2000 ◽  
Vol 279 (4) ◽  
pp. C1067-C1077 ◽  
Author(s):  
Jian-Ping Jin ◽  
Aihua Chen ◽  
Ozgur Ogut ◽  
Qi-Quan Huang
Keyword(s):  
Skeletal Muscle ◽  
Monoclonal Antibody ◽  
Metal Binding ◽  
Metal Ion ◽  
Troponin I ◽  
Troponin T ◽  
Terminal Region ◽  
Sequence Motif ◽  
Fast Skeletal Muscle ◽  
Slow Skeletal Muscle

Troponin T (TnT) is an essential element in the thin filament Ca2+-regulatory system controlling striated muscle contraction. Alternative RNA splicing generates developmental and muscle type-specific TnT isoforms differing in the hypervariable NH2-terminal region. Using avian fast skeletal muscle TnT containing a metal-binding segment, we have demonstrated a role of the NH2-terminal domain in modulating the conformation of TnT (Wang J and Jin JP. Biochemistry 37: 14519–14528, 1998). To further investigate the structure-function relationship of TnT, the present study constructed and characterized a recombinant protein in which the metal-binding peptide present in avian fast skeletal muscle TnT was fused to the NH2 terminus of mouse slow skeletal muscle TnT. Metal ion or monoclonal antibody binding to the NH2-terminal extension induced conformational changes in other domains of the model TnT molecule. This was shown by the altered affinity to a monoclonal antibody against the COOH-terminal region and a polyclonal antiserum recognizing multiple epitopes. Protein binding assays showed that metal binding to the NH2-terminal extension had effects on the interaction of TnT with troponin I, troponin C, and most significantly, tropomyosin. The data indicate that the NH2-terminal Tx [4–7 repeats of a sequence motif His-(Glu/Ala)-Glu-Ala-His] extension confers a specific conformational modulation in the slow skeletal muscle TnT.

cDNA cloning and thermodynamic properties of tropomyosin from walleye pollack Theragra chalcogramma fast skeletal muscle

2003 ◽  
Vol 69 (5) ◽  
pp. 1033-1041 ◽  
Author(s):  
Yoshihiro OCHIAI ◽  
Ming-chih HUANG ◽  
Hideto FUKUSHIMA ◽  
Shugo WATABE
Keyword(s):  
Skeletal Muscle ◽  
Thermodynamic Properties ◽  
Cdna Cloning ◽  
Theragra Chalcogramma ◽  
Fast Skeletal Muscle ◽  
Walleye Pollack

cDNA cloning of myosin heavy chain from white croaker fast skeletal muscle and characterization of its complete primary structure

2000 ◽  
Vol 66 (6) ◽  
pp. 1163-1171 ◽  
Author(s):  
Sung Ho Yoon ◽  
Makoto Kakinuma ◽  
Yasushi Hirayama ◽  
Tsuneji Yamamoto ◽  
Shugo Watabe
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Cdna Cloning ◽  
Heavy Chain ◽  
Primary Structure ◽  
Fast Skeletal Muscle ◽  
White Croaker ◽  
Complete Primary Structure

cDNA cloning and deduced amino acid sequence of tropomyosin from fast skeletal muscle of white croaker Pennahia argentata

2001 ◽  
Vol 67 (3) ◽  
pp. 556-558 ◽  
Author(s):  
Yoshihiro Ochiai ◽  
Kashfia Ahmed ◽  
Md Nazmul Ahsan ◽  
Daisuke Funabara ◽  
Misako Nakaya ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cdna Cloning ◽  
Fast Skeletal Muscle ◽  
White Croaker ◽  
Pennahia Argentata

The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T

1987 ◽  
Vol 8 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Margaret M. Briggs ◽  
Jim J. -C. Lin ◽  
Frederick H. Schachat
Keyword(s):  
Skeletal Muscle ◽  
Troponin T ◽  
Fast Skeletal Muscle ◽  
Amino Terminal
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