T-oligos as differential modulators of human scalp hair growth and pigmentation: a new “time lapse system” for studying human skin and hair follicle biology in vitro?

2008 ◽  
Vol 300 (4) ◽  
pp. 155-159 ◽  
Author(s):  
Ina Hadshiew ◽  
Kirsten Barre ◽  
Enikö Bódó ◽  
Wolfgang Funk ◽  
Ralf Paus
Keyword(s):  
Hair Follicle ◽  
Human Skin ◽  
Hair Growth ◽  
Scalp Hair ◽  
Time Lapse ◽  
Human Scalp Hair ◽  
New Time

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

scholarly journals Human Scalp Hair Follicles Are Both a Target and a Source of Prolactin, which Serves as an Autocrine and/or Paracrine Promoter of Apoptosis-Driven Hair Follicle Regression

2006 ◽  
Vol 168 (3) ◽  
pp. 748-756 ◽  
Author(s):  
Kerstin Foitzik ◽  
Karoline Krause ◽  
Franziska Conrad ◽  
Motonobu Nakamura ◽  
Wolfang Funk ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Scalp Hair ◽  
Hair Follicles ◽  
Human Scalp Hair

scholarly journals Estrogens and Human Scalp Hair Growth—Still More Questions than Answers

2004 ◽  
Vol 122 (3) ◽  
pp. 840-842 ◽  
Author(s):  
Franziska Conrad ◽  
Ulrich Ohnemus ◽  
Enikö Bodo ◽  
Albrecht Bettermann ◽  
Ralf Paus
Keyword(s):  
Hair Growth ◽  
Scalp Hair ◽  
Human Scalp Hair

scholarly journals Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture

2019 ◽  
Vol 1 (3) ◽  
pp. 12-19
Author(s):  
Mahendra Kumar Trivedi ◽  
Snehasis Jana
Keyword(s):  
Organ Culture ◽  
Hair Follicle ◽  
Test Item ◽  
Hair Growth ◽  
Sexual Communication ◽  
Growth Promotion ◽  
Growth Promoter ◽  
Energy Healing ◽  
Hair Follicle Organ Culture

Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc.

Limitations of human occipital scalp hair follicle organ culture for studying the effects of minoxidil as a hair growth enhancer

2004 ◽  
Vol 13 (10) ◽  
pp. 635-642 ◽  
Author(s):  
Markus Magerl ◽  
Ralf Paus ◽  
Nilofer Farjo ◽  
Sven Muller-Rover ◽  
Eva M. J. Peters ◽  
...  
Keyword(s):  
Organ Culture ◽  
Hair Follicle ◽  
Hair Growth ◽  
Scalp Hair ◽  
Hair Follicle Organ Culture

THE METABOLISM OF TESTOSTERONE BY HUMAN SKIN IN DISORDERS OF HAIR GROWTH

1973 ◽  
Vol 59 (2) ◽  
pp. 345-351 ◽  
Author(s):  
J. S. JENKINS ◽  
S. ASH
Keyword(s):  
Human Skin ◽  
Metabolic Activity ◽  
Hair Growth ◽  
Normal Skin ◽  
Rare Condition ◽  
Testicular Feminization ◽  
Body Hair ◽  
Testosterone Levels ◽  
Localized Failure

SUMMARY The metabolism of testosterone by skin from patients with disorders of hair growth in the presence of normal testosterone levels has been studied in vitro and compared with the metabolic activity of normal skin. The main metabolites formed were dihydrotestosterone, androstenedione, androsterone and androstanedione. In four out of six females with hirsutism, the skin produced increased amounts of dihydrotestosterone and also androstenedione. A patient with the rare condition of unilateral hirsutism showed no difference in metabolic activity between the hirsute and non-hirsute sides. In localized failure of androgenically determined hair growth, seen in three beardless males, the metabolism of the hairless area was greater than that of the normally hairy abdomen. Four patients with testicular feminization and complete absence of body hair were studied and the formation of dihydrotestosterone ranged from low to high normal levels.

scholarly journals Review of Hair Follicle Dermal Papilla cells as in vitro screening model for hair growth

2018 ◽  
Vol 40 (5) ◽  
pp. 429-450 ◽  
Author(s):  
Alka Madaan ◽  
Ritu Verma ◽  
Anu T. Singh ◽  
Manu Jaggi
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Dermal Papilla ◽  
In Vitro Screening ◽  
Dermal Papilla Cells ◽  
Screening Model
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