Development of a high-throughput yeast two-hybrid screening system to study protein-protein interactions in plants

2002 ◽  
Vol 267 (2) ◽  
pp. 142-153 ◽  
Author(s):  
Y. Fang ◽  
D. Macool ◽  
Z. Xue ◽  
E. Heppard ◽  
C. Hainey ◽  
...  
Keyword(s):  
High Throughput ◽  
Protein Interactions ◽  
Screening System ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Two Hybrid ◽  
Hybrid Screening

The yeast two-hybrid screening technique and its use in the study of protein-protein interactions in apoptosis

1998 ◽  
Vol 10 (2) ◽  
pp. 131-136 ◽  
Author(s):  
David Wallach ◽  
Mark P Boldin ◽  
Andrei V Kovalenko ◽  
Nikolai L Malinin ◽  
Igor L Mett ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Screening Technique ◽  
Two Hybrid ◽  
Hybrid Screening

scholarly journals High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions

2011 ◽  
Vol 81A (1) ◽  
pp. 90-98 ◽  
Author(s):  
Jun Chen ◽  
Mark B. Carter ◽  
Bruce S. Edwards ◽  
Hong Cai ◽  
Larry A. Sklar
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Protein Interactions ◽  
Large Scale ◽  
Scale Analysis ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Large Scale Analysis ◽  
Two Hybrid

Abstract 1946: Detecting protein-protein interactions between MUC16 CDΔ102 (80AA) and a SKOV-3 cDNA library using yeast two-hybrid screening

2011 ◽  
Author(s):  
Stephan Polterauer ◽  
Ryan Parlett ◽  
Dharmarao Thapi ◽  
Nestor Rosales ◽  
David R. Spriggs ◽  
...  
Keyword(s):  
Cdna Library ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Two Hybrid ◽  
Hybrid Screening

scholarly journals Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm

Yeast ◽  
2000 ◽  
Vol 1 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Albertha J. M. Walhout ◽  
Simon J. Boulton ◽  
Marc Vidal
Keyword(s):  
Hybrid Systems ◽  
High Throughput ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Large Scale ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Systematic Analysis ◽  
Technical Features ◽  
Two Hybrid

The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as ‘functional genomics’. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein–protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein–protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeastSaccharomyces cerevisiaeand the nematodeCaenorhabditis elegans.

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