Adenovirus-mediated stem cell leukemia gene transfer induces rescue of interstitial cells of Cajal in ICC-loss mice

2010 ◽  
Vol 25 (5) ◽  
pp. 557-566 ◽  
Author(s):  
Fan Li ◽  
Lin Zhang ◽  
Chunxue Li ◽  
Bing Ni ◽  
Yuzhang Wu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Gene Transfer ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cell Leukemia ◽  
Cells Of Cajal ◽  
Stem Cell Leukemia

scholarly journals Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal

2020 ◽  
Vol 15 (1) ◽  
pp. 83-91
Author(s):  
Chen Li ◽  
Biao Qian ◽  
Zhao Ni ◽  
Qinzhang Wang ◽  
Zixiong Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Interstitial Cells Of Cajal ◽  
Lentiviral Vector ◽  
Transfection Efficiency ◽  
Interstitial Cells ◽  
Human Stem Cell ◽  
Cell Leukemia ◽  
Cells Of Cajal ◽  
Stem Cell Leukemia

AbstractThis study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders. In this study, the human SCL gene was successfully cloned, and the recombinant lentivirus GV287-SCL was successfully constructed. The titer of the recombinant lentivirus was 5 × 108 TU /mL. After transfecting the ICCs with the lentiviral vector at different MOIs, the optimal MOI was determined to be 10.0, and the optimal transfection time was determined to be 3 days. The amplification product of the lentivirus transfection group was consistent with the target fragment, indicating that the SCL gene had been successfully introduced into ICCs. In conclusion, the recombinant lentiviral vector GV287-SCL was successfully constructed and transfected into the in vitro cultured ICCs. The successful expression of SCL in ICCs may provide an experimental basis for the in vivo transfection of the SCL gene.

Roles of Stem Cell Factor on Loss of Interstitial Cells of Cajal in Bladder of Diabetic Rats

Urology ◽  
2011 ◽  
Vol 78 (6) ◽  
pp. 1443.e1-1443.e6 ◽  
Author(s):  
Wei Chen ◽  
Chonghe Jiang ◽  
Xiyu Jin ◽  
Wenhao Shen ◽  
Bo Song ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Rats ◽  
Cells Of Cajal

T1364 The Effect of Stem Cell Factor On Interstitial Cells of Cajal in the Colon of Diabetic Mice

2008 ◽  
Vol 134 (4) ◽  
pp. A-539-A-540
Author(s):  
Lin Lin ◽  
Liming Xu ◽  
Yurong Tang ◽  
Hongjie Zhang ◽  
Li Xueliang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Mice ◽  
Cells Of Cajal

scholarly journals Effect of stem cell factor on colon interstitial cells of Cajal in murine with diabetes mellitus

2008 ◽  
Vol 16 (12) ◽  
pp. 1294
Author(s):  
Li-Ming Xu ◽  
Lin Lin ◽  
Yu-Rong Tang ◽  
Hong-Jie Zhang ◽  
Xue-Liang Li
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal

Effect of stem cell factor on colon interstitial cells of Cajal in murine with diabetes mellitus

2008 ◽  
Vol 16 (12) ◽  
pp. 1294
Author(s):  
Li-Ming Xu ◽  
Lin Lin ◽  
Yu-Rong Tang ◽  
Hong-Jie Zhang ◽  
Xue-Liang Li
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal

Dose-dependent and time-limited proliferation of cultured murine interstitial cells of Cajal in response to stem cell factor

Life Sciences ◽  
2002 ◽  
Vol 70 (20) ◽  
pp. 2367-2376 ◽  
Author(s):  
Masanori Nakahara ◽  
Koji Isozaki ◽  
Jean-Marie Vanderwinden ◽  
Yasuhisa Shinomura ◽  
Yukihiko Kitamura ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal ◽  
Dose Dependent
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