The presumptive laboratory diagnosis of <em>Clostridium</em> <em>difficile</em> infection is achieved by the means of the detection of a common antigen (glutamate dehydrogenase, GDH) in stool, then confirming the positives either by the detection of toxins A and B or by a molecular test for the detection of pathogenicity <em>locus</em>, encoding for the two toxins and for the binary toxin. A fully automated chemiluminescence system for the GDH antigen (LIAISON® C. difficile GDH) and for the detection of toxins A and B (LIAISON® C. difficile Toxin A and B) (DiaSorin, Gerenzano, Italy) allows for the performance of these tests on large numbers of samples in a short time, ensuring the traceability of the data.