Detection, discrimination and quantitation of 22 bluetongue virus serotypes using real-time RT-PCR with TaqMan MGB probes

2015 ◽  
Vol 160 (9) ◽  
pp. 2249-2258 ◽  
Author(s):  
Yufei Feng ◽  
Tao Yang ◽  
Qingyuan Xu ◽  
Encheng Sun ◽  
Junping Li ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr

Detection of the European ‘field’ strain of bluetongue virus serotype 6 by real-time RT-PCR

2010 ◽  
Vol 141 (1-2) ◽  
pp. 186-188 ◽  
Author(s):  
C.A. Batten ◽  
P.A. van Rijn ◽  
C.A.L. Oura
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Field Strain ◽  
Rt Pcr ◽  
Virus Serotype

scholarly journals Evaluating the most appropriate pooling ratio for EDTA blood samples to detect Bluetongue virus using real-time RT-PCR

2018 ◽  
Vol 217 ◽  
pp. 58-63 ◽  
Author(s):  
John Flannery ◽  
Paulina Rajko-Nenow ◽  
Hayley Hicks ◽  
Holly Hill ◽  
Simon Gubbins ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr ◽  
Blood Samples ◽  
Edta Blood

Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1

2007 ◽  
Vol 145 (2) ◽  
pp. 115-126 ◽  
Author(s):  
A.E. Shaw ◽  
P. Monaghan ◽  
H.O. Alpar ◽  
S. Anthony ◽  
K.E. Darpel ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Virus Genome ◽  
Genome Segment ◽  
Initial Evaluation ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Development and Evaluation of Real Time RT-PCR Assays for Detection and Typing of Bluetongue Virus

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163014 ◽  
Author(s):  
Sushila Maan ◽  
Narender Singh Maan ◽  
Manjunatha N. Belaganahalli ◽  
Abraham C. Potgieter ◽  
Vinay Kumar ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr ◽  
Pcr Assays

Use of real-time RT-PCR as a rapid molecular approach for differentiation of field and vaccine strains of bluetongue virus serotypes 2 and 9

2008 ◽  
Vol 22 (1) ◽  
pp. 38-46 ◽  
Author(s):  
Gabriella Elia ◽  
Giovanni Savini ◽  
Nicola Decaro ◽  
Vito Martella ◽  
Liana Teodori ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Molecular Approach ◽  
Rt Pcr

Development of a real-time RT-PCR assay based on primer–probe energy transfer for the detection of all serotypes of bluetongue virus

2010 ◽  
Vol 167 (2) ◽  
pp. 165-171 ◽  
Author(s):  
N. LeBlanc ◽  
T.B. Rasmussen ◽  
J. Fernández ◽  
C. Sailleau ◽  
L.D. Rasmussen ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Circulation of bluetongue virus in goats in the Karamoja region of Uganda

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Elijah N. Mulabbi ◽  
Chrisostom Ayebazibwe ◽  
Samuel Majalija ◽  
Carrie A. Batten ◽  
Christopher A.L. Oura
Keyword(s):  
Real Time ◽  
Whole Blood ◽  
Bluetongue Virus ◽  
Rna Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
High Seroprevalence ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Karamoja Region

The presence of bluetongue virus (BTV) in indigenous goats from the Karamoja region of northern Uganda was investigated. A total of 300 goats were sampled (serum and whole blood) from five districts within the Karamoja region. The samples were analysed for the presence of bluetongue (BT) antibodies using a commercial Enzyme-linked immunosorbent assay (ELISA) and for the presence of BTV viral RNA by real-time Reverse transcription polymerase chain reaction (RT-PCR), because BTV is an RNA virus. Of the 300 goats tested, 269 (90%) were positive for BTV antibodies, indicating high levels of BTV circulation within the region. Out of the 150 whole blood samples tested for the presence of the virus by real-time RT-PCR, 84 (56%) were positive for BTV RNA. This study, which is the first of its kind in Uganda, showed a high seroprevalence of BT antibodies and active circulation of BTV in a high proportion of goats in the Karamoja region.

Bluetongue Virus RNA Detection by Real-Time RT-PCR in Post-Vaccination Samples from Cattle

2013 ◽  
Vol 62 (2) ◽  
pp. 157-162 ◽  
Author(s):  
I. De Leeuw ◽  
M. Garigliany ◽  
G. Bertels ◽  
T. Willems ◽  
D. Desmecht ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr ◽  
Rna Detection ◽  
Post Vaccination ◽  
Virus Rna

A duplex real-time RT-PCR for the detection of bluetongue virus in bovine semen

2010 ◽  
Vol 169 (1) ◽  
pp. 162-168 ◽  
Author(s):  
Tine Vanbinst ◽  
Frank Vandenbussche ◽  
Eric Dernelle ◽  
Kris De Clercq
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr ◽  
Bovine Semen

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR

2008 ◽  
Vol 152 (1-2) ◽  
pp. 13-17 ◽  
Author(s):  
F. Vandenbussche ◽  
T. Vanbinst ◽  
E. Vandemeulebroucke ◽  
N. Goris ◽  
C. Sailleau ◽  
...  
Keyword(s):  
Real Time ◽  
Bluetongue Virus ◽  
Rt Pcr ◽  
Virus Rna
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