Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids

1994 ◽  
Vol 278 (3) ◽  
pp. 611-616
Author(s):  
Jean-Pierre Dadoune ◽  
Jean-Pierre Siffroi ◽  
Marie-Fran�oise Alfonsi
Keyword(s):  
In Situ Hybridization ◽  
Ultrastructural Localization

scholarly journals Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan

2005 ◽  
Vol 36 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Wun-Ju Shieh ◽  
Cheng-Hsiang Hsiao ◽  
Christopher D. Paddock ◽  
Jeannette Guarner ◽  
Cynthia S. Goldsmith ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Severe Acute Respiratory Syndrome ◽  
Fatal Case ◽  
Ultrastructural Localization

Ultrastructural localization of the RNA of immunodeficiency viruses using electron microscopy in situ hybridization and in vitroinfected lymphocytes

Micron ◽  
2001 ◽  
Vol 32 (6) ◽  
pp. 579-589 ◽  
Author(s):  
C. Cantó-Nogués ◽  
D. Hockley ◽  
C. Grief ◽  
S. Ranjbar ◽  
J. Bootman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
In Situ Hybridization ◽  
Ultrastructural Localization

scholarly journals Ultrastructural localization of herpes simplex virus RNA by in situ hybridization.

1989 ◽  
Vol 37 (1) ◽  
pp. 97-104 ◽  
Author(s):  
R A Wolber ◽  
T F Beals ◽  
H F Maassab
Keyword(s):  
In Situ Hybridization ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Ultrastructural Localization ◽  
Dna Probe ◽  
Gold Particles ◽  
Virus Rna ◽  
Dnase Treatment ◽  
Simplex Virus

We studied the subcellular localization of virally encoded RNA by pre-embedding in situ hybridization, using colloidal gold as an electron-dense marker. Fibroblasts infected with Herpes simplex virus (HSV) were fixed, permeabilized, then hybridized with a biotinylated HSV DNA probe under conditions favoring DNA-RNA hybrid formation. HSV probe was localized with 5-nm streptavidin-gold conjugates. Transmission electron microscopy revealed 5-nm gold in clusters and singlets within HSV-infected cells. Formalin-fixed cells contained a mean of 4.6 clusters per cytoplasmic profile and 13.2 clusters per nuclear profile. Combined formalin-glutaraldehyde fixation increased the mean number of clusters per cytoplasmic and nuclear profile to 7.2 (57% increase) and 17.5 (33% increase), respectively. Gold clusters were frequently located in regions adjacent to the nuclear envelope but were not bound to viral nucleocapsids or endoplasmic reticulum. Labeling was unaffected by pre-hybridization DNAse treatment of cells. RNAse eliminated 87% of cytoplasmic and 97% of nuclear clusters. These findings indicate that clustered gold particles labeled viral RNA, with probable binding of multiple DNA probe molecules and/or gold particles to RNA strands. This novel pre-embedding technique may be a useful tool for ultrastructural evaluation of virus-host cell interactions.

Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids

1994 ◽  
Vol 278 (3) ◽  
pp. 611-616 ◽  
Author(s):  
Jean-Pierre Dadoune ◽  
Jean-Pierre Siffroi ◽  
Marie-Fran�oise Alfonsi
Keyword(s):  
In Situ Hybridization ◽  
Ultrastructural Localization

Ultrastructural Localization of Human Papillomavirus Sequences by In Situ Hybridization and Image Analysis

1998 ◽  
Vol 21 (4) ◽  
pp. 295-298 ◽  
Author(s):  
A. Picazo ◽  
F. Gomez ◽  
M. T. Corcuera ◽  
M. Roldan ◽  
C. Hoyos ◽  
...  
Keyword(s):  
Image Analysis ◽  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Ultrastructural Localization

Arginine-Vasopressin in Anterior Pituitary Cells: In situ Hybridization of mRNA and Ultrastructural Localization of Immunoreactivity

1991 ◽  
Vol 54 (3) ◽  
pp. 303-311 ◽  
Author(s):  
Chantal Terrier ◽  
Jean-Guy Chabot ◽  
Gerard Pautrat ◽  
Lydie Jeandel ◽  
Dave Gray ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Arginine Vasopressin ◽  
Anterior Pituitary ◽  
Ultrastructural Localization ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells
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