scholarly journals Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR

2017 ◽  
Vol 162 (6) ◽  
pp. 1617-1623 ◽  
Author(s):  
Ji Yeong Noh ◽  
Sun-Woo Yoon ◽  
Doo-Jin Kim ◽  
Moo-Seung Lee ◽  
Ji-Hyung Kim ◽  
...  
Keyword(s):  
Middle East ◽  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Middle East Respiratory Syndrome ◽  
Reverse Transcription Pcr

scholarly journals Real-Time Reverse Transcription-PCR Assay Panel for Middle East Respiratory Syndrome Coronavirus

2013 ◽  
Vol 52 (1) ◽  
pp. 67-75 ◽  
Author(s):  
X. Lu ◽  
B. Whitaker ◽  
S. K. K. Sakthivel ◽  
S. Kamili ◽  
L. E. Rose ◽  
...  
Keyword(s):  
Middle East ◽  
Real Time ◽  
Reverse Transcription ◽  
Middle East Respiratory Syndrome ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0

2019 ◽  
Vol 14 (11) ◽  
pp. 941-948 ◽  
Author(s):  
Leonie-Sophie Hecht ◽  
Angeles Jurado-Jimenez ◽  
Markus Hess ◽  
Hussein El Halas ◽  
Gregor Bochenek ◽  
...  
Keyword(s):  
Middle East ◽  
Reverse Transcription ◽  
Diagnostic Procedure ◽  
Middle East Respiratory Syndrome ◽  
Diagnostic Evaluation ◽  
N Gene ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Confirmatory Assay ◽  
Pcr Targeting

Aim: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. Material & methods: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. Results: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. Conclusion: The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.

scholarly journals US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2

2020 ◽  
Vol 26 (8) ◽  
Author(s):  
Xiaoyan Lu ◽  
Lijuan Wang ◽  
Senthilkumar K. Sakthivel ◽  
Brett Whitaker ◽  
Janna Murray ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Reverse Transcription ◽  
Reverse Transcription Pcr

scholarly journals Triplex Real Time RT-PCR for N1, N2 and RP probes from CDC EUA SARS-CoV-2 diagnosis kit

2020 ◽  
Author(s):  
Byron Freire-Paspuel ◽  
Patricio Vega-Mariño ◽  
Alberto Velez ◽  
Marilyn Cruz ◽  
Miguel Angel Garcia-Bereguiain
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Reverse Transcription ◽  
Clinical Performance ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Speed Up

AbstractCDC protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 3 targets for detection (N1, N2 and RP) labelled with FAM so 3 PCR reactions are required per sample. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with CDC singleplex assay. This protocol could speed up detection and save reagents during current SARS-CoV-2 testing supplies shortage.

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