scholarly journals Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs

PLoS Biology ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. e3001214
Author(s):  
Noam Cohen ◽  
Hassan Massalha ◽  
Shani Ben-Moshe ◽  
Adi Egozi ◽  
Milena Rozenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Single Molecule ◽  
Mesenchymal Cell ◽  
Lymphoid Follicle ◽  
Lymphoid Follicles ◽  
Mouse Small Intestine ◽  
Laser Capture ◽  
Laser Capture Microdissected

The intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related to the scarcity and small sizes of ILFs and their follicle-associated epithelium (FAE) impeded the characterization of their spatial gene expression programs. Here, we combined RNA sequencing of laser capture microdissected tissues with single-molecule transcript imaging to obtain a spatial gene expression map of the ILF and its associated FAE in the mouse small intestine. We identified zonated expression programs in both follicles and FAEs, with a decrease in enterocyte antimicrobial and absorption programs and a partial induction of expression programs normally observed at the villus tip. We further identified Lepr+ subepithelial telocytes at the FAE top, which are distinct from villus tip Lgr5+ telocytes. Our analysis exposes the epithelial and mesenchymal cell states associated with ILFs.

scholarly journals Spatial expression programs of the intestinal follicle-associated epithelium

2021 ◽  
Author(s):  
Noam Cohen ◽  
Hassan Massalha ◽  
Shani Ben-Moshe ◽  
Adi Egozi ◽  
Milena Rozenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Single Molecule ◽  
Mesenchymal Cell ◽  
Lymphoid Follicles ◽  
Mouse Small Intestine ◽  
Follicle Associated Epithelium ◽  
Laser Capture ◽  
Laser Capture Microdissected

AbstractThe intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related to the scarcity and small sizes of ILFs and their follicle-associated epithelium (FAE) impeded the characterization of their spatial gene expression programs. Here, we combined RNA sequencing of laser capture microdissected tissues with single molecule transcript imaging to obtain a spatial gene expression map of the ILF and its associated FAE in the mouse small intestine. We identified zonated expression programs in both follicles and FAEs, with a decrease in enterocyte anti-microbial and absorption programs and a partial induction of expression programs normally observed at the villus tip. We further identified Lepr+ sub-epithelial telocytes at the FAE top, which are distinct from villus-tip Lgr5+ telocytes. Our analysis exposes the epithelial and mesenchymal cell states associated with ILFs.

scholarly journals Characterization of glomerular diseases using proteomic analysis of laser capture microdissected glomeruli

2012 ◽  
Vol 25 (5) ◽  
pp. 709-721 ◽  
Author(s):  
Anjali A Satoskar ◽  
John P Shapiro ◽  
Cherri N Bott ◽  
Huijuan Song ◽  
Gyongyi M Nadasdy ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Glomerular Diseases ◽  
Laser Capture ◽  
Laser Capture Microdissected

ADAM12 and ADAM17 Gene Expression in Laser-capture Microdissected and Non-microdissected Breast Tumors

2011 ◽  
Vol 17 (2) ◽  
pp. 375-385 ◽  
Author(s):  
Diana Narita ◽  
Edward Seclaman ◽  
Razvan Ilina ◽  
Natalia Cireap ◽  
Sorin Ursoniu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Breast Tumors ◽  
Laser Capture ◽  
Laser Capture Microdissected

scholarly journals P03.03 Organization, function and gene expression of tertiary lymphoid structures in PDAC resembles lymphoid follicles in secondary lymphoid organs

2020 ◽  
Vol 8 (Suppl 2) ◽  
pp. A23.1-A23
Author(s):  
M Thelen ◽  
MA García-Márquez ◽  
T Nestler ◽  
S Wagener-Ryczek ◽  
J Lehmann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Expression Patterns ◽  
Lymphoid Organs ◽  
Immunofluorescence Staining ◽  
Ductal Adenocarcinoma ◽  
Lymphoid Follicles ◽  
Tertiary Lymphoid Structures ◽  
Lymphoid Structures ◽  
Secondary Lymphoid Organs

BackgroundSecondary lymphoid organs (SLO) are involved in induction and enhancement of anti-tumor immune responses on different tumor entities. Recent evidence suggests that anti-tumor immune responses may also be induced or enhanced in the tumor microenvironment in so called tertiary lymphoid structures (TLS). It is assumed that TLS represent a hotspot for T cell priming, B cell activation, and differentiation, leading to cellular and humoral anti-tumor immune response.MethodsFFPE-slides of 120 primary pancreatic ductal adenocarcinoma (PDAC) patients were immunohistochemically (IHC) stained for CD20, CD3, CD8 and HLA-ABC to analyze spatial distribution of tumor-infiltrating lymphocytes. 5-color immunofluorescence staining was performed to further investigate structural components of TLS in comparison to lymphoid follicles in SLOs. Microscope-based laser microdissection and Nanostring-base RNA expression analysis were used to compare gene expression in PDAC, TLS, SLOs and normal pancreatic tissue.ResultsTLS were frequently detected in PDAC and were mainly localized along the invasive tumor margin. In less than 10% of the cases TLS were infiltrating the tumors. Interestingly, 20% of the patients had no TLS. Results of TLS will be correlated with clinical parameters, Immunoscore and immune escape mechanisms. 5-color Immunofluorescence staining revealed similar organization and function of TLS and SLO. Finally, gene expression analyzed by Nanostring revealed largely overlapping expression patterns in TLS and SLO.ConclusionsThe results clearly demonstrate close similarities between SLO and TLS in terms of composition, distribution and gene expression Patterns.Disclosure InformationM. Thelen: None. M.A. García-Márquez: None. T. Nestler: None. S. Wagener-Ryczek: None. J. Lehmann: None. E. Staib: None. F. Popp: None. F. Gebauer: None. P. Lohneis: None. M. Odenthal: None. S. Merkelbach-Bruse: None. C. Bruns: None. K. Wennhold: None. M. von Bergwelt-Baildon: None. H.A. Schlößer: None.

scholarly journals Gene expression analysis of interferon κ in laser capture microdissected cervical epithelium

2008 ◽  
Vol 381 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Correne A. DeCarlo ◽  
Nicholas G. Escott ◽  
Julieta Werner ◽  
Kerry Robinson ◽  
Paul F. Lambert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cervical Epithelium ◽  
Laser Capture ◽  
Laser Capture Microdissected
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