The complete genome sequence of the lumpy skin disease virus vaccine Herbivac LS reveals a mutation in the superoxide dismutase gene homolog

2019 ◽  
Vol 164 (12) ◽  
pp. 3107-3109 ◽  
Author(s):  
Nicola Douglass ◽  
Anelda Van Der Walt ◽  
Ruzaiq Omar ◽  
Henry Munyanduki ◽  
Anna-Lise Williamson
Keyword(s):  
Superoxide Dismutase ◽  
Disease Virus ◽  
Genome Sequence ◽  
Virus Vaccine ◽  
Complete Genome Sequence ◽  
Skin Disease ◽  
Complete Genome ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Superoxide Dismutase Gene

scholarly journals Complete Genome Sequence of the Lumpy Skin Disease Virus Recovered from the First Outbreak in the Northern Caucasus Region of Russia in 2015

2019 ◽  
Vol 8 (8) ◽  
Author(s):  
Alexander Sprygin ◽  
Yuriy Babin ◽  
Yana Pestova ◽  
Svetlana Kononova ◽  
Olga Byadovskaya ◽  
...  
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Skin Disease ◽  
Complete Genome ◽  
Northern Caucasus ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Monophyletic Origin ◽  
Caucasus Region

We report here the complete genome sequence of a lumpy skin disease virus (LSDV) isolate obtained in the Northern Caucasus region of Russia in 2015. The LSDV/Russia/Dagestan/2015 genome sequence grouped with field LSDV isolates found in Serbia and Greece, suggesting the monophyletic origin of LSDV isolates that recently affected countries in the Northern Hemisphere.

scholarly journals Complete Genome Sequence of a Lumpy Skin Disease Virus Strain Isolated from the Skin of a Vaccinated Animal

2018 ◽  
Vol 6 (22) ◽  
Author(s):  
Ivana Lojkić ◽  
Ivana Šimić ◽  
Nina Krešić ◽  
Tomislav Bedeković
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Skin Disease ◽  
Complete Genome ◽  
Adverse Reactions ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

ABSTRACT Vaccination of cattle against lumpy skin disease (LSD) can cause adverse reactions. Here, we report the first complete genome sequence of an LSD virus strain isolated from the skin of a vaccinated animal. We confirmed that the sequence of the virus used for vaccination remains stable.

scholarly journals Complete Genome Sequence of Lumpy Skin Disease Virus Isolate SERBIA/Bujanovac/2016, Detected during an Outbreak in the Balkan Area

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Ivan Toplak ◽  
Tamaš Petrović ◽  
Dejan Vidanović ◽  
Sava Lazić ◽  
Milanko Šekler ◽  
...  
Keyword(s):  
South Africa ◽  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Skin Disease ◽  
Complete Genome ◽  
Virus Isolate ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Balkan Area

ABSTRACT The lumpy skin disease virus (LSDV) isolate SERBIA/Bujanovac/2016 consists of 150,661 nucleotides and has a 99.95% nucleotide identity with the Neethling Warmbaths LW strain isolated in South Africa in 1999. This is the first complete LSDV genome determined in Serbia and also in the Balkan area.

scholarly journals Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

2017 ◽  
Vol 5 (29) ◽  
Author(s):  
Eirini I. Agianniotaki ◽  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Konstantia E. Tasioudi ◽  
Andy Haegeman ◽  
...  
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Skin Disease ◽  
Complete Genome ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Important Disease

ABSTRACT Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete genome sequence of the first LSDV isolate identified in mainland Europe. LSDV isolate Evros/GR/15 was isolated from the first cases reported on 18 August 2015 in the Evros region, Greece.

scholarly journals Characterization of sheep pox virus vaccine for cattle against lumpy skin disease virus

2014 ◽  
Vol 109 ◽  
pp. 1-6 ◽  
Author(s):  
Eeva S.M. Tuppurainen ◽  
Caroline R. Pearson ◽  
Katarzyna Bachanek-Bankowska ◽  
Nick J. Knowles ◽  
Shadi Amareen ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Vaccine ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

scholarly journals Influence of the lumpy skin disease virus (LSDV) superoxide dismutase homologue on host transcriptional activity, apoptosis and histopathology

