Molecular cytogenetic evaluation of chromosome instability inTriticum aestivum—Secale cereale disomic addition lines

2010 ◽  
Vol 51 (2) ◽  
pp. 149-152 ◽  
Author(s):  
É. Szakács ◽  
M. Molnár-Láng
Keyword(s):  
Secale Cereale ◽  
Chromosome Instability ◽  
Addition Lines ◽  
Molecular Cytogenetic ◽  
Disomic Addition Lines

scholarly journals Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.)

Genome ◽  
2016 ◽  
Vol 59 (3) ◽  
pp. 159-165 ◽  
Author(s):  
Ling Qiu ◽  
Zong-xiang Tang ◽  
Meng Li ◽  
Shu-lan Fu
Keyword(s):  
Secale Cereale ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Addition Lines ◽  
Breeding Programs ◽  
Effective Utilization ◽  
Secale Cereale L ◽  
Monosomic Addition ◽  
Monosomic Addition Lines ◽  
Disomic Addition Lines

PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat–rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. ‘Mianyang11’ × S. cereale L. ‘Kustro’. In addition, two sets of wheat–rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring × S. cereale L. var. Imperial and T. aestivum L. ‘Holdfast’ × S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat–rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs.

Molecular Cytogenetic Characterization of Eb‐Genome Chromosomes in Thinopyrum bessarabicum Disomic Addition Lines of Bread Wheat

2002 ◽  
Vol 163 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Ji‐Yi Zhang ◽  
Xiao‐Mei Li ◽  
Richard R.‐C. Wang ◽  
A. Cortes ◽  
V. Rosas ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Addition Lines ◽  
Molecular Cytogenetic ◽  
Cytogenetic Characterization ◽  
Disomic Addition Lines ◽  
Thinopyrum Bessarabicum

scholarly journals The Y chromosome disomy syndrome (47, XYY) in children with mental retardation, deviations of sex development and different genome anomalies: molecular cytogenetic studies

2020 ◽  
Vol 65 (2) ◽  
pp. 40-48
Author(s):  
S. G. Vorsanova ◽  
I. V. Solovyev ◽  
O. S. Kurinnaya ◽  
V. S. Kravets ◽  
A. D. Kolotii ◽  
...  
Keyword(s):  
Mental Retardation ◽  
Y Chromosome ◽  
Clinical Symptoms ◽  
Chromosome Instability ◽  
Psychomotor Retardation ◽  
Common Mechanism ◽  
Chromosomal Anomalies ◽  
Molecular Cytogenetic ◽  
Sex Development

The article present the results of retrospectively analyzed children (4424 boys) with mental and psychomotor retardation, congenital malformations and/or developmental micro anomalies. 23 children had various forms of Y chromosome dysomy syndrome. The frequency of this syndrome in the studied cohort was 0.52%; and in this connection the authors discussed the role of Y-chromosome in the origin of mental retardation. Besides, the chromosome instability in sex and somatic cells is supposed to be a common mechanism of different chromosomal anomalies. The authors discussed the possibility of cytogenetic and molecular cytogenetic diagnosis, and also clinical polymorphism of the syndrome. The authors established the necessity of molecular cytogenetic technologies in the diagnosis of different forms of the syndrome, including mosaic forms and isodicentric chromosomes-connected forms. The severity of clinical symptoms doesn’t depend on presence of regular or mosaic forms of the syndrome. The study assumes a possible connection of clinical polymorphism with mosaisism, associated with the presence of abnormal cells (cell lines) in different tissues, together with the role of Y chromosome in the origin of mental retardation in children with Y- chromosome disomy syndrome and other chromosomal anomalies. The authors underline the necessity of molecular cytogenetic diagnosis of different forms of the syndrome for correct medical and genetic consultation.

