Erratum to: Characterization and differentiation potential of rat ventral mesencephalic neuronal progenitor cells immortalized with SV40 large T antigen

Abstract We describe the development of a human bone marrow (BM) culture system which allows study of the interaction of stromal cell lines (SCL) and highly purified hematopoietic progenitor cells. Normal BM stromal cells were electroporated with a plasmid containing the simian virus 40 (SV40) large T antigen (SV40 T Ag) under the control of a synthetic metallothionein promoter (MT4); this construct is designated MT4 SV40 T Ag. SCL in which the rate of proliferation could be controlled by altering the zinc (Zn) concentration were characterized, demonstrating that the SCL were heterogeneous with respect to G-CSF and GM-CSF production. Suppression of SCL proliferation on removal of Zn made it possible to use these lines in coculture with purified CD34+ progenitor cells from umbilical cord blood. The ability to control proliferation of SCL has allowed us to maintain the survival and expansion of colony- forming cells in culture for up to 2 months. These lines have enabled us to test for stromal cell characteristics at a clonal level and provided us with a tool to analyze the events leading to lineage commitment and hematopoietic differentiation, as demonstrated by suppression of hematopoiesis by an antibody directed against the c-kit molecule.

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Conditioned medium from Endothelial Progenitor Cells promotes number of dopaminergic neurons and exerts neuroprotection in cultured ventral mesencephalic neuronal progenitor cells

Brain Research ◽

10.1016/j.brainres.2019.146330 ◽

2019 ◽

Vol 1720 ◽

pp. 146330 ◽

Cited By ~ 2

Author(s):

Stefano Di Santo ◽

Stefanie Seiler ◽

Angélique D. Ducray ◽

Hans Rudolf Widmer

Keyword(s):

Progenitor Cells ◽

Conditioned Medium ◽

Endothelial Progenitor Cells ◽

Dopaminergic Neurons ◽

Endothelial Progenitor ◽

Neuronal Progenitor Cells ◽

Neuronal Progenitor ◽

Ventral Mesencephalic

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Support of human cord blood progenitor cells on human stromal cell lines transformed by SV40 large T antigen under the influence of an inducible (metallothionein) promoter

Blood ◽

10.1182/blood.v80.1.102.bloodjournal801102 ◽

1992 ◽

Vol 80 (1) ◽

pp. 102-112

Author(s):

FM Cicuttini ◽

M Martin ◽

E Salvaris ◽

L Ashman ◽

CG Begley ◽

...

Keyword(s):

Cord Blood ◽

Progenitor Cells ◽

Cell Lines ◽

Stromal Cell ◽

Simian Virus 40 ◽

T Antigen ◽

Large T Antigen ◽

Sv40 Large T Antigen ◽

Human Cord Blood ◽

Metallothionein Promoter

We describe the development of a human bone marrow (BM) culture system which allows study of the interaction of stromal cell lines (SCL) and highly purified hematopoietic progenitor cells. Normal BM stromal cells were electroporated with a plasmid containing the simian virus 40 (SV40) large T antigen (SV40 T Ag) under the control of a synthetic metallothionein promoter (MT4); this construct is designated MT4 SV40 T Ag. SCL in which the rate of proliferation could be controlled by altering the zinc (Zn) concentration were characterized, demonstrating that the SCL were heterogeneous with respect to G-CSF and GM-CSF production. Suppression of SCL proliferation on removal of Zn made it possible to use these lines in coculture with purified CD34+ progenitor cells from umbilical cord blood. The ability to control proliferation of SCL has allowed us to maintain the survival and expansion of colony- forming cells in culture for up to 2 months. These lines have enabled us to test for stromal cell characteristics at a clonal level and provided us with a tool to analyze the events leading to lineage commitment and hematopoietic differentiation, as demonstrated by suppression of hematopoiesis by an antibody directed against the c-kit molecule.

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