alzheimer's disease
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2022 ◽  
Vol 72 ◽  
pp. 101298
Author(s):  
Flavio Bertini ◽  
Davide Allevi ◽  
Gianluca Lutero ◽  
Laura Calzà ◽  
Danilo Montesi

Nano Today ◽  
2022 ◽  
Vol 43 ◽  
pp. 101388
Author(s):  
Linlin Xu ◽  
Yuxun Ding ◽  
Feihe Ma ◽  
Yue Chen ◽  
Guidong Chen ◽  
...  

2022 ◽  
Vol 12 (5) ◽  
pp. 888-896
Author(s):  
Wenjuan Fan ◽  
Chen Xudong ◽  
Sun Yizheng ◽  
Shanshan Wu ◽  
Haili Wang ◽  
...  

Alzheimer’s disease (AD) is a progressive neurologic disorder that impacts a diverse population of older adults. As three-dimensional (3D) models are powerful tools for advancing AD studies, the authors have been developed AD cortical organoids to enable the observation of AD pathology at the cellular, tissue, and organ levels. For creating the model, APPSwe/Ind (APP) and PSEN1 (PS1) mutant genes were transfected into mouse induced pluripotent stem cells (iPSCs) following which the iPSC lines that expressed mutant APP and PS1 proteins were obtained. Then, using modified serum-free suspended embryoid body culture, AD cerebral organoids were made successfully at various ages. The AD model can show AD’s biochemical and pathological alterations, such as overexpressions of Aβ40 and Aβ42 and a decrease of GABAergic interneurons. The proposed model has the potential for implementation in many biomedical applications, including AD drug screening, stem cell transplant, and neuronal tissue engineering.


2022 ◽  
Vol 12 (4) ◽  
pp. 867-872
Author(s):  
Qunwei You ◽  
Wenjie Wang ◽  
Taotao Tao ◽  
Tianyu Wang ◽  
Danhong Zhang ◽  
...  

This study intends to explore miR-129’s effect on cell viability of Alzheimer’s disease by regulating the target gene APP. The hippocampal neurons were assigned into model group (MO group); mimetic group (SI group); inhibitor group (IN group) followed by analysis of hippocampal neuronal cell proliferation and activity, APP protein content, miR-129 expression and cell apoptosis by CCK-8 assay, Western blot method, MTT assay, qRT-PCR and flow cytometry. miR-129 expression of hippocampal neurons in IN group was lowest. Compared with IN and MO groups, SI group had significantly increased miR-129 level and reduced number of hippocampal neuron apoptosis (P < 0.05). Compared with IN group, MO group had significantly reduced cell apoptosis (P < 0.05). SI group had highest number of hippocampal neurons proliferation followed by IN group. SI group had highest OD value followed by MO group and IN group. The cell activity of SI group was higher than that of IN group and MO group (both P < 0.05). Compared with SI group, rat neuron activity in MO group was significantly higher than IN group (P < 0.05). The APP protein expression of hippocampal neuron cells in SI group was lowest followed by MO group and IN group (P < 0.05). In conclusion, the low miR-129 expression can inhibit the activity of hippocampal neurons possibly through up-regulation of APP protein content.


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