Mucosal repair and growth factors: recombinant human hepatocyte growth factor as an innovative therapy for inflammatory bowel disease

2005 ◽  
Vol 40 (10) ◽  
pp. 925-931 ◽  
Author(s):  
Akio Ido ◽  
Masatsugu Numata ◽  
Mayumi Kodama ◽  
Hirohito Tsubouchi
Keyword(s):  
Inflammatory Bowel Disease ◽  
Growth Factors ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Bowel Disease ◽  
Mucosal Repair ◽  
Innovative Therapy ◽  
Human Hepatocyte Growth Factor ◽  
Inflammatory Bowel ◽  
Hepatocyte Growth

Ameliorating effect of hepatocyte growth factor on inflammatory bowel disease in a murine model

2005 ◽  
Vol 288 (4) ◽  
pp. G729-G735 ◽  
Author(s):  
Koushi Oh ◽  
Yuji Iimuro ◽  
Masaharu Takeuchi ◽  
Yasufumi Kaneda ◽  
Tsuyoshi Iwasaki ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Bowel Disease ◽  
Interleukin 12 ◽  
Trinitrobenzene Sulfonic Acid ◽  
Intestinal Epithelial ◽  
T Helper 1 ◽  
Inflammatory Bowel ◽  
Hepatocyte Growth

Hepatocyte growth factor (HGF), a multifunctional cytokine, accelerates intestinal epithelial proliferation. We studied the effects of HGF in mice with trinitrobenzene sulfonic acid-induced colitis, which shows clinical and molecular resemblance to Crohn's disease. Mice with colitis repeatedly were transfected intramuscularly with human HGF cDNA. Weight, survival, histopathology, proinflammatory cytokine mRNAs, and leukocyte infiltration were assessed. Treatment with HGF cDNA induced tyrosine phosphorylation of intestinal c-Met/HGF receptors, inhibited apoptosis, and promoted mitosis in intestinal epithelial cells, accelerating intestinal epithelial restoration and suppressing inflammation. Transfection with HGF cDNA markedly suppressed intestinal mRNA expression of T-helper 1 cytokines such as interleukin-12 and -1β, interferon-γ, and tumor necrosis factor-α. Numbers of total and CD4-positive T cells, neutrophils, and myloperoxidase activity in intestinal epithelium were diminished by HGF gene transfer, which also prevented weight loss, and improved survival. HGF might prove useful for controlling inflammatory bowel disease.

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