Molecular cloning of a cDNA encoding mouse D-aspartate oxidase and functional characterization of its recombinant proteins by site-directed mutagenesis

Amino Acids ◽  
2006 ◽  
Vol 32 (1) ◽  
pp. 69-78 ◽  
Author(s):  
M. Katane ◽  
T. Furuchi ◽  
M. Sekine ◽  
H. Homma
Keyword(s):  
Molecular Cloning ◽  
Recombinant Proteins ◽  
Functional Characterization ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments

2001 ◽  
Vol 14 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Florence Bettsworth ◽  
C�line Monnet ◽  
B�n�dicte Watelet ◽  
Nicole Battail-Poirot ◽  
Bernard Gilquin ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Functional Characterization of the N-glycosylation Sites of Human Acid Sphingomyelinase by Site-Directed Mutagenesis

1997 ◽  
Vol 243 (1-2) ◽  
pp. 511-517 ◽  
Author(s):  
Klaus Ferlinz ◽  
Robert Hurwitz ◽  
Heidi Moczall ◽  
Stephanie Lansmann ◽  
Edward H. Schuchman ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Acid Sphingomyelinase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Glycosylation Sites ◽  
Human Acid

scholarly journals Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens

2021 ◽  
Vol 9 (5) ◽  
pp. 1107
Author(s):  
Wonho Choi ◽  
Yoshihiro Yamaguchi ◽  
Ji-Young Park ◽  
Sang-Hyun Park ◽  
Hyeok-Won Lee ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Physiological Function ◽  
Functional Characterization ◽  
Site Directed Mutagenesis ◽  
In Vitro Assays ◽  
Cell Growth Inhibition ◽  
The Right

Agrobacterium tumefaciens is a pathogen of various plants which transfers its own DNA (T-DNA) to the host plants. It is used for producing genetically modified plants with this ability. To control T-DNA transfer to the right place, toxin-antitoxin (TA) systems of A. tumefaciens were used to control the target site of transfer without any unintentional targeting. Here, we describe a toxin-antitoxin system, Atu0939 (mazE-at) and Atu0940 (mazF-at), in the chromosome of Agrobacterium tumefaciens. The toxin in the TA system has 33.3% identity and 45.5% similarity with MazF in Escherichia coli. The expression of MazF-at caused cell growth inhibition, while cells with MazF-at co-expressed with MazE-at grew normally. In vivo and in vitro assays revealed that MazF-at inhibited protein synthesis by decreasing the cellular mRNA stability. Moreover, the catalytic residue of MazF-at was determined to be the 24th glutamic acid using site-directed mutagenesis. From the results, we concluded that MazF-at is a type II toxin-antitoxin system and a ribosome-independent endoribonuclease. Here, we characterized a TA system in A. tumefaciens whose understanding might help to find its physiological function and to develop further applications.

scholarly journals Porins from plants. Molecular cloning and functional characterization of two new members of the porin family.

1994 ◽  
Vol 269 (41) ◽  
pp. 25754-25760
Author(s):  
K Fischer ◽  
A Weber ◽  
S Brink ◽  
B Arbinger ◽  
D Schünemann ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
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