scholarly journals Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture

2019 ◽  
Vol 1 (3) ◽  
pp. 12-19
Author(s):  
Mahendra Kumar Trivedi ◽  
Snehasis Jana
Keyword(s):  
Organ Culture ◽  
Hair Follicle ◽  
Test Item ◽  
Hair Growth ◽  
Sexual Communication ◽  
Growth Promotion ◽  
Growth Promoter ◽  
Energy Healing ◽  
Hair Follicle Organ Culture

Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc.

Limitations of human occipital scalp hair follicle organ culture for studying the effects of minoxidil as a hair growth enhancer

2004 ◽  
Vol 13 (10) ◽  
pp. 635-642 ◽  
Author(s):  
Markus Magerl ◽  
Ralf Paus ◽  
Nilofer Farjo ◽  
Sven Muller-Rover ◽  
Eva M. J. Peters ◽  
...  
Keyword(s):  
Organ Culture ◽  
Hair Follicle ◽  
Hair Growth ◽  
Scalp Hair ◽  
Hair Follicle Organ Culture

scholarly journals In Vitro Assessment of Biofield Energy Healing Treatment on Hair Growth by Enhanced Proliferation of Human Follicular Dermal Papilla Cells (HFDPCs)

2018 ◽  
Vol 4 (1) ◽  
pp. 30-44
Author(s):  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Promoter ◽  
Androgenic Alopecia ◽  
Dermal Papilla Cells ◽  
Test Items ◽  
Energy Healing ◽  
The One

Patterned hair loss or androgenic alopecia is a disorder affecting millions of peoples, in which hair sheds without adequate regrowth. Authors propose a new approach to increase hair growth. In this study, authors examined the in vitro potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test items, Dulbecco’s Modified Eagle Medium (DMEM) on the Human Follicular Dermal Papilla Culture Cells (HFDPC) for the assessment of growth and development. The test items (DMEM) were divided into three parts. First part did not receive any sort of treatment and defined as the untreated DMEM group. The second and third parts were treated with the one-time and two-times Biofield Energy Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and coded as the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively. The Biofield Energy Healing Treatment was provided by a renowned Biofield Energy Healer (The Trivedi Effect®), remotely for ~5 minutes under standard laboratory conditions through unique energy transmission process. The experimental results showed that one-time Biofield Energy Treated DMEM showed 70.24% and two-times Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased the proliferation of dermal papilla cells by 207.62% as compared to the untreated DMEM group. The overall results demonstrated that the Biofield Energy Healing Treatment significantly increased the proliferation of human hair follicle dermal papilla cells in vitro. Therefore, the Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter in vivo against different types of skin injuries, hair disorders, and opens new research venues.

scholarly journals Ficus benghalensis as Potential Inhibitor of 5α-Reductase for Hair Growth Promotion: In Vitro, In Silico, and In Vivo Evaluation

2021 ◽  
Vol 12 ◽  
Author(s):  
Jawaria Iltaf ◽  
Sobia Noreen ◽  
Muhammad Fayyaz ur Rehman ◽  
Shazia Akram Ghumman ◽  
Fozia Batool ◽  
...  
Keyword(s):  
In Silico ◽  
Hair Growth ◽  
De Novo ◽  
Growth Promotion ◽  
Hair Follicles ◽  
Binding Energies ◽  
Ficus Benghalensis ◽  
Rp Hplc

The screening of hair follicles, dermal papilla cells, and keratinocytes through in vitro, in vivo, and histology has previously been reported to combat alopecia. Ficus benghalensis has been used conventionally to cure skin and hair disorders, although its effect on 5α-reductase II is still unknown. Currently, we aim to analyze the phytotherapeutic impact of F. benghalensis leaf extracts (FBLEs) for promoting hair growth in rabbits along with in vitro inhibition of the steroid isozyme 5α-reductase II. The inhibition of 5α-reductase II by FBLEs was assessed by RP-HPLC, using the NADPH cofactor as the reaction initiator and Minoxin (5%) as a positive control. In silico studies were performed using AutoDock Vina to visualize the interaction between 5α-reductase II and the reported phytoconstituents present in FBLEs. Hair growth in female albino rabbits was investigated by applying an oral dose of the FBLE formulation and control drug to the skin once a day. The skin tissues were examined by histology to see hair follicles. Further, FAAS, FTIR, and antioxidants were performed to check the trace elements and secondary metabolites in the FBLEs. The results of RP-HPLC and the binding energies showed that FBLEs reduced the catalytic activity of 5α-reductase II and improved cell proliferation in rabbits. The statistical analysis (p < 0.05 or 0.01) and percentage inhibition (>70%) suggested that hydroalcoholic FBLE has more potential in increasing hair growth by elongating hair follicle’s anagen phase. FAAS, FTIR, and antioxidant experiments revealed sufficient concentrations of Zn, Cu, K, and Fe, together with the presence of polyphenols and scavenging activity in FBLE. Overall, we found that FBLEs are potent in stimulating hair follicle maturation by reducing the 5α-reductase II action, so they may serve as a principal choice in de novo drug designing to treat hair loss.

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

scholarly journals Methods in hair research: how to objectively distinguish between anagen and catagen in human hair follicle organ culture

2010 ◽  
Vol 19 (3) ◽  
pp. 305-312 ◽  
Author(s):  
Jennifer Elisabeth Kloepper ◽  
Koji Sugawara ◽  
Yusur Al-Nuaimi ◽  
Erzsébet Gáspár ◽  
Nina van Beek ◽  
...  
Keyword(s):  
Organ Culture ◽  
Hair Follicle ◽  
Human Hair ◽  
Human Hair Follicle ◽  
Hair Follicle Organ Culture

scholarly journals Biological Control of Leaf Spot and Growth Promotion of Eucalyptus Plants by Trichoderma spp.

2018 ◽  
Vol 10 (9) ◽  
pp. 459 ◽  
Author(s):  
Magno Rodrigues Carvalho Filho ◽  
Irene Martins ◽  
Gustavo Henrique Silva Peixoto ◽  
Paulo Henrique Pereira Costa Muniz ◽  
Daniel Diego Costa Carvalho ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Growth Promotion ◽  
Suppressive Effect ◽  
Growth Promoter ◽  
Trichoderma Spp ◽  
Parboiled Rice ◽  
General Terms ◽  
Detached Leaves ◽  
Trichoderma Isolates

This study aimed to evaluate the potential of twelve Trichoderma isolates on eucalyptus leaf spot control induced by Cylindrocladium scoparium and determine the sporulation capacity of the isolates on two substrates (parboiled rice and millet) for selection of potential biocontrol and growth promoter agents, in eucalypt seedlings. In the in vitro experiments, volatile and non-volatile metabolites tests showed different levels of inhibition of the pathogen mycelial growth. In tests on detached leaves, it was observed suppression of disease symptoms with all Trichoderma strains in leaves inoculated with CEN494 isolate of the pathogen, whereas with isolate CEN517 did not found the same efficiency. Sporulation of Trichoderma was higher using parboiled rice as the substrate, compared to millet. In general terms, it was obtained 3.38 × 109 and 2.84 × 109 conidia/g for the two substrates, respectively. Some of these Trichoderma isolates may be used in development of biofungicides for biocontrol of leaf spot, especially CEN262, due to its suppressive effect on C. scoparium, high sporulation, growth promotion capacity and endophytic colonization in eucalyptus seedlings.

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