Grouper nursery development in sea floating net cage through the application of Phyllanthus niruri-Allium sativum flour in feed

Untuk mendukung usaha budidaya ikan kerapu kini tengah digalakkan sistem pendederan intensif dalam keramba jaring apung (KJA) di laut. Salah satu cara untuk menjaga kondisi ikan tetap sehat dalam sistem tersebut yaitu dengan pemberian fitofarmaka seperti meniran-bawang putih. Tujuan penelitian ini adalah untuk mengevaluasi penambahan tepung meniran-bawang putih melalui pakan terhadap status kesehatan dan kinerja produksi benih ikan kerapu cantang pada pendederan dalam keramba jaring apung di laut. Benih ikan kerapu cantang (panjang 8.27 ± 0.16 cm dan bobot 10.89 ± 0.83 g) dipelihara dalam KJA berupa waring 1x1x1.5 m3 dan diberi pakan dengan penambahan tepung meniran-bawang putih 20 + 25 g/kg pakan selama 7 hari dan 14 hari pertama pemeliharaan, serta tanpa tepung meniran-bawang putih (kontrol) sebagai perlakuan. Ikan dipelihara selama 42 hari dan disampling setiap 2 minggu untuk diambil darah serta diukur bobot dan panjangnya. Penambahan tepung meniran-bawang putih selama 14 hari pada pakan ikan kerapu dapat meningkatkan status kesehatan dan kinerja produksi. Kata kunci: ikan kerapu cantang, kinerja produksi, meniran-bawang putih, status kesehatan.

A Mini- review : Effect of Phyllantus niruri L. on Growth and Health of Fish

Phyllanthus niruri L. is one of the herbal ingredients that has been widely used to treat various diseases in humans such as intestinal infections, kidney stones, chronic liver disease, diabetes, hepatitis B, asthma, gonorrhea, bronchitis, syphilis and boost the immune system. The ability of P. niruri in overcoming various diseases is based on its phytochemical content and pharmacological properties. Based on these properties, the plant may possibly be used as a drug to treat diseases in fish. So the purpose of writing this article is to review the extent to which P. niruri can be used as an alternative medicine to treat disease and maintain fish health. Secondary metabolites and phyllanthine as specific compounds present in P. niruri have antibacterial effects for types of bacteria that usually attack fish such as Aeromonas hydrophila, Edwardsiella tarda, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. In addition, it has the effect of being an antioxidant, immunostimulant and can be used for treatment and to increase fish growth. So it can be concluded that P. niruri can be used as an alternative material to maintain health and help fish growth.

TLC-based fingerprinting for Phyllanthus niruri from diverse geographical origins in East and Central Java Indonesia

Phyllanthus niruri L. (meniran), the member of Euphorbiaceae, is a medicinal plant that is commonly found in tropical and sub-tropical areas such as Asia, America, and China. Various factors such as environment, geographical location, harvest time, and post-harvest process can affect the quality of crude drugs produced from P. niruri. The objective of this study was to evaluate the quality of meniran herbs obtained from 15 geographical origins in East and Central Java, Indonesia using Thin Layer Chromatography (TLC) profiles analyzed by chemometrics. TLC was carried out using TLC plate Si Gel 60 GF254 as stationary phase; toluene, ethyl acetate, methanol, 85% formic acid (75:25:25:6) as mobile phase; and visualized using NP/PEG Reagent. The results showed TLC-fingerprinting combined with chemometric (PCA and CA) analyses were able to discriminate the origin of P. niruri from different geographical origins. P. niruri from 15 locations of East and Central Java Indonesia were classified into 5 groups based on their chemical similarity. The samples that are grouped in one cluster have the similar quality of chemical compounds, while the samples in different clusters also have different qualities.

Evaluation of the phytochemical content, antimicrobial and antioxidant activity of Cocos nucifera liquid smoke, Garcinia mangostana pericarp, Syzygium aromaticum leaf, and Phyllanthus niruri L. extracts

Background and Aim: Many plants contain bioactive substances with antibacterial and antifungal properties. The aim of this study was to evaluate the antibacterial and antifungal activity of Cocos nucifera shell liquid smoke (CSL), clove leaf extract (CLE), and mangosteen pericarp extract (MPE) alone and in combination against Escherichia coli and Candida utilis. The antioxidant activity, phenol, saponin, and tannin of CSL, CLE, MPE, and Phyllanthus niruri L. extract were also measured. Materials and Methods: The agar well-diffusion method was used to determine the antimicrobial and antifungal activities of CSL, methanolic MPE, and CLE and their combination CSL+MPE+CLE (COMBI) on bacteria E. coli and fungus (C. utilis). Antioxidant activity was measured by the diphenylpicrylhydrazyl method. Total phenol and total tannin were measured by the Folin–Ciocalteu method and total saponin was measured by the vanillin-sulphate method. Results: The results indicated that phenolic and tannin levels were greater in MPE than in CLE, whereas the saponin content was higher in CLE compared with MPE. Undiluted (100%) MPE exhibited lower antibacterial activity (p<0.05) than chloramphenicol against E. coli, however, undiluted CLE and COMBI showed similar activity compared with chloramphenicol against E. coli. COMBI caused significantly (p<0.05) higher inhibition compared with virginiamycin against E. coli. CSL, MPE, and COMBI exhibited significantly lower antifungal activity (p<0.05) than that of ketoconazole against C. utilis. In contrast, CLE showed improved antifungal activity (p<0.05) compared with ketoconazole. Conclusion: Cocos nucifera liquid smoke, Garcinia mangostana pericarp extract, and Syzygium aromaticum leaf extract, either alone or in combination, have the potential to be used as antibacterial and antifungal agents.

