Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye ( Secale cereale )

Chromosoma ◽  
1996 ◽  
Vol 105 (2) ◽  
pp. 97-103 ◽  
Author(s):  
Andreas Houben ◽  
Ralf G. Kynast ◽  
Ute Heim ◽  
Hanno Hermann ◽  
R. Neil Jones ◽  
...  
Keyword(s):  
Secale Cereale ◽  
B Chromosome ◽  
Heterochromatic Segment ◽  
Molecular Cytogenetic

Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale)

Chromosoma ◽  
1996 ◽  
Vol 105 (2) ◽  
pp. 97-103 ◽  
Author(s):  
Andreas Houben ◽  
Ralf G. Kynast ◽  
Ute Heim ◽  
Hanno Hermann ◽  
R. Neil Jones ◽  
...  
Keyword(s):  
Secale Cereale ◽  
B Chromosome ◽  
Heterochromatic Segment ◽  
Molecular Cytogenetic

scholarly journals Identification of an A-B Chromosome Translocation in Diploid Rye(Secale cereale L.).

1994 ◽  
Vol 44 (3) ◽  
pp. 279-283
Author(s):  
R. Schlegel ◽  
W. Pohler
Keyword(s):  
Secale Cereale ◽  
B Chromosome ◽  
Chromosome Translocation ◽  
Secale Cereale L

Identification of a family of repeated sequences on the rye B chromosome

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 908-913 ◽  
Author(s):  
Michael J. Sandery ◽  
John W. Forster ◽  
Richard Blunden ◽  
R. Neil Jones
Keyword(s):  
Secale Cereale ◽  
Direct Evidence ◽  
B Chromosome ◽  
Repeat Sequence ◽  
B Chromosomes ◽  
Repeated Sequences ◽  
Significant Heterogeneity ◽  
Origin And Evolution ◽  
The Family

A novel family of highly repeated sequences on the B chromosome of rye (Secale cereale) has been identified. The D1100 family has not been detected on the rye A chromosomes and shows little or no homology to any previously described repeat sequence in rye. In addition, different rye species, and different B chromosomes within the same species, show significant heterogeneity in the arrangement of the D1100 sequences. An EcoRI clone of a member of the family has been obtained. These results provide direct evidence for the organisation and nature of the B-chromosome DNA in rye, and they are discussed in relation to the origin and evolution of rye B chromosomes.Key words: B chromosome, Secale cereale, repeated sequences.

Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 706-711 ◽  
Author(s):  
Richard Blunden ◽  
Timothy J. Wilkes ◽  
John W. Forster ◽  
Mar M. Jimenez ◽  
Michael J. Sandery ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Secale Cereale ◽  
Repeated Sequence ◽  
B Chromosome ◽  
Repeated Sequences ◽  
Genomic Hybridization ◽  
The Family

A second family of highly repeated sequences has been identified on the B chromosome of rye (Secale cereale). The E3900 family was detected as a variant band in EcoRI digests of +B DNA. A clone of the basic repeat of the family was obtained, and the organization of the family was investigated by genomic hybridization. The E3900 family has no apparent homology to the A chromosome sequences of rye or other members of the Gramineae. The family has been localized by in situ hybridization to the end of the long arm of the rye B chromosome. The previously characterized E1100 sequence shows in situ hybridization to the same location as the E3900 family. These results are discussed in light of current theories of the origin of B chromosomes.Key words: B chromosome, Secale cereale, repeated sequence, cloning, in situ hybridization.

Karyotype polymorphism in Drosophila albomicans

Genome ◽  
1987 ◽  
Vol 29 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Machiko Hatsumi
Keyword(s):  
Key Words ◽  
Secondary Constriction ◽  
B Chromosome ◽  
B Chromosomes ◽  
Chromosome 4 ◽  
Isofemale Lines ◽  
Heterochromatic Segment ◽  
Drosophila Albomicans ◽  
Karyotype Polymorphism

Supernumerary (B) chromosomes are present in Thai, Malay, and Burmese populations of Drosophila albomicans (2n = 6) in a polymorphic state. Although usually stable at mitosis, their numbers differed between individuals and their frequency was also different between isofemale lines and between populations. Arm 3 of the X3 chromosome was polymorphic for the presence and the size of a procentric heterochromatic segment. Chromosome 4 is polytypic for variation in length governed by differences in the amount of heterochromatin and the long variant is polymorphic for the location of its secondary constriction. Key words: Drosophila albomicans, karyotype polymorphism, B chromosome.

