Evaluation of IVD 3.0 Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Mycobacterium tuberculosis and nontuberculous mycobacteria and its use in routine diagnostics

2018 ◽  
Vol 37 (10) ◽  
pp. 2027-2029 ◽  
Author(s):  
Elio Cenci ◽  
Eugenio Luciano ◽  
Marika Bucaioni ◽  
Monica Rubeca ◽  
Angelica Cesarini ◽  
...  
Keyword(s):  
Nontuberculous Mycobacteria ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Routine Diagnostics ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing

2016 ◽  
Vol 54 (12) ◽  
pp. 2982-2989 ◽  
Author(s):  
Liulin Luo ◽  
Weijia Liu ◽  
Bing Li ◽  
Mengling Li ◽  
Dongdong Huang ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Mycobacterium Abscessus ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization–time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole-genome sequencing analysis: 139 Mycobacterium abscessus subsp. abscessus and 36 Mycobacterium abscessus subsp. massiliense . The research-use-only (RUO) Saramis Knowledge Base database v.4.12 was modified accordingly by adding 40 M. abscessus subsp. abscessus and 19 M. abscessus subsp. massiliense reference spectra to construct subspecies SuperSpectra. A blind test, used to validate the remaining 116 isolates, yielded 99.1% ( n = 115) reliability and only 0.9% ( n = 1) error for subspecies identification. Among the two subspecies SuperSpectra, two specific peaks were found for M. abscessus subsp. abscessus and four specific peaks were found for M. abscessus subsp. massiliense . Our study is the first to report differential peaks 3,354.4 m / z and 6,711.1 m / z , which were specific for M. abscessus subsp. massiliense . Our research demonstrates the capacity of the Vitek MS RUO Saramis Knowledge Base database to identify M. abscessus at the subspecies level. Moreover, it validates the potential ease and accuracy with which it can be incorporated into the IVD system for the identification of M. abscessus subspecies.

scholarly journals A Comprehensive Evaluation of the Bruker Biotyper MS and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Yeasts, Part of the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study, 2012 to 2013

2016 ◽  
Vol 54 (5) ◽  
pp. 1376-1380 ◽  
Author(s):  
He Wang ◽  
Yan-Yan Fan ◽  
Timothy Kudinha ◽  
Zhi-Peng Xu ◽  
Meng Xiao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Among the 2,683 yeast isolates representing 41 different species (25CandidaandCandida-related species and 16 non-Candidayeast species) collected in the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program (2012 to 2013), the Bruker Biotyper MS matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system exhibited significantly higher accuracy rates than the Vitek MS system for identification of all yeast isolates (98.8% versus 95.4%,P<0.001 by Pearson's chi-square test) and for allCandidaandCandida-related species isolates (99.4% versus 95.5%,P< 0.001).

scholarly journals Comparison of Two Commercial Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of Nontuberculous Mycobacteria

2019 ◽  
Vol 152 (4) ◽  
pp. 527-536 ◽  
Author(s):  
Barbara A Brown-Elliott ◽  
Thomas R Fritsche ◽  
Brooke J Olson ◽  
Sruthi Vasireddy ◽  
Ravikiran Vasireddy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nontuberculous Mycobacteria ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Taxonomic Level ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Abstract Objectives This multicenter study’s aim was to assess the performance of two commercially available matrix-assisted laser desorption/ionization time of flight mass spectrometry systems in identifying a challenge collection of clinically relevant nontuberculous mycobacteria (NTM). Methods NTM clinical isolates (n = 244) belonging to 23 species/subspecies were identified by gene sequencing and analyzed using Bruker Biotyper with Mycobacterial Library v5.0.0 and bioMérieux VITEK MS with v3.0 database. Results Using the Bruker or bioMérieux systems, 92% and 95% of NTM strains, respectively, were identified at least to the complex/group level; 62% and 57%, respectively, were identified to the highest taxonomic level. Differentiation between members of Mycobacterium abscessus, M fortuitum, M mucogenicum, M avium, and M terrae complexes/groups was problematic for both systems, as was identification of M chelonae for the Bruker system. Conclusions Both systems identified most NTM isolates to the group/complex level, and many to the highest taxonomic level. Performance was comparable.

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