Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium

2008 ◽  
Vol 30 (11) ◽  
pp. 1931-1936 ◽  
Author(s):  
Yong Kwon Lee ◽  
Seung Yeul Kim ◽  
Ki Heon Kim ◽  
Bok-Hwan Chun ◽  
Kweon-Haeng Lee ◽  
...  
Keyword(s):  
Soybean Protein ◽  
Human Keratinocytes ◽  
Protein Hydrolysates ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Use of plant protein hydrolysates for varicella virus production in Serum-Free Medium

2005 ◽  
Vol 27 (4) ◽  
pp. 243-248 ◽  
Author(s):  
Bok-Hwan Chun ◽  
Yong Kwon Lee ◽  
Won-Gi Bang ◽  
Namhyun Chung
Keyword(s):  
Virus Production ◽  
Protein Hydrolysates ◽  
Plant Protein ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Varicella Virus

Cell density affects spreading and clustering, but not attachment, of human keratinocytes in serum-free medium

1991 ◽  
Vol 99 (2) ◽  
pp. 387-395
Author(s):  
M. Malcovati ◽  
M.L. Tenchini
Keyword(s):  
Cell Density ◽  
Time Course ◽  
Cluster Formation ◽  
Physicochemical Characterization ◽  
Human Keratinocytes ◽  
Conditioned Media ◽  
Serum Free Medium ◽  
Free Medium ◽  
Spreading Factor ◽  
Serum Free

Attachment, spreading and clustering of second-passage human human keratinocytes in serum-free medium have been evaluated within 24 h after plating, as a function of the density of the inoculum and of time, in two different strains. The results show that attachment is unaffected by cell density and differs significantly from strain to strain. Cell density affects the distribution of attached keratinocytes among three morphologically distinct classes: unspread, spread and clustered cells. The percentage of unspread keratinocytes shows a linear decrease at increasing cell density, and that of spread keratinocytes an increase, resulting from statistically significant increases in the percentages of both single and clustered cells. Spreading on uncoated surfaces appears therefore as an inducible phenomenon. The use of media conditioned by keratinocytes, fibroblasts and HeLa cells shows that keratinocytes specifically secrete a diffusible ‘spreading factor’. We term this phenomenon ‘autocrine induced spreading’. Preliminary physicochemical characterization suggests that a protein could be responsible for the spreading activity of conditioned media. The ‘spreading factor’ seems to act directly on the cells, and not through a modification of the plastic surface of the dishes, since most (greater than 70%) of the spreading activity can be recovered in the conditioned media used in pre-coating experiments. The percentages of clusters follow ‘saturation’ kinetics at increasing cell density, while the percentage of clustered cells increases linearly with the density of inoculum. Time-course experiments show that the rate of spreading is cell density- and strain-independent. The percentages of clusters and of total clustered cells are time-independent, suggesting that cluster formation takes place in suspension.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Adaptation and cultivation of permanent fish cell line CCO in serum-free medium and influence of protein hydrolysates on growth performance

2014 ◽  
Vol 68 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Kristina Radošević ◽  
Bogdanka Dukić ◽  
Martina Andlar ◽  
Igor Slivac ◽  
Višnja Gaurina Srček
Keyword(s):  
Growth Performance ◽  
Cell Line ◽  
Protein Hydrolysates ◽  
Fish Cell Line ◽  
Fish Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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