Development and validation of an efficient low cost bioreactor for furanocoumarin production with Ruta graveolens shoot cultures

Author(s):  
E. Gontier ◽  
S. Piutti ◽  
A. Gravot ◽  
S. Milesi ◽  
A. Grabner ◽  
...  
2020 ◽  
Vol 16 (4) ◽  
pp. 456-464
Author(s):  
Danilo F. Rodrigues ◽  
Hérida R.N. Salgado

Background: A simple, eco-friendly and low-cost Infrared (IR) method was developed and validated for the analysis of Cefepime Hydrochloride (CEF) in injectable formulation. Different from some other methods, which employ organic solvents in the analyses, this technique does not use these types of solvents, removing large impacts on the environment and risks to operators. Objective: This study aimed at developing and validating a green analytical method using IR spectroscopy for the determination of CEF in pharmaceutical preparations. Methods: The method was validated according to ICH guidelines and the quantification of CEF was performed in the spectral region absorbed at 1815-1745 cm-1 (stretching of the carbonyl group of β- lactam ring). Results: The validated method showed to be linear (r = 0.9999) in the range of 0.2 to 0.6 mg/pellet of potassium bromide, as well as for the parameters of selectivity, precision, accuracy, robustness and Limits of Detection (LOD) and Quantification (LOQ), being able to quantify the CEF in pharmaceutical preparations. The CEF content obtained by the IR method was 103.86%. Conclusion: Thus, the method developed may be an alternative in the quality control of CEF sample in lyophilized powder for injectable solution, as it presented important characteristics in the determination of the pharmaceutical products, with low analysis time and a decrease in the generation of toxic wastes to the environment.


2019 ◽  
Vol 65 (2) ◽  
pp. 49-54
Author(s):  
Amalia Miklos ◽  
Amelia Tero-Vescan ◽  
Lénárd Farczádi ◽  
Daniela-Lucia Muntean

AbstractObjective: The purpose of this study was to develop a low-cost, yet sensitive and precise UHPLC method for the quantitative determination of ostarine from dietary supplements (DS) for athletes. The analytical performance of the method was verified on a DS legally acquired from a specialized website for athletes. The uniformity of mass and content of the ostarine DS was also verified.Methods: For the quantitative determination of ostarine a UHPLC method was developed and validated. The separation was performed using a reversed-phase C18 column, using a mixture of 75% methanol: 25% formic acid 0.1% in isocratic elution, at a flow rate of 0.5 ml/min. The uniformity of mass and content of DS was performed following the methodology described in the European Pharmacopoeia 7th Edition.Results: The validated method was specific and linear on the concentration range of 1-25 µg/ml and was precise and accurate at all concentration levels, according to the official guidelines for validating analytical methods. An average mass of 510 mg content was obtained for the ostarine capsules, with an RSD of 2.41%. Regarding the uniformity of the content, an average of 4.65 mg ostarine/capsule was obtained with an RSD of 1.05%.Conclusions: The developed UHPLC method was suitable, rapid, sensitive and allowed quantitative determination of active substance content in a DS with ostarine (92.91% ostarine/capsule from 5 mg ostarine/capsule declared by the manufacturer).


2020 ◽  
Vol 133 (8) ◽  
pp. 2431-2450 ◽  
Author(s):  
Yuying Wu ◽  
Ming Li ◽  
Zhonghu He ◽  
Susanne Dreisigacker ◽  
Weie Wen ◽  
...  

Author(s):  
Domenico Soriero ◽  
Giulia Atzori ◽  
Fabio Barra ◽  
Davide Pertile ◽  
Andrea Massobrio ◽  
...  

Several studies have demonstrated that training with a laparoscopic simulator improves laparoscopic technical skills. We describe how to build a homemade, low-cost laparoscopic training simulator (LABOT) and its validation as a training instrument. First, sixty surgeons filled out a survey characterized by 12 closed-answer questions about realism, ergonomics, and usefulness for surgical training (global scores ranged from 1—very insufficient to 5—very good). The results of the questionnaires showed a mean (±SD) rating score of 4.18 ± 0.65 for all users. Then, 15 students (group S) and 15 residents (group R) completed 3 different tasks (T1, T2, T3), which were repeated twice to evaluate the execution time and the number of users’ procedural errors. For T1, the R group had a lower mean execution time and a lower rate of procedural errors than the S group; for T2, the R and S groups had a similar mean execution time, but the R group had a lower rate of errors; and for T3, the R and S groups had a similar mean execution time and rate of errors. On a second attempt, all the participants tended to improve their results in doing these surgical tasks; nevertheless, after subgroup analysis of the T1 results, the S group had a better improvement of both parameters. Our laparoscopic simulator is simple to build, low-cost, easy to use, and seems to be a suitable resource for improving laparoscopic skills. In the future, further studies should evaluate the potential of this laparoscopic box on long-term surgical training with more complex tasks and simulation attempts.


2015 ◽  
Vol 789-790 ◽  
pp. 626-635
Author(s):  
O. Olatunbosun Ajayi ◽  
E. Okafor Onyemaechi ◽  
Onwudiwe Obumneme

In this paper, we describe the development and validation of the kinematic analysis of a low low-cost 4-link robot the first of its kind in Nigeria. This study comprises the following key tasks which describe the methodology for the robot development: design and kinematic analysis of the robot mechanism, construction/fabrication and assembly of the robot, system development (computer interface with the robot) and experimental validation of the robot control. The goal of this robot is to pick an object and drop the object in a specified position. The algorithm to control the robot on the computer was coded in Java and simulated in MATLAB/Simulink platform. The robot was experimentally verified and the results are presented in this paper. The experimental results show that robot development is feasible in Nigeria. The contribution of this study will enhance and promote robot control and development in Nigeria and developing third world countries.


2012 ◽  
Vol 6 (1) ◽  
pp. 012804 ◽  
Author(s):  
Alexandra Homsy ◽  
Peter D. van der Wal ◽  
Werner Doll ◽  
Roland Schaller ◽  
Stefan Korsatko ◽  
...  

Author(s):  
Christopher Golby ◽  
Ludovica Pippa ◽  
Andrea Aliverti ◽  
Theodoros Arvanitis ◽  
Babu Naidu

HardwareX ◽  
2020 ◽  
Vol 8 ◽  
pp. e00119
Author(s):  
Martin Mapley ◽  
Yidi Lu ◽  
Shaun D. Gregory ◽  
Jo P. Pauls ◽  
Geoff Tansley ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document