Improved Preservation of the Tissue Surrounding Percutaneous Devices by Hyaluronic Acid and Dermatan Sulfate in a Human Skin Explant Model

2009 ◽  
Vol 38 (3) ◽  
pp. 1098-1110 ◽  
Author(s):  
Antonio Peramo ◽  
Cynthia L. Marcelo ◽  
Steven A. Goldstein ◽  
David C. Martin
Keyword(s):  
Hyaluronic Acid ◽  
Human Skin ◽  
Dermatan Sulfate

Glycosaminoglycan biosynthesis in the isolated perfused rat lung

1984 ◽  
Vol 57 (6) ◽  
pp. 1648-1654 ◽  
Author(s):  
P. M. Sampson ◽  
R. B. Boyd ◽  
G. G. Pietra ◽  
A. P. Fishman
Keyword(s):  
Hyaluronic Acid ◽  
Heparan Sulfate ◽  
Dermatan Sulfate ◽  
Rat Lung ◽  
Electron Microscopic ◽  
Chondroitin Sulfates ◽  
Isolated Lung ◽  
Microscopic Studies

The suitability of an isolated lung, perfused under carefully monitored conditions, for the study of the biosynthesis of glycosaminoglycans was examined for the rat lung using either [35S]-sulfate or [6-3H]glucosamine. Metabolic and electron-microscopic studies after 3 h of perfusion showed that under the conditions of this study the isolated lung showed no anatomical or metabolic derangements. All glycosaminoglycans normally synthesized in the intact lung were identified. The predominant glycosaminoglycan was heparan sulfate (40% of total). Approximately 14% of the glucosamine incorporated into the glycosaminoglycans was found in hyaluronic acid. Less than 5% of either label was in heparin. The remainder of the synthesized glycosaminoglycans, with the exception of 10% which could not be identified, consisted of the chondroitin sulfates and dermatan sulfate. The relative proportions of the newly synthesized glycosaminoglycans, including the low amounts of heparin, are similar to those found in isolation of endogenous lung glycosaminoglycans. The isolated perfused rat lung appears to be a useful model for the study of glycosaminoglycan biosynthesis by the intact lung.

THE MUCOPOLYSACCHARIDES OF HUMAN SKIN

1949 ◽  
Vol 27e (1) ◽  
pp. 43-57 ◽  
Author(s):  
R. H. Pearce ◽  
E. M. Watson
Keyword(s):  
Hyaluronic Acid ◽  
Sulphuric Acid ◽  
Human Skin ◽  
Sugar Content ◽  
Amino Sugar ◽  
Acid Fraction ◽  
Fresh Tissue ◽  
Mean Values ◽  
Technical Errors ◽  
Reducing Substances

The mean values and standard deviations for the concentrations of the hyaluronic acid and chondroitin sulphuric acid fractions isolated from 11 specimens of human skin were 24.5 ± 5.7 and 26.2 ± 4.7 mgm. per 100 gm. of fresh tissue, respectively.The individual differences in the concentrations of these substances were masked by the technical errors. The small variations in moisture and ash content of the skin did not contribute appreciably to the uncertainty of the concentration values. The purity of the products, determined by their amino sugar content, approximated 60%, which value compared favorably with those obtained by other workers.The content of hexosamine and uronic acid identified both fractions as acid mucopolysaccharides. The release of reducing substances, including N-acetyl hexosamine, from the hyaluronic acid fraction by hyaluronidase paralleled the action of the enzyme upon hyaluronate from umbilical cord. The chondroitin sulphuric acid fraction, which was relatively resistant to the action of hyaluronidase, was assumed to be identical with the chondroitin sulphatelike substance that Meyer and Chaffee isolated from pig skin.

In vivo Bio-Integration of Three Hyaluronic Acid Fillers in Human Skin: A Histological Study

Dermatology ◽  
2013 ◽  
Vol 228 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Christian Tran ◽  
Pierre Carraux ◽  
Patrick Micheels ◽  
Gürkan Kaya ◽  
Denis Salomon
Keyword(s):  
Hyaluronic Acid ◽  
Human Skin ◽  
Histological Study

Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis

2019 ◽  
Vol 42 (17) ◽  
pp. 2867-2874 ◽  
Author(s):  
Kanokporn Chindaphan ◽  
Kanet Wongravee ◽  
Thumnoon Nhujak ◽  
Thasinas Dissayabutra ◽  
Monpichar Srisa‐Art
Keyword(s):  
Capillary Electrophoresis ◽  
Hyaluronic Acid ◽  
Chondroitin Sulfate ◽  
Dermatan Sulfate ◽  
Online Preconcentration

Oocytes Preserve the Ability of Mouse Cumulus Cells in Culture to Synthesize Hyaluronic Acid and Dermatan Sulfate

1993 ◽  
Vol 160 (2) ◽  
pp. 405-412 ◽  
Author(s):  
Evelina Tirone ◽  
Gregorio Siracusa ◽  
Vincent C. Hascall ◽  
Gaetano Frajese ◽  
Antonietta Salustri
Keyword(s):  
Hyaluronic Acid ◽  
Dermatan Sulfate ◽  
Cumulus Cells ◽  
Cells In Culture
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