Chemiluminescence Determination of Glucose, Fructose and their Mixture by the Stopped-Flow Mixing Technique

2003 ◽  
Vol 141 (1-2) ◽  
pp. 73-78 ◽  
Author(s):  
Tomás Pérez-Ruiz ◽  
Carmen Martínez-Lozano ◽  
Virginia Tomás ◽  
José Fenoll
Keyword(s):  
1993 ◽  
Vol 283 (1) ◽  
pp. 471-475 ◽  
Author(s):  
Lourdes de la Peña ◽  
Agustina Gómez-Hens ◽  
Dolores Pérez-Bendito

1977 ◽  
Vol 23 (6) ◽  
pp. 1033-1036 ◽  
Author(s):  
M D Joseph ◽  
D J Kasprzak ◽  
S R Crouch

Abstract A stopped-flow clinical analyzer is described that makes use of a reaction loop containing immobilized enzyme(s) for the determination of the analyte/substrate. The analyzer has been evaluated by determining glucose with immobilized glucose oxidase. The stopped-flow mixing system was constructed at a current cost of less than $500. The analyzer separates the enzymatic reaction from a followup, spectrophotometric indicator reaction. This separation allows the enzymatic reaction to be used in either a fixed-time, kinetic mode or in an equilibrium mode. Likewise, the indicator reaction can be used in either mode. Results for glucose in blood serum indicate that good precision and accuracy can be obtained.


The Analyst ◽  
2001 ◽  
Vol 126 (8) ◽  
pp. 1436-1439 ◽  
Author(s):  
Tomás Pérez-Ruiz ◽  
Carmen Martínez-Lozano ◽  
Virginia Tomás ◽  
José Fenol

The Analyst ◽  
1993 ◽  
Vol 118 (6) ◽  
pp. 707-710 ◽  
Author(s):  
Pilar Izquierdo ◽  
Agustina Gómez-Hens ◽  
Dolores Pérez-Bendito

2003 ◽  
Vol 485 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Tomás Pérez-Ruiz ◽  
Carmen Martı́nez-Lozano ◽  
Virginia Tomás ◽  
José Fenoll
Keyword(s):  

The Analyst ◽  
2000 ◽  
Vol 125 (12) ◽  
pp. 2372-2375 ◽  
Author(s):  
Tomás Pérez-Ruiz ◽  
Carmen Martínez-Lozano ◽  
Virginia Tomás ◽  
José Fenoll

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