2020 ◽  
Vol 101 (6) ◽  
pp. 645-650 ◽  
Author(s):  
Henry Munyanduki ◽  
Nicola Douglass ◽  
Kristy Offerman ◽  
Olivia Carulei ◽  
Anna-Lise Williamson
Keyword(s):  
Superoxide Dismutase ◽  
Disease Virus ◽  
Skin Disease ◽  
Histological Analysis ◽  
Immune Cell ◽  
Cattle Industry ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Knock Out ◽  
Induced Apoptosis

Lumpy skin disease virus (LSDV), a Capripoxvirus, is of economic importance in the cattle industry and is controlled by vaccination. A comparison was made of the host response to the two LSDV vaccines Neethling and Herbivac LS, with reference to the well-studied Orthopoxvirus, modified vaccinia Ankara (MVA), in a mouse model. Because the vaccines differ at the superoxide dismutase homologue (SOD) gene locus, recombinant SOD knock-out and knock-in nLSDV vaccines were constructed and all four vaccines were tested for the induction and inhibition of apoptosis. The SOD homologue was associated both with induction of apoptosis as well as inhibition of camptothecin-induced apoptosis. Histological analysis of chorioallantoic membranes of fertilized hens’ eggs infected with the four different vaccines indicated marked mesodermal proliferation associated with vaccines containing the full-length SOD homologue as well as increased immune cell infiltration. Our findings suggest that the SOD homologue may influence vaccine immunogenicity.

scholarly journals Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 664
Author(s):  
Nicola Douglass ◽  
Henry Munyanduki ◽  
Ruzaiq Omar ◽  
Sophette Gers ◽  
Paidamwoyo Mutowembwa ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Disease Virus ◽  
Skin Disease ◽  
Virus Growth ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Knock Out ◽  
Mdbk Cells

Lumpy skin disease is an important economic disease of cattle that is controlled by vaccination. This paper presents an investigation into the role of the lumpy skin disease virus (LSDV) superoxide dismutase (SOD) homologue on growth and histopathology of the virus both in vitro and in vivo. SOD homologue knock-out and knock-in recombinants (nLSDV∆SOD-UCT and nLSDVSODis-UCT, respectively) were constructed and compared to the Neethling vaccine (nLSDV) for growth in a permissive bovine cell line as well as on fertilized chick chorioallantoic membranes (CAMs). The infected CAMs were scored for histological changes. Deletion of the SOD homologue from LSDV reduced virus growth both in Madin-Darby bovine kidney (MDBK) cells as well as on CAMs. Furthermore, the knockout virus showed reduced inflammation in CAMs and more ballooning degeneration. A pilot experiment was performed in cattle to compare the lesions produced by the different LSDV constructs in the same animal. One animal developed a larger lesion to nLSDV∆SOD-UCT compared to both nLSDVSODis-UCT and nLSDV. Histological analysis of biopsies of these lesions shows less inflammation and necrosis associated with nLSDVSODis-UCT compared to nLSDV and nLSDV∆SOD-UCT. None of the vaccinated animals showed disseminated LSDV disease, indicating that the candidate vaccines are safe for further testing. Our results suggest that the SOD homologue may improve immunogenicity and reduce virulence.

scholarly journals Complete Genome Sequences of the Neethling-Like Lumpy Skin Disease Virus Strains Obtained Directly from Three Commercial Live Attenuated Vaccines

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Andy Haegeman ◽  
Alasdair King ◽  
Bethuel Nthangeni ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Complete Genome ◽  
Animal Health ◽  
Genome Sequences ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Live Attenuated Vaccines ◽  
Virus Strains ◽  
Important Disease

Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete genome sequences of three LSDV strains obtained directly from the live attenuated vaccines: Lumpyvax (MSD Animal Health), Herbivac LS (Deltamune) and Lumpy Skin Disease Vaccine (Onderstepoort Biological Products).

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