Effective biotic elicitation of Ruta graveolens L. shoot cultures by lysates from Pectobacterium atrosepticum and Bacillus sp.

The influence of the polyamines putrescine (Put), spermine (Spr) and spermidine (Spd) on growth and furanocoumarin production was investigated by exogenous addition, at different concentrations, to shoot cultures of Ruta graveolens at different phases of growth. Preliminary studies indicated that addition of Put (20 μM) and Spr (80 μM) had a promotive effect on shoot multiplication rate and number of multiple shoots formed. Spd was toxic, even at lower concentrations. The growth-phase of the culture at the time of exogenous addition of polyamines was found to be an important factor. Put was most effective when added at the lag phase, while Spr was most effective when added in the log phase. Time course studies of growth and furanocoumarin content were carried out for each polyamine and phase of addition. It was seen that maximum production of furanocoumarins (256.8 mg/10 g DW) occurred in the second week when Put was added in the lag phase and 260.5 mg/10 g DW in the fourth week when Spr was added in the log phase. Put addition resulted in a 3.10 fold increase in psoralen, 6.12 in xanthotoxin and 1.46 fold in bergapten production. Spr addition resulted in a 1.31 fold increase in psoralen, 4.11 fold in xanthotoxin and 1.49 fold in bergapten production. Results indicate that alteration of growth and furanocoumarin production kinetics is a combined outcome of choice of polyamine and the phase of culture at the time of exogenous addition. Polyamine addition enabled significant enhancement in production of pharmaceutically important bergapten and xanthotoxin in shoot cultures of Ruta graveolens, which could be explored for commercial production.

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Nematotoxic and phytotoxic activity of Satureja montana and Ruta graveolens essential oils on Pinus pinaster shoot cultures and P. pinaster with Bursaphelenchus xylophilus in vitro co-cultures

Industrial Crops and Products ◽

10.1016/j.indcrop.2015.08.045 ◽

2015 ◽

Vol 77 ◽

pp. 59-65 ◽

Cited By ~ 8

Author(s):

Jorge M.S. Faria ◽

Inês Sena ◽

Cristina Moiteiro ◽

Richard N. Bennett ◽

Manuel Mota ◽

...

Keyword(s):

Essential Oils ◽

Pinus Pinaster ◽

Bursaphelenchus Xylophilus ◽

Shoot Cultures ◽

Ruta Graveolens ◽

Phytotoxic Activity ◽

Satureja Montana

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Development and validation of an efficient low cost bioreactor for furanocoumarin production with Ruta graveolens shoot cultures

Liquid Culture Systems for in vitro Plant Propagation ◽

10.1007/1-4020-3200-5_38 ◽

2005 ◽

pp. 509-524 ◽

Cited By ~ 8

Author(s):

E. Gontier ◽

S. Piutti ◽

A. Gravot ◽

S. Milesi ◽

A. Grabner ◽

...

Keyword(s):

Low Cost ◽

Shoot Cultures ◽

Ruta Graveolens ◽

Development And Validation

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In vitro cultures of Ruta graveolens ssp. divaricata as a potential biotechnological source of phenolic compounds

Zeitschrift für Phytotherapie ◽

10.1055/s-0029-1239891 ◽

2009 ◽

Vol 30 (S 01) ◽

Author(s):

H Ekiert ◽

A Piekoszewska ◽

S Zubek

Keyword(s):

Phenolic Compounds ◽

In Vitro Cultures ◽

Ruta Graveolens

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МЕТАБОЛИТЫ ПРОБИОТИЧЕСКИХ БАКТЕРИЙ ОТВЕЧАЮТ ЗА ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ ПРОБИОТИКА "ПРОФОРТ®"

10.33845/0033-3239-2019-68-9-10-45-47 ◽

2019 ◽

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Broiler Chicks ◽

Bacillus Sp

МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY

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