Establishment of a protocol for large-scale gene expression analyses of laser capture microdissected bladder tissue

2012 ◽  
Vol 30 (6) ◽  
pp. 853-859
Author(s):  
M. Horstmann ◽  
B. Foerster ◽  
N. Brader ◽  
H. John ◽  
C. Maake
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Bladder Tissue ◽  
Gene Expression Analyses ◽  
Laser Capture ◽  
Laser Capture Microdissected

scholarly journals Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs

PLoS Biology ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. e3001214
Author(s):  
Noam Cohen ◽  
Hassan Massalha ◽  
Shani Ben-Moshe ◽  
Adi Egozi ◽  
Milena Rozenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Single Molecule ◽  
Mesenchymal Cell ◽  
Lymphoid Follicle ◽  
Lymphoid Follicles ◽  
Mouse Small Intestine ◽  
Laser Capture ◽  
Laser Capture Microdissected

The intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related to the scarcity and small sizes of ILFs and their follicle-associated epithelium (FAE) impeded the characterization of their spatial gene expression programs. Here, we combined RNA sequencing of laser capture microdissected tissues with single-molecule transcript imaging to obtain a spatial gene expression map of the ILF and its associated FAE in the mouse small intestine. We identified zonated expression programs in both follicles and FAEs, with a decrease in enterocyte antimicrobial and absorption programs and a partial induction of expression programs normally observed at the villus tip. We further identified Lepr+ subepithelial telocytes at the FAE top, which are distinct from villus tip Lgr5+ telocytes. Our analysis exposes the epithelial and mesenchymal cell states associated with ILFs.

ADAM12 and ADAM17 Gene Expression in Laser-capture Microdissected and Non-microdissected Breast Tumors

2011 ◽  
Vol 17 (2) ◽  
pp. 375-385 ◽  
Author(s):  
Diana Narita ◽  
Edward Seclaman ◽  
Razvan Ilina ◽  
Natalia Cireap ◽  
Sorin Ursoniu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Breast Tumors ◽  
Laser Capture ◽  
Laser Capture Microdissected

scholarly journals Gene expression analysis of interferon κ in laser capture microdissected cervical epithelium

2008 ◽  
Vol 381 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Correne A. DeCarlo ◽  
Nicholas G. Escott ◽  
Julieta Werner ◽  
Kerry Robinson ◽  
Paul F. Lambert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cervical Epithelium ◽  
Laser Capture ◽  
Laser Capture Microdissected

scholarly journals Smoking-Related Gene Expression in Laser Capture-Microdissected Human Lung

2009 ◽  
Vol 15 (24) ◽  
pp. 7562-7570 ◽  
Author(s):  
X.-L. Tan ◽  
T. Wang ◽  
S. Xiong ◽  
S. V. Kumar ◽  
W. Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Related Gene ◽  
Laser Capture ◽  
Laser Capture Microdissected
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