scholarly journals Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan

2005 ◽  
Vol 36 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Wun-Ju Shieh ◽  
Cheng-Hsiang Hsiao ◽  
Christopher D. Paddock ◽  
Jeannette Guarner ◽  
Cynthia S. Goldsmith ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Severe Acute Respiratory Syndrome ◽  
Fatal Case ◽  
Ultrastructural Localization

Ultrastructural localization of the RNA of immunodeficiency viruses using electron microscopy in situ hybridization and in vitroinfected lymphocytes

Micron ◽  
2001 ◽  
Vol 32 (6) ◽  
pp. 579-589 ◽  
Author(s):  
C. Cantó-Nogués ◽  
D. Hockley ◽  
C. Grief ◽  
S. Ranjbar ◽  
J. Bootman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
In Situ Hybridization ◽  
Ultrastructural Localization

scholarly journals A Possible Case of Vertical Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Newborn With Positive Placental In Situ Hybridization of SARS-CoV-2 RNA

2020 ◽  
Vol 9 (5) ◽  
pp. 636-639
Author(s):  
Ihab Alamar ◽  
Mohammad H Abu-Arja ◽  
Taryn Heyman ◽  
Drucilla J Roberts ◽  
Niyati Desai ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Severe Acute Respiratory Syndrome ◽  
Vertical Transmission ◽  
Infected Mother ◽  
Definitive Evidence ◽  
Pregnant Mothers ◽  
Unborn Fetus

Abstract Little is known about the effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the coronavirus disease 2019 (COVID-19) on pregnant mothers and their infants. Moreover, there is no definitive evidence that SARS CoV- 2 can be vertically transmitted from an infected mother to the unborn fetus.

scholarly journals Ultrastructural localization of herpes simplex virus RNA by in situ hybridization.

1989 ◽  
Vol 37 (1) ◽  
pp. 97-104 ◽  
Author(s):  
R A Wolber ◽  
T F Beals ◽  
H F Maassab
Keyword(s):  
In Situ Hybridization ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Ultrastructural Localization ◽  
Dna Probe ◽  
Gold Particles ◽  
Virus Rna ◽  
Dnase Treatment ◽  
Simplex Virus

We studied the subcellular localization of virally encoded RNA by pre-embedding in situ hybridization, using colloidal gold as an electron-dense marker. Fibroblasts infected with Herpes simplex virus (HSV) were fixed, permeabilized, then hybridized with a biotinylated HSV DNA probe under conditions favoring DNA-RNA hybrid formation. HSV probe was localized with 5-nm streptavidin-gold conjugates. Transmission electron microscopy revealed 5-nm gold in clusters and singlets within HSV-infected cells. Formalin-fixed cells contained a mean of 4.6 clusters per cytoplasmic profile and 13.2 clusters per nuclear profile. Combined formalin-glutaraldehyde fixation increased the mean number of clusters per cytoplasmic and nuclear profile to 7.2 (57% increase) and 17.5 (33% increase), respectively. Gold clusters were frequently located in regions adjacent to the nuclear envelope but were not bound to viral nucleocapsids or endoplasmic reticulum. Labeling was unaffected by pre-hybridization DNAse treatment of cells. RNAse eliminated 87% of cytoplasmic and 97% of nuclear clusters. These findings indicate that clustered gold particles labeled viral RNA, with probable binding of multiple DNA probe molecules and/or gold particles to RNA strands. This novel pre-embedding technique may be a useful tool for ultrastructural evaluation of virus-host cell interactions.

Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids

1994 ◽  
Vol 278 (3) ◽  
pp. 611-616 ◽  
Author(s):  
Jean-Pierre Dadoune ◽  
Jean-Pierre Siffroi ◽  
Marie-Fran�oise Alfonsi
Keyword(s):  
In Situ Hybridization ◽  
Ultrastructural Localization

scholarly journals Analysis of Deaths During the Severe Acute Respiratory Syndrome (SARS) Epidemic in Singapore: Challenges in Determining a SARS Diagnosis

2004 ◽  
Vol 128 (2) ◽  
pp. 195-204 ◽  
Author(s):  
Pek Yoon Chong ◽  
Paul Chui ◽  
Ai E. Ling ◽  
Teri J. Franks ◽  
Dessmon Y. H. Tai ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
In Situ Hybridization ◽  
Severe Acute Respiratory Syndrome ◽  
Forensic Medicine ◽  
Diffuse Alveolar Damage ◽  
Health Sciences ◽  
Atypical Pneumonia ◽  
Singapore General Hospital

Abstract Context.—An outbreak of severe acute respiratory syndrome (SARS), an infectious disease attributed to a novel coronavirus, occurred in Singapore during the first quarter of 2003 and led to 204 patients with diagnosed illnesses and 26 deaths by May 2, 2003. Twenty-one percent of these patients required admission to the medical intensive care unit. During this period, the Center for Forensic Medicine, Health Sciences Authority, Singapore, performed a total of 14 postmortem examinations for probable and suspected SARS. Of these, a total of 8 were later confirmed as SARS infections. Objective.—Our series documents the difficulties encountered at autopsy during the initial phases of the SARS epidemic, when the pattern of infection and definitive diagnostic laboratory criteria were yet to be established. Design.—Autopsies were performed by pathologists affiliated with the Center for Forensic Medicine, Health Sciences Authority, Singapore. Tissue was accessed and read at the Tan Tock Seng Hospital, Singapore, and at the Armed Forces Institute of Pathology, Washington, DC. Autopsy tissue was submitted to the Virology Department, Singapore General Hospital, for analysis, and in situ hybridization for the SARS coronavirus was carried out at the National Institute of Infectious Diseases, Tokyo, Japan. Results.—Thirteen of 14 patients showed features of diffuse alveolar damage. In 8 patients, no precipitating etiology was identified, and in all of these patients, we now have laboratory confirmation of coronavirus infection. Two of the 8 patients presented at autopsy as sudden unexpected deaths, while the remaining 6 patients had been hospitalized with varying lengths of stay in the intensive care unit. In 3 patients, including the 2 sudden unexpected deaths, in situ hybridization showed the presence of virally infected cells within the lung. In 4 of the 8 SARS patients, pulmonary thromboemboli were also recognized on gross examination, while one patient had marantic cardiac valvular vegetations. Conclusions.—It is unfortunate that the term atypical pneumonia has been used in conjunction with SARS. Although nonspecific by itself, the term does not accurately reflect the underlying dangers of viral pneumonia, which may progress rapidly to acute respiratory distress syndrome. We observed that the clinical spectrum of disease as seen in our autopsy series included sudden deaths. This is a worrisome finding that illustrates that viral diseases will have a spectrum of clinical presentations and that the diagnoses made for such patients must incorporate laboratory as well as clinical data.

scholarly journals Tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases

2004 ◽  
Vol 202 (2) ◽  
pp. 157-163 ◽  
Author(s):  
KF To ◽  
Joanna HM Tong ◽  
Paul KS Chan ◽  
Florence WL Au ◽  
Stephen SC Chim ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Severe Acute Respiratory Syndrome ◽  
Hybridization Study ◽  
Cellular Tropism

Ultrastructural Localization of Human Papillomavirus Sequences by In Situ Hybridization and Image Analysis

1998 ◽  
Vol 21 (4) ◽  
pp. 295-298 ◽  
Author(s):  
A. Picazo ◽  
F. Gomez ◽  
M. T. Corcuera ◽  
M. Roldan ◽  
C. Hoyos ◽  
...  
Keyword(s):  
Image Analysis ◽  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Ultrastructural Localization

scholarly journals Third‐trimester placentas of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)‐positive women: histomorphology, including viral immunohistochemistry and in‐situ hybridization

2020 ◽  
Vol 77 (6) ◽  
pp. 994-999 ◽  
Author(s):  
Marie C Smithgall ◽  
Xiaolin Liu‐Jarin ◽  
Diane Hamele‐Bena ◽  
Adela Cimic ◽  
Mirella Mourad ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Severe Acute Respiratory Syndrome ◽  
Third Trimester
Sign in / Sign up

Export Citation Format

Share Document