Development and Validation of a High-Performance Liquid Chromatography Method for the Determination of Histamine in Fish Samples Using Fluorescence Detection with Pre-column Derivatization

2020 ◽  
Vol 83 (7) ◽  
pp. 893-901 ◽  
Author(s):  
Ayoub Kounnoun ◽  
Mohamed EL Maadoudi ◽  
Francesco Cacciola ◽  
Luigi Mondello ◽  
Hajar Bougtaib ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
High Performance ◽  
Chromatography Method ◽  
Fish Samples ◽  
Liquid Chromatography Method ◽  
Development And Validation

scholarly journals Simultaneous Determination Of Six Parabens In Cosmetics By A New High Performance Liquid Chromatography Method With Fluorescence Detection

2020 ◽  
Vol 15 (1) ◽  
pp. 27-32
Author(s):  
Pelin Kцseoğlu Yılmaz ◽  
Mehmet Akif Tokat
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
High Performance ◽  
Water Mixture ◽  
Chromatography Method ◽  
Column Temperature ◽  
Liquid Chromatography Method

In this study, a new high performance liquid chromatography method with fluorescence detection was developed and validated for the simultaneous determination of methyl paraben, ethyl paraben, propyl paraben, isopropyl paraben, butyl paraben and benzyl paraben in cosmetics. Separations were achieved using a C18 guard column (2.1 Ч 10 mm, 3 µm) and a C18 analytical column (2.1 Ч 150 mm, 3 µm). Isocratic elution was applied with a mobile phase consisting of 45 % aqueous o-phosphoric acid solution (0.08 %) and 55 % methanol/water mixture (90 : 10 v/v). The excitation and the emission wavelengths were 254 and 310 nm, respectively. Column temperature was fixed at 40 єC. The linear range was 0.50-10.00 μg/mL for all of the parabens. Limits of detection and quantification were in the range of 0.29-0.32 μg/mL and 0.88-0.97 μg/ mL, respectively. Precision and accuracy values were calculated by analysis results of standard solutions at 0.50, 2.50 and 10.00 μg/mL. The developed and validated method was applied for simultaneous quantitative determination of six paraben species in cosmetic tonic and micellar water samples successfully.

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