Towards an Ideal In Cell Hybridization-Based Strategy to Discover Protein Interactomes of Selected RNA Molecules

RNA-binding proteins are crucial to the function of coding and non-coding RNAs. The disruption of RNA–protein interactions is involved in many different pathological states. Several computational and experimental strategies have been developed to identify protein binders of selected RNA molecules. Amongst these, ‘in cell’ hybridization methods represent the gold standard in the field because they are designed to reveal the proteins bound to specific RNAs in a cellular context. Here, we compare the technical features of different ‘in cell’ hybridization approaches with a focus on their advantages, limitations, and current and potential future applications.

Effect of Chitosan Deacetylation on Its Affinity to Type III Collagen: A Molecular Dynamics Study

The ability to form strong intermolecular interactions by linear glucosamine polysaccharides with collagen is strictly related to their nonlinear dynamic behavior and hence bio-lubricating features. Type III collagen plays a crucial role in tissue regeneration, and its presence in the articular cartilage affects its bio-technical features. In this study, the molecular dynamics methodology was applied to evaluate the effect of deacetylation degree on the chitosan affinity to type III collagen. The computational procedure employed docking and geometry optimizations of different chitosan structures characterized by randomly distributed deacetylated groups. The eight different degrees of deacetylation from 12.5% to 100% were taken into account. We found an increasing linear trend (R2 = 0.97) between deacetylation degree and the collagen–chitosan interaction energy. This can be explained by replacing weak hydrophobic contacts with more stable hydrogen bonds involving amino groups in N-deacetylated chitosan moieties. In this study, the properties of chitosan were compared with hyaluronic acid, which is a natural component of synovial fluid and cartilage. As we found, when the degree of deacetylation of chitosan was greater than 0.4, it exhibited a higher affinity for collagen than in the case of hyaluronic acid.

Computer Simulations of Deep Eutectic Solvents: Challenges, Solutions, and Perspectives

Deep eutectic solvents (DESs) are one of the most rapidly evolving types of solvents, appearing in a broad range of applications, such as nanotechnology, electrochemistry, biomass transformation, pharmaceuticals, membrane technology, biocomposite development, modern 3D-printing, and many others. The range of their applicability continues to expand, which demands the development of new DESs with improved properties. To do so requires an understanding of the fundamental relationship between the structure and properties of DESs. Computer simulation and machine learning techniques provide a fruitful approach as they can predict and reveal physical mechanisms and readily be linked to experiments. This review is devoted to the computational research of DESs and describes technical features of DES simulations and the corresponding perspectives on various DES applications. The aim is to demonstrate the current frontiers of computational research of DESs and discuss future perspectives.

Research on Application of Wireless Bridge Technology in the Smart Grid

The construction of smart grid, in the meet of communication network core layer must have the strong bearing capacity and the strong network frame, also requires the access layer have the wide variety and flexible edge access ability. In this article, in accordance with the technical features of the smart grid communication network, combined with the Global Energy Interconnection to the requirement of all kinds of communication. The wireless bridge system based on the IEEE 802.11 series protocol as flexible edge access is applied to the smart grid communications, to forward the power wireless bridge structure design and the bridge monitoring management platform design and safety protection design. The use of a wireless bridge will increase the transmission quality of signal, distance and the safety of the transmission. In this article, the wireless bridge in the application of the smart grid electric power communication network has a role in promoting, has a guiding significance to the construction of the smart grid communication.

Techno-economic aspects and problems of the rotary rowing units introduction in the modernization and construction of ships (on the example of projects 1809 and CNF11CPD)

The article contains a detailed analysis of studies related to the use of existing and new principles of fuel economy, and reducing the cost of re-equipment and operation of vessels of the above projects. The authors of the article did not set out to analyze the technical features of these projects. Only by linking to these projects, the analysis was done for the entire fleet of this type as a whole. A technical and economic analysis of the characteristics, modeling of the main and auxiliary installations in the Matlab package, providing fuel economy on the example of ships of projects 1809 and CNF11CPD, is carried out. Then a physical simulation was carried out. As a result of testing a vessel with a rotary rowing electric installation (RSEU), a qualitative analysis of the data obtained was carried out, conclusions were drawn that allow us to judge the possibility of large-scale use of such installations in maritime transport.

Analysis of Some Essential Aspects Related to the Navigation Conditions on the Danube River

The European Union has emphasized the creation of an appropriate framework to optimize the internal market and inland transport waterways, and remove barriers to their wider use. Administrative barriers in the logistics of goods on the Danube waterway and its navigable tributaries constitute a significant obstacle to the efficient and sustainable use of the Danube as the region’s central transport hub. The approach proposed in this paper was designed to identify and analyze the relationship between the main variables leading to problematic inland waterway traffic, in this case, on the Danube, and the measures taken by the European Commission to improve it. In terms of the applied research method, “Quality Function Deployment” (QFD), we assign global (overall) and local priority degrees. The proposed framework for adapting QFD as a tool for improving quality and, therefore, performance, aims to identify and prioritize directions for this improvement. The House of Quality (HOQ) is the tool that links areas for improvement to technical requirements. The disclosure of these connections helps identify and prioritize the technical features that will generate the most significant improvements.