Chromosome Deletion and Translocation in Chinese Spring-Lophopyrum Elongatum6E Disomic Addition Lines Induced by the Gametocidal Chromosome 2C fromAegilops Cylindrica

2013 ◽  
Vol 27 (2) ◽  
pp. 3638-3643 ◽  
Author(s):  
Hai-Bin Zhao ◽  
Li-Juan Zhao ◽  
Li-Wei Zhao ◽  
Hong-Mei Yuan ◽  
Yan-Ming Zhang ◽  
...  
Keyword(s):  
Chinese Spring ◽  
Addition Lines ◽  
Chromosome Deletion ◽  
Disomic Addition Lines

Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 893-905 ◽  
Author(s):  
M Kubaláková ◽  
M Valárik ◽  
J Bartoš ◽  
J Vrána ◽  
J Cíhalíková ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Physical Mapping ◽  
Secale Cereale ◽  
B Chromosomes ◽  
Addition Lines ◽  
Large Numbers ◽  
Secale Cereale L

Procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) were developed for rye (Secale cereale L.). Suspensions of intact chromosomes were prepared by mechanical homogenization of synchronized root tips after mild fixation with formaldehyde. Histograms of relative fluorescence intensity obtained after the analysis of DAPI-stained chromosomes (flow karyotypes) were characterized and the chromosome content of the DNA peaks was determined. Chromosome 1R could be discriminated on a flow karyotype of S. cereale 'Imperial'. The remaining rye chromosomes (2R–7R) could be discriminated and sorted from individual wheat–rye addition lines. The analysis of lines with reconstructed karyotypes demonstrated a possibility of sorting translocation chromosomes. Supernumerary B chromosomes could be sorted from an experimental rye population and from S. cereale 'Adams'. Flow-sorted chromosomes were identified by fluorescence in situ hybridization (FISH) with probes for various DNA repeats. Large numbers of chromosomes of a single type sorted onto microscopic slides facilitated detection of rarely occurring chromosome variants by FISH with specific probes. PCR with chromosome-specific primers confirmed the identity of sorted fractions and indicated suitability of sorted chromosomes for physical mapping. The possibility to sort large numbers of chromosomes opens a way for the construction of large-insert chromosome-specific DNA libraries in rye.Key words: chromosome isolation, chromosome sorting, fluorescence in situ hybridization, repetitive DNA sequences, wheat-rye addition lines, B chromosomes, physical mapping.

scholarly journals Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes

2020 ◽  
Vol 21 (11) ◽  
pp. 4053
Author(s):  
Liqiang Song ◽  
Hui Zhao ◽  
Zhi Zhang ◽  
Shuai Zhang ◽  
Jiajia Liu ◽  
...  
Keyword(s):  
Addition Line ◽  
Disomic Addition Line ◽  
Molecular Cytogenetic ◽  
Exploitation And Utilization ◽  
Specific Locus ◽  
Domestication Process ◽  
Disomic Addition Lines ◽  
M Genome ◽  
Black Color

Production of wheat-alien disomic addition lines is of great value to the exploitation and utilization of elite genes originated from related species to wheat. In this study, a novel wheat-Aegilops biuncialis 5Mb disomic addition line WA317 was characterized by in situ hybridization (ISH) and specific-locus amplified fragment sequencing (SLAF-seq) markers. Compared to its parent Chinese Spring (CS), the glumes of WA317 had black color and were difficult to remove after harvesting, suggesting chromosome 5Mb carried gene(s) related to glume development and Triticeae domestication process. A total of 242 Ae. biuncialis SLAF-based markers (298 amplified patterns) were developed and further divided into four categories by Ae. biuncialis Y17, Ae. umbellulata Y139 and Ae. comosa Y258, including 172 markers amplifying the same bands of U and M genome, six and 102 markers amplifying U-specific and M-specific bands, respectively and eighteen markers amplifying specific bands in Y17. Among them, 45 markers had the specific amplifications in WA317 and were 5Mb specific markers. Taken together, line WA317 with tenacious and black glumes should serve as the foundation for understanding of the Triticeae domestication process and further exploitation of primitive alleles for wheat improvement. Ae. biuncialis SLAF-based markers can be used for studying syntenic relationships between U and M genomes as well as rapid tracking of U and M chromosomal segments in wheat background.

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