Uji Aktivitas Antipiretik Kombinasi Ekstrak Etanol Herba Meniran (Phyllanthus niruri L.) dan Daun Sambung Nyawa (Gynura procumbens L.) Pada Mencit yang Diinduksi Ragi

<p>Demam merupakan kondisi terjadinya kenaikan suhu tubuh di atas suhu normal. Demam terjadi sebagai bagian dari mekanisme pertahanan tubuh melawan infeksi. Penelitian terdahulu, meniran dan sambung nyawa mengandung flavonoid dan secara ilmiah ekstrak tunggal kedua tanaman tersebut memiliki aktivitas antipiretik. Tujuan penelitian ini adalah untuk mengetahui aktivitas antipiretik kombinasi ekstrak etanol herba meniran dan daun sambung nyawa pada mencit yang diinduksi ragi dan untuk membandingkan dengan ekstrak tunggalnya. Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap. Pengujian antipiretik pada penelitian ini mencit diinduksi ragi dengan dosis 20 mL/kgBB secara subkutan dan diukur suhu badan melalui rektal. Kelompok uji dibagi menjadi 8 kelompok yaitu kelompok kontrol positif (Parasetamol 1,3 mg/20 gram BB mencit), kontrol normal, kontrol negatif (CMC-Na), ekstrak daun sambung nyawa (EDS) mengandung 10 mg/kgBB mencit kuersetin (100%), ekstrak herba meniran (EHM) mengandung 10 mg/kgBB mencit kuersetin (100%); EDS 75% : EHM 25%; EDS 50% : EHM 50%; dan EDS 25% : EHM 75% yang diinjeksikan secara intraperitoneal pada mencit. Pengukuran suhu dilakukan dengan interval waktu 30 menit selama 5 jam. Hasil penelitian menunjukkan bahwa nilai AUC semua kelompok perlakuan memiliki perbedaan yang signifikan (p≤0,05) dengan kontrol negatif, yang artinya ekstrak tunggal maupun ekstrak kombinasi herba meniran dan daun sambung nyawa memiliki aktivitas antipiretik. Ekstrak kombinasi herba meniran dan daun sambung nyawa tidak lebih baik dalam menurunkan demam dibandingkan dengan ekstrak tunggalnya. Dosis tunggal ekstrak daun sambung nyawa memiliki aktivitas antipiretik yang paling baik karena memiliki nilai AUC yang paling kecil (10.549,50).</p>

Ethnobotanical study of medicinal plants used for treating urinary tract problems in eastern Indonesia

Indonesia is one of the wealthiest countries in biological resources that have potential as medicinal plants. Medicinal plants can be an alternative in treating diseases such as urinary tract problems by the people of eastern Indonesia. The study aims to evaluate the use of medicinal plants for various urinary tract problems in east Indonesia, including their efficacy and safety based on the literature review. This research was conducted by interview method and field survey. The data were collected from traditional health practitioners in eastern Indonesia. Data were analyzed using Frequency of Citation (FC) and the Use value (UV). The results showed a total of 222 plants species belonging to 78 families were identified for treating urinary tract problems in east Indonesia. The most prevalent of these was the Euphorbiaceae family. The species which had the highest value were Orthosiphon aristatus (FC 12.52%, UV 0.31), Sericocalyx crispus (FC 7.80%; UV 0.19), Phyllanthus niruri (FC 6.35%; UV 0.16) were the vast majority commonly used plant species in the treatment of urinary tract problems. The most common parts used were leaves (44.87%) and herbs (10.66%). The ethnomedicinal flora in east Indonesia is quite diverse for treating urinary tract problems.

The effect of herbal formula consisting of Curcuma xanthorrhiza, Curcuma longa and Phyllanthus niruri on quality of life: Randomized controlled trial

The herbal ingredients consisting of temulawak (Curcuma xanthorrhiza Roxb.), turmeric (Curcuma longa L.), and meniran (Phyllanthus niruri L.) have been used to improve physical fitness. However, the effect of these herbs on the quality of life is not yet known. This study aims to assess the effect of physical fitness herbal formula on quality of life. This study used a randomized controlled trial design. A total of 100 subjects were given herbal formula and 101 subjects were given a placebo. Herbal formula was made by boiling 4 cups (800 ml) of water until it boils, adding simplicia then reducing the heat, waiting for about 15-30 minutes until only two cups (400 ml) of water remained, filtered and put in a special thermos. The herbs and placebo were taken twice a day for 42 days. Before the study and on day 42th, subjects were examined for their quality of life using the Short Form 36 (SF-36) questionnaire. The results showed that there were no significant changes in the mental component summary and the dimensions of physical function, role of emotions, energy, mental health, social function, and general health. However, in the physical component summary and the dimensions of physical role and pain there was a significant increase in the quality of life (p=0,032; p=0.005; p=0.017). In conclusion, the physical fitness herbal formula can improve the physical component summary of quality of life, especially the dimensions of physical roles and pain.