Development of an improved Amplex Red peroxidation activity assay for screening cytochrome P450 variants and identification of a novel mutant of the thermophilic CYP119

2020 ◽  
Vol 25 (7) ◽  
pp. 949-962
Author(s):  
M. Semih Başlar ◽  
Tuğçe Sakallı ◽  
Gülce Güralp ◽  
Ekin Kestevur Doğru ◽  
Emre Haklı ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Activity Assay ◽  
Amplex Red

Immobilization and activity assay of cytochrome P450 on patterned lipid membranes

2007 ◽  
Vol 355 (4) ◽  
pp. 926-931 ◽  
Author(s):  
Yoshihiro Ueda ◽  
Kenichi Morigaki ◽  
Yoshiro Tatsu ◽  
Noboru Yumoto ◽  
Hiromasa Imaishi
Keyword(s):  
Cytochrome P450 ◽  
Lipid Membranes ◽  
Activity Assay

scholarly journals A High‐Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

2019 ◽  
Vol 58 (30) ◽  
pp. 10114-10119 ◽  
Author(s):  
Tristan de Rond ◽  
Jian Gao ◽  
Amin Zargar ◽  
Markus de Raad ◽  
Jack Cunha ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Cytochrome P450 ◽  
High Throughput ◽  
Mass Spectrometric ◽  
Activity Assay ◽  
Enzyme Activity Assay

Human cytochrome P450 expression in bacteria: Whole-cell high-throughput activity assay for CYP1A2, 2A6 and 3A4

2018 ◽  
Vol 158 ◽  
pp. 134-140 ◽  
Author(s):  
Francisco Esteves ◽  
Diana Campelo ◽  
Philippe Urban ◽  
Sophie Bozonnet ◽  
Thomas Lautier ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
High Throughput ◽  
Activity Assay ◽  
Whole Cell ◽  
Human Cytochrome ◽  
P450 Expression

scholarly journals A High‐Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

2019 ◽  
Vol 131 (30) ◽  
pp. 10220-10225 ◽  
Author(s):  
Tristan de Rond ◽  
Jian Gao ◽  
Amin Zargar ◽  
Markus de Raad ◽  
Jack Cunha ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Cytochrome P450 ◽  
High Throughput ◽  
Mass Spectrometric ◽  
Activity Assay ◽  
Enzyme Activity Assay

scholarly journals Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation

IUCrJ ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 684-694
Author(s):  
Bashu Dev Pardhe ◽  
Hackwon Do ◽  
Chang-Sook Jeong ◽  
Ki-Hwa Kim ◽  
Jun Hyuck Lee ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Structural Analysis ◽  
High Resolution ◽  
Oxidation Rate ◽  
Catalytic Efficiency ◽  
Biosynthetic Pathways ◽  
Biotechnological Applications ◽  
Activity Assay ◽  
Biotransformation Of Xenobiotics

The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The catalytic efficiency of CYP105D18 for papaverine N-oxidation was 1.43 s−1 µM −1. The heme oxidation rate (k) was low (<0.3 min−1) in the presence of 200 mM H2O2. This high H2O2 tolerance capacity of CYP105D18 led to higher turnover prior to heme oxidation. Additionally, the high-resolution papaverine complexed structure and substrate-free structure of CYP105D18 were determined. Structural analysis and activity assay results revealed that CYP105D18 had a strong substrate preference for papaverine because of its bendable structure. These findings establish a basis for biotechnological applications of CYP105D18 in the pharmaceutical and medicinal industries.

Molecular evolution of a cytochrome P450 for the synthesis of potential antidepressant (2R,6R)-hydroxynorketamine

2021 ◽  
Author(s):  
Ansgar Bokel ◽  
Michael C. Hutter ◽  
Vlada B. Urlacher
Keyword(s):  
Cytochrome P450 ◽  
Molecular Evolution ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase ◽  
Effective Synthesis

Engineered cytochrome P450 monooxygenase CYP154E1 enables the effective synthesis of the potential antidepressant (2R,6R)-hydroxynorketamine via N-demethylation and regio- and stereoselective hydroxylation of (R)-ketamine.

Comparison of mechanism-based inhibition of human cytochrome P450 2C19 by ticlopidine, clopidogrel, and prasugrel

Xenobiotica ◽  
2009 ◽  
Vol 00 (00) ◽  
pp. 090901052457079-8
Author(s):  
Y. Nishiya ◽  
K. Hagihara ◽  
A. Kurihara ◽  
N. Okudaira ◽  
N.A. Farid ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Human Cytochrome ◽  
Cytochrome P450 2C19

Deactivation of anti-cancer drug letrozole to a carbinol metabolite by polymorphic cytochrome P450 2A6 in human liver microsomes

Xenobiotica ◽  
2009 ◽  
Vol 00 (00) ◽  
pp. 090901052053001-8
Author(s):  
K. Murai ◽  
H. Yamazaki ◽  
K. Nakagawa ◽  
R. Kawai ◽  
T. Kamataki
Keyword(s):  
Cytochrome P450 ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Cancer Drug ◽  
Anti Cancer ◽  
Cytochrome P450 2A6
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