An integrated automatic aviation meteorological instrument system at C. S. I. airport, Mumbai

An ‘Integrated Automatic Aviation Meteorological Instrument System’ (IAAMS) is installed atMumbai International Airport in May-2008. The system is State-of-the-Art and has features like automatic generation ofaviation reports; METAR / SPECI, AFTN (Aeronautical Fixed Telecommunication Network) connectivity fordissemination of aviation weather reports to user agencies and ATIS (Automatic Terminal Interface System) forautomatic broadcasting of aviation weather reports to airborne pilot. Besides these features system is having facility toenter manually weather parameter for which sensors are not available such as cloud coverage, past weather, presentweather for generation of aviation reports. The system meets the requirements of operational desirable accuracy ofInternational Civil Aviation Organization (ICAO, 2004) and vector averaging of winds as per World MeteorologicalOrganization guidelines (WMO, 1992). The paper is mainly focusing on the different technical features of this newsystem along with its observational response in last one year at Mumbai airport. Similar system has been installed at othermajor airports; New Delhi (4 sites), Chennai, Hyderabad, Bangalore, Amritsar, Jaipur, and Guwahati. The system issupplied and installed by Telvent Company, Australia.

New Generation of Smart Highway: Framework and Insights

The new generation of smart highway (NGSH) has become an irresistible global trend to improve transport efficiency and safety. The exploration of the features and framework for NGSH can guide us to upgrade the current highway system. This paper summarizes the fundamental features of the NGSH from the perspective of the interactive evolution of automobile industry and road transport. In line with the popularity of automated and connected vehicles, the primary technical features of the NGSH are proposed as (I) complete elements sensing, (II) cyber-physical systems, (III) cooperative vehicle-infrastructure applications, and (IV) 5th generation mobile communication technology. The corresponding physical framework and data flow are introduced, in which three data attributes (data accuracy, dimensionality, and freshness) are highlighted to describe the data requirements for various scenarios. The development path of the NGSH is further discussed in terms of the different vehicle automation levels. The characteristics of five levels of NGSH are identified from R1 to R5. Different combinations of NGSH level and vehicle automation level lead to distinct system functions. Several urgent problems in the current stage are pointed out in terms of system compatibility, standard specification, and information security. This paper provides new insights for sustainable and reproducible highway reformation, drawing some implications for NGSH design.

ANALYSIS OF EXISTING SOLUTIONS FOR IMPROVING THE MEASURING AND COMPUTING COMPLEXES OF HEAT SUPPLY NETWORKS

In the presented article, the team of authors considers the existing methods and the main modern technical solutions that are currently implemented in different countries in the diagnosis of heat supply networks. There is a selection of the main directions in the development and design of heat supply networks, which have already been implemented or supported by scientific teams from different countries. Various methods and technical features of diagnostics are reviewed, strengths and weaknesses of the presented solutions are highlighted. The reviewed works were subjected to detailed analysis, which revealed the presence of a high interest of the scientific and industrial community in the integration and improvement of existing digital technologies in the development of heat supply systems, which would be closely related to forecasting and modeling processes in this industry. The team of authors highlights the main vectors for the development of this sector, citing an example of a significant increase in the degree of digitalization of final products, which makes it possible to use data analytics to obtain effective technical solutions regarding heat supply networks. Separately, the positive experience of different countries in this industry is noted when using neural networks not only in the design of heat supply networks, but also as a target industry as a whole. Assumptions are put forward about the need for a detailed analysis of the existing foreign and domestic experience, as well as scientific developments in this area, in order to determine the most suitable technical solutions on the territory of the Russian Federation, which will take into account the climatic characteristics of the country and be based on methods of large data analysis, computer vision and simulation. modeling.

Quality improvement of the e-commerce website using integration of Kano model-ipa with QFD approach

This study was conducted to analyze the quality of e-commerce websites, find out which items need improvement, and make improvement design e-commerce websites. This study uses the kano model and Importance Performance Analysis approach based on 7 dimensions with 39 attributes. This study used the survey results from 103 respondents who regularly use the e-commerce website Tokopedia.com. The results of the questionnaire through the validity and reliability tests were used to analyze the reliability of items and the feasibility of the results of the questionnaire. After that, the results of the questionnaire through the corellation and hypothesis tests were used to know the relationship between variables. The findings show that 7 attributes influence customer satisfaction. However, the findings are still nowhere near the expectations. Thus, it requires being the focus of improvement. Improvements in design of 7 attributes in the form of House of Quality with 6 technical features needed to improve the quality of e-commerce website Tokopedia.com. Therefore, effective management strategies can be applied to overcome the intense competition in the e-commerce industry.