scholarly journals Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR

2017 ◽  
Vol 162 (6) ◽  
pp. 1617-1623 ◽  
Author(s):  
Ji Yeong Noh ◽  
Sun-Woo Yoon ◽  
Doo-Jin Kim ◽  
Moo-Seung Lee ◽  
Ji-Hyung Kim ◽  
...  
2013 ◽  
Vol 52 (1) ◽  
pp. 67-75 ◽  
Author(s):  
X. Lu ◽  
B. Whitaker ◽  
S. K. K. Sakthivel ◽  
S. Kamili ◽  
L. E. Rose ◽  
...  

2019 ◽  
Vol 14 (11) ◽  
pp. 941-948 ◽  
Author(s):  
Leonie-Sophie Hecht ◽  
Angeles Jurado-Jimenez ◽  
Markus Hess ◽  
Hussein El Halas ◽  
Gregor Bochenek ◽  
...  

Aim: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. Material & methods: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. Results: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. Conclusion: The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.


2020 ◽  
Vol 26 (8) ◽  
Author(s):  
Xiaoyan Lu ◽  
Lijuan Wang ◽  
Senthilkumar K. Sakthivel ◽  
Brett Whitaker ◽  
Janna Murray ◽  
...  

2020 ◽  
Author(s):  
Byron Freire-Paspuel ◽  
Patricio Vega-Mariño ◽  
Alberto Velez ◽  
Marilyn Cruz ◽  
Miguel Angel Garcia-Bereguiain

AbstractCDC protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 3 targets for detection (N1, N2 and RP) labelled with FAM so 3 PCR reactions are required per sample. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with CDC singleplex assay. This protocol could speed up detection and save reagents during current SARS-CoV-2 testing supplies shortage.


Sign in / Sign up

Export Citation Format

Share Document