Comparisons of coat protein gene sequences show that East African isolates of Sweet potato feathery mottle virus form a genetically distinct group

2000 ◽  
Vol 145 (3) ◽  
pp. 567-574 ◽  
Author(s):  
J. F. Kreuze ◽  
R. F. Karyeija ◽  
R. W. Gibson ◽  
J. P. T. Valkonen
Keyword(s):  
Coat Protein ◽  
Sweet Potato ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Distinct Group ◽  
Mottle Virus ◽  
Gene Sequences ◽  
East African

Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus

2006 ◽  
Vol 63 (1) ◽  
pp. 35-47 ◽  
Author(s):  
A. K. M. N. Haque ◽  
Yoshikazu Tanaka ◽  
Shoji Sonoda ◽  
Masamichi Nishiguchi
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Sweet Potato ◽  
Coat Protein Gene ◽  
Rna Silencing ◽  
Protein Gene ◽  
Mottle Virus

scholarly journals Expression of Recombinant Protein of Coat Protein Gene of Sweet Potato Feathery Mottle Virus in Bacterial Expression System and Production of Polyclonal Antiserum for Its Diagnosis

2021 ◽  
Author(s):  
Makeshkumar Thangaraj ◽  
Jayakrishnan J T
Keyword(s):  
Coat Protein ◽  
Sweet Potato ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Expression System ◽  
Polyclonal Antiserum ◽  
Diagnostic Methods ◽  
Mottle Virus ◽  
Sds Page ◽  
Field Samples

Abstract Sweet potato (Ipomoea batatus L. (Lam), Family Convolvulaceae) is one of the most important tuber crops providing nutritional security because of its high consumption value and medicinal properties, and numerous agro-industrial uses. Sweet potato feathery mottle disease caused by Sweet potato feathery mottle virus (SPFMV) is one of the serious constrains in sweet potato cultivation in India. Effective diagnostic methods need to be developed to solve the problem due to these viruses. As part of the study, infected leaf samples from fields were collected, positive samples were screened for SPFMV using DAC-ELISA and confirmed through PCR. Coat protein gene of SPFMV was PCR amplified, cloned into TA cloning vector and then transformed into Escherichia coli DH5α cells. Positive clones were sub cloned into expression vector pET28A(+) and transformed into DH5α cells. Plasmid DNA from positive clones were isolated and transformed into BL21DE3 cells (NiCo21-DE3 cells). Positive clones were identified and confirmed in-frame position through sequence analysis. Selected colony was grown in Luria both medium at 37oC. Cells were collected and solubility of SPFMV coat protein (CP) was checked through SDS PAGE. Various standardisations were carried out for optimising expression of SPFMV CP and it was observed that 4 hr induction of 1.5 mM IPTG at 25oC gives maximum yield. Using these conditions, cells were grown on large scale and purified the protein (SPFMV CP) using Ni-NTA resin affinity chromatography. Purified protein was checked using SDS PAGE, confirmed the expression using Western Blotting and given for immunization into two New Zealand white rabbits for polyclonal antibody production. Serological tests like ELISA and DIBA were done for confirming the sensitivity and specificity of the raised antibody using field samples of SPFMV infected sweet potato along with healthy plants. Tested samples gave strong positive reactions at dilutions of 1:500 up to 1:6000. Also antibody reacted specifically at a dilution of 1:6000 in ELISA and DIBA. This is the first report of development of polyclonal antiserum against CP of SPFMV through recombinant technology in India and can be useful for the detection of virus from the field-grown samples.

Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus

2001 ◽  
Vol 103 (5) ◽  
pp. 743-751 ◽  
Author(s):  
Y. Okada ◽  
A. Saito ◽  
M. Nishiguchi ◽  
T. Kimura ◽  
M. Mori ◽  
...  
Keyword(s):  
Coat Protein ◽  
Sweet Potato ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Ipomoea Batatas ◽  
Protein Gene ◽  
Mottle Virus

Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)

1996 ◽  
Vol 141 (12) ◽  
pp. 2277-2287 ◽  
Author(s):  
K. Kobayashi ◽  
P. Rabinowicz ◽  
F. Bravo-Almonacid ◽  
M. Helguera ◽  
V. Conci ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Yellow Dwarf ◽  
Gene Sequences

Peanut Stripe Potyvirus Resistance in Peanut (Arachis Hypogaea L.) Plants Carrying Viral Coat Protein Gene Sequences

2004 ◽  
Vol 13 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Colleen M. Higgins ◽  
Rhonda M. Hall ◽  
Neena Mitter ◽  
Alan Cruickshank ◽  
Ralf G. Dietzgen
Keyword(s):  
Coat Protein ◽  
Arachis Hypogaea ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Gene Sequences ◽  
Viral Coat Protein ◽  
Arachis Hypogaea L ◽  
Viral Coat
Sign in / Sign up

Export Citation Format

Share Document