Satellite DNA probes of Alstroemeria longistaminea (Alstroemeriaceae) paint the heterochromatin and the B chromosome, reveal a G-like banding pattern, and point to a strong structural karyotype conservation

PROTOPLASMA ◽  
2021 ◽  
Author(s):  
Tiago Ribeiro ◽  
Magdalena Vaio ◽  
Leonardo P. Félix ◽  
Marcelo Guerra
Keyword(s):  
Banding Pattern ◽  
Satellite Dna ◽  
B Chromosome ◽  
Dna Probes

Efficient identification of marker chromosomes in 27 patients by stepwise hybridization with alpha-satellite DNA probes

1993 ◽  
Vol 91 (2) ◽  
Author(s):  
Rina Plattner ◽  
NylaA. Heerema ◽  
YuriB. Yurov ◽  
CatherineG. Palmer
Keyword(s):  
Satellite Dna ◽  
Dna Probes ◽  
Alpha Satellite ◽  
Marker Chromosomes ◽  
Alpha Satellite Dna

Identification of the origin of centromeres in whole-arm translocations using fluorescent in situ hybridization with α-satellite DNA probes

1991 ◽  
Vol 40 (1) ◽  
pp. 117-120 ◽  
Author(s):  
Avirachan T. Tharapel ◽  
Mazin B. Qumsiyeh ◽  
Paula R. Martens ◽  
Sugandhi A. Tharapel ◽  
James D. Dalton ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Satellite Dna ◽  
Fluorescent In Situ Hybridization ◽  
Dna Probes

C-Banding Pattern and Satellite DNA Localization on the Chromosomes ofOryzomys Flavescens(Rodentia, Cricetidae)

Caryologia ◽  
1988 ◽  
Vol 41 (3-4) ◽  
pp. 323-328 ◽  
Author(s):  
L. Vidal Rioja ◽  
T.G. de Fronza ◽  
R. Wainberg ◽  
N. Brum-Zorrilla ◽  
F. Wallace ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Satellite Dna ◽  
C Banding

Essay on the nucleoli survey by the α- and β-satellite DNA probes of the acrocentric chromosomes in mitogen-stimulated human lymphocytes

2000 ◽  
Vol 43 (2) ◽  
pp. 61-68 ◽  
Author(s):  
Halil Demirtas ◽  
Zühal Candemir ◽  
Nurhan Cücer ◽  
Nalan Imamoglu ◽  
Hamiyet Dönmez ◽  
...  
Keyword(s):  
Satellite Dna ◽  
Human Lymphocytes ◽  
Dna Probes ◽  
Acrocentric Chromosomes

Detection of monosomy in interphase nuclei and identification of marker chromosomes using biotinylated alpha-satellite DNA probes

1991 ◽  
Vol 51 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Marion Kiechle-Schwarz ◽  
Hans-Joachim H. Deckr ◽  
Carol S. Berger ◽  
Heiner H. Fiebig ◽  
Avery A. Sandberg
Keyword(s):  
Satellite Dna ◽  
Dna Probes ◽  
Alpha Satellite ◽  
Interphase Nuclei ◽  
Marker Chromosomes ◽  
Alpha Satellite Dna

Standard Giemsa C-banded karyotype of Russian wildrye (Psathyrostachys juncea) and its use in identification of a deletion–translocation heterozygote

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1262-1270 ◽  
Author(s):  
Jun-Zhi Wei ◽  
William F. Campbell ◽  
Richard R.-C. Wang
Keyword(s):  
Banding Pattern ◽  
Geographic Area ◽  
B Chromosome ◽  
Distal Segment ◽  
Banding Technique ◽  
Translocation Heterozygote ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
Geographical Regions ◽  
Russian Wildrye

Ten accessions of Russian wildrye, Psathyrostachys juncea (Fisch.) Nevski (2n = 2x = 14; NsNs), collected from different geographical regions were analyzed using the C-banding technique. C-banding pattern polymorphisms were observed at all levels, i.e., within homologous chromosome pairs of the same plant, among different individuals within accessions, between different accessions of the same geographic area, and among accessions of different origins. The seven homologous groups varied in the level of C-banding pattern polymorphism; chromosomes A, B, E, and F were more variable than chromosomes C, D, and G. The polymorphisms did not hamper chromosome identification in Ps. juncea, because each chromosome pair of the Ns genome had a different basic C-banding pattern and karyotypic character. A standard C-banded karyotype of Ps. juncea is proposed based on the overall karyotypes and C-bands in the 10 accessions. The C-bands on the Ns-genome chromosomes were designated according to the rules of nomenclature used in wheat. A deletion–translocation heterozygote of Russian wildrye was identified based on the karyotype and C-banding patterns established. The chromosome F pair consisted of a chromosome having the distal segment in the long arm deleted and a translocated chromosome having the distal segment of long arm replaced by the distal segment of the long arm of chromosome E. The chromosome E pair had a normal chromosome E and a translocated chromosome having the short arm and the proximal segment of the long arm of chromosome E and the distal segment of the long arm of chromosome F.Key words: Psathyrostachys juncea, karyotype, Giemsa C-banding, polymorphism, B chromosome.

A molecular analysis of the origin of the Crepis capillaris B chromosome

1994 ◽  
Vol 107 (3) ◽  
pp. 703-708 ◽  
Author(s):  
M. Jamilena ◽  
C. Ruiz Rejon ◽  
M. Ruiz Rejon
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Repetitive Sequences ◽  
B Chromosome ◽  
Dna Probes ◽  
Telomeric Repeats ◽  
Crepis Capillaris ◽  
Telomeric Sequences ◽  
Hybridization Data

The origin of the B chromosome of Crepis capillaris has been studied by using in situ hybridization with different DNA probes. Genomic in situ hybridization (GISH) with DNA from plants with and without Bs as probes indicates that the B chromosome has many DNA sequences in common with A chromosomes, showing no region rich in B-specific sequences. Six additional DNA probes were used to test the possible origin of this B from the standard NOR chromosome (chromosome 3). In the short arm of the NOR chromosome, we detected not only 18 S + 25 S rDNA, but also 5 S rDNA and a specific repetitive sequence from the NOR chromosome (pCcH32); in the heterochromatic bands of the long arm, we found two different repetitive sequences (pCcE9 and pCcD29). In the B chromosome, however, only the 18 S + 25 S rDNA and the telomeric sequences from Arabidopsis thaliana were observed. Our in situ hybridization data with telomeric repeats indicate that the two telomeres of the B are larger than those of the A chromosomes, confirming the isochromosomal nature of this B. Hybridizations of 18 S + 25 S rDNA and telomeric repeats to blots of DNA from plants with and without Bs reveal a high homology between A and B 18 S + 25 S rDNA genes, but some sequence dissimilarities between A and B telomeres. Taken as a whole, these data indicate that the entire B of C. capillaris, although possibly having originated from the standard genome, did not derive directly from the NOR chromosome.

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