scholarly journals CHROMOSOME VARIATION IN CALLUS CELLS DERIVED FROM SECALE CEREALE L. WITH AND WITHOUT B-CHROMOSOME

1976 ◽  
Vol 51 (5) ◽  
pp. 297-303 ◽  
Author(s):  
HITOSHI ASAMI ◽  
NOBUMICHI INOMATA ◽  
MASASUKE OKAMOTO
Keyword(s):  
Secale Cereale ◽  
B Chromosome ◽  
Chromosome Variation ◽  
Secale Cereale L

scholarly journals Comparison of B chromosome effects on Secale cereale and Secale vavilovii

Heredity ◽  
1985 ◽  
Vol 55 (2) ◽  
pp. 229-234 ◽  
Author(s):  
M J Puertas ◽  
F Romera ◽  
A de la Peña
Keyword(s):  
Secale Cereale ◽  
B Chromosome

Molecular cytogenetic analysis of supernumerary heterochromatic segments in Rumex acetosa

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 391-397 ◽  
Author(s):  
Fukashi Shibata ◽  
Masahiro Hizume ◽  
Yuzo Kuroki
Keyword(s):  
In Situ Hybridization ◽  
Copy Number ◽  
Tandem Repeats ◽  
Mitotic Chromosomes ◽  
Dioecious Plant ◽  
Rumex Acetosa ◽  
Interphase Nuclei ◽  
Heterochromatic Segment ◽  
Molecular Cytogenetic

The dioecious plant Rumex acetosa shows intraspecific karyotype variation, caused by supernumerary heterochromatic segments or DAPI (4',6-diamidino-2 phenylindole)-bands at the ends of the short arms of three pairs of autosomes. A DNA sequence (RAE730) specific to the supernumerary heterochromatic segments was cloned and sequenced. RAE730 was about 730 bp and AT-rich (71% AT-content). Using fluorescence in situ hybridization (FISH), RAE730 was localized in the supernumerary DAPI-positive heterochromatic segments on several mitotic chromosomes and chromocenters in interphase nuclei, but not in the DAPI-bands of Y or B chromosomes. RAE730 was tandemly arranged in the genome, and the copy number varied between plants from 40 000 to 304 000 copies per 2C, corresponding to the relative amount of supernumerary heterochromatic segments per genome. These results indicate that the karyotype variation caused by the supernumerary heterochromatic segment was generated by amplification or reduction of the tandem repeats of RAE730. Key words: Rumex acetosa, repetitive sequence, supernumerary heterochromatic segment, intraspecific karyotype variation, DAPI-band.

Clinical and molecular cytogenetic characterization of two cases of inverted duplication deletion 8p

2020 ◽  
pp. 41-42
Author(s):  
Д.А. Юрченко ◽  
М.Е. Миньженкова ◽  
Е.Л. Дадали ◽  
Н.В. Шилова
Keyword(s):  
Mental Retardation ◽  
Congenital Heart ◽  
Heart Defects ◽  
Clinical Manifestations ◽  
Formation Mechanisms ◽  
Agenesis Of Corpus Callosum ◽  
Molecular Cytogenetic ◽  
Inverted Duplication ◽  
Cytogenetic Characterization

Синдром инвертированной дупликации короткого плеча хромосомы 8 со смежной терминальной делециенй (inv dup del(8p), ORPHA 96092) - редкая хромосомная аномалия (ХА) с частотой 1/10000-1/30000 живорожденных. В статье представлены клинические и молекулярно-цитогенетические характеристики двух неродственных пациентов с синдромом inv dup del(8p) и уточнены механизмы формирования хромосомного дисбаланса. Inverted duplication deletion 8p syndrome (inv dup del(8p), ORPHA 96092) is a rare chromosomal abnormality with a frequency of 1:10,000 - 30,000 newborns. Clinical manifestations of this syndrome include mental retardation, facial anomalies, hypoplasia/agenesis of corpus callosum, scoliosis and/or kyphosis, hypotonia, congenital heart defects. The article presents the clinical and molecular cytogenetic characteristics of two patients with inv dup del (8p) syndrome and clarifies the formation mechanisms.

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