Entomopathogenic fungal endophyte-mediated tritrophic interactions between Spodoptera littoralis and its parasitoid Hyposoter didymator

2021 ◽  
Author(s):  
P. Miranda-Fuentes ◽  
M. Yousef-Yousef ◽  
P. Valverde-García ◽  
Irene M. Rodríguez-Gómez ◽  
I. Garrido-Jurado ◽  
...  
Keyword(s):  
Tritrophic Interactions ◽  
Spodoptera Littoralis ◽  
Fungal Endophyte ◽  
Hyposoter Didymator

scholarly journals Persistent expression of a newly characterized Hyposoter didymator polydnavirus gene in long-term infected lepidopteran cell lines

2001 ◽  
Vol 82 (4) ◽  
pp. 963-969 ◽  
Author(s):  
Anne-Nathalie Volkoff ◽  
Janick Rocher ◽  
Pierre Cérutti ◽  
Marc C. P. Ohresser ◽  
Yves d’Aubenton-Carafa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Spodoptera Littoralis ◽  
Trichoplusia Ni ◽  
Untranslated Region ◽  
Reading Frame ◽  
Short Open Reading Frame ◽  
Hyposoter Didymator ◽  
Insect Cell Lines ◽  
Lepidopteran Cell Lines

An Hyposoter didymator ichnovirus (HdIV) gene was stably maintained and efficiently transcribed in lepidopteran cell lines more than 3 years after HdIV infection. This K-gene had two introns and the fully spliced cDNA, named K19, comprised a short open reading frame and a long 3′-untranslated region with 13 imperfectly repeated sequences (44 to 102 nt). Transcripts related to the K-gene were detected in several long-term infected cell lines (Sf9, Spodoptera littoralis haemocytes, Trichoplusia ni). Conversely, no transcripts related to seven other viral cDNAs were detected, suggesting that the K-related DNA is selectively retained in long-term infected Sf9 cells. The function of the K-gene product and its association with stably transformed insect cell lines remains to be investigated.

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