scholarly journals Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts

2004 ◽  
Vol 315 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Stefan Hamm ◽  
B�n�dicte Dehouck ◽  
J�rg Kraus ◽  
Karen Wolburg-Buchholz ◽  
Hartwig Wolburg ◽  
...  
Keyword(s):  
Tight Junction ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Tight Junction Proteins ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Blood Brain ◽  
Brain Barrier Permeability ◽  
Junction Proteins

Tight Junction Regulation by Morphine and HIV-1 Tat Modulates Blood–Brain Barrier Permeability

2008 ◽  
Vol 28 (5) ◽  
pp. 528-541 ◽  
Author(s):  
Supriya D. Mahajan ◽  
Ravikumar Aalinkeel ◽  
Donald E. Sykes ◽  
Jessica L. Reynolds ◽  
B. Bindukumar ◽  
...  
Keyword(s):  
Tight Junction ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Blood Brain ◽  
Brain Barrier Permeability ◽  
Tight Junction Regulation ◽  
Hiv 1

scholarly journals Maternal glucocorticoid exposure alters tight junction protein expression in the brain of fetal sheep

2010 ◽  
Vol 298 (1) ◽  
pp. H179-H188 ◽  
Author(s):  
Grazyna B. Sadowska ◽  
Shadi N. Malaeb ◽  
Barbara S. Stonestreet
Keyword(s):  
Spinal Cord ◽  
Cerebral Cortex ◽  
Protein Expression ◽  
Tight Junction ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Blood Brain ◽  
Brain Barrier Permeability

We examined the expression of tight junction (TJ) proteins in the cerebral cortex, cerebellum, and spinal cord of fetuses after maternal treatment with single and multiple courses of dexamethasone. Ewes received either single courses of four 6-mg dexamethasone or placebo injections every 12 h for 48 h between 104 and 107 days or the same treatment once a week between 76–78 and 104–107 days of gestation. TJ protein expression was determined by Western immunoblot analysis on tissue harvested at 105–108 days of gestation. Blood-brain barrier permeability has been previously quantified with the blood-to-brain transfer constant ( Ki) with α-aminoisobutyric acid ( 39 ). After a single course of dexamethasone, claudin-5 increased ( P < 0.05) in the cerebral cortex, occludin and claudin-1 increased in the cerebellum, and occludin increased in the spinal cord. After multiple dexamethasone courses, occludin and zonula occludens (ZO)-1 increased in the cerebral cortex, and occludin and claudin-1 increased in the cerebellum. Junctional adhesion molecule-A and ZO-2 expressions did not change. Linear regression comparing Ki to TJ proteins showed inverse correlations with claudin-1 and claudin-5 in the cerebral cortex after a single course and ZO-2 in the spinal cord after multiple courses and direct correlations with ZO-1 in the cerebellum and spinal cord after multiple courses. We conclude that maternal glucocorticoid treatment increases the expression of specific TJ proteins in vivo, patterns of TJ protein expression vary after exposure to single and multiple glucocorticoid courses, and decreases in blood-brain barrier permeability are associated with increases in claudin-1, claudin-5, and ZO-2 expression and decreases in ZO-1 expression. In utero glucocorticoid exposure alters the molecular composition of the barrier and affects fetal blood-brain barrier